The effects of stereotactic cerebroventricular administration of albumin, mannitol, hypertonic sodium chloride, glycerin and dextran in rats with experimental brain edema

Ateş, Tuncay; Gezercan, Yurdal; Menekşe, Güner; Türköz, Yusuf; Parlakpınar, Hakan; Ökten, Ali İhsan; Akyuva, Yener; Önal, Selami Çağatay

doi:10.5137/1019-5149.jtn.17231-16.1

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…These results suggest that the biomarkers selected have biological significance and it is reasonable for EA to play an antipyretic role by regulating these biomarkers. In the study of the effects of cerebroventricular administration of hyperoncotic/hyperosmotic agents on edematous brain tissue in rats with experimental head trauma, the levels of MDA and IL-1β decreased after glycerin treatment [29]. These results are consistent with our experimental results.…”

Section: Discussionsupporting

confidence: 91%

Serum Metabolomics Based on GC-MS Reveals the Antipyretic Mechanism of Ellagic Acid in a Rat Model

Xie

Zhu

et al. 2022

Metabolites

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Ellagic acid (EA) is a polyphenol dilactone that has been reported to have antipyretic, anti-inflammatory, anti-tumor, and antioxidant activities, but the mechanism of action has not been reported. In this study, serum metabolomics was used to explore the mechanism of EA on rat fever induced by beer yeast, and to screen out marker metabolites to provide a reference for the antipyretic effect of EA. The acute fever model of male Sprague Dawley rats involved subcutaneous injection with 20% aqueous suspension of yeast (15 mL/kg) in their back. At the same time of modeling, EA was given orally by 10 mL/kg intragastric administration for treatment. During the experiment, the temperature and its change values of rats were recorded, and Interleukin-6 (IL-6), Tumor Necrosis Factor-α (TNF-α), Prostaglandin E2 (PGE2), Cyclic Adenosine Monophosphate (cAMP), Superoxide Dismutase (SOD) and Malondialdehyde (MDA)—six physiological and biochemical indexes of rats—were detected after the experiment. In addition, the hypothalamus of each rat was analyzed by Western blot (WB), and the levels of Phospho Nuclear Factor kappa-B (P-NF-κB P65) and IkappaB-alpha (IKB-α) were detected. Then, the serum metabolites of rats in each group were detected and analyzed by gas chromatograph mass spectrometry and the multivariate statistical analysis method. Finally, when screening for differential metabolites, the potential target metabolic pathway of drug intervention was screened for through the enrichment analysis of differential metabolites. Pearson correlation analysis was used to systematically characterize the relationship between biomarkers and pharmacodynamic indicators. EA could reduce the temperature and its change value in yeast induced fever rats after 18 h (p < 0.05). The level of IL-6, TNF-α, PGE2, cAMP, SOD and MDA of the Model group (MG) increased significantly compared to the Normal group (NG) (p < 0.001) after EA treatment, while the levels of the six indexes in the serum and cerebrospinal fluid of yeast-induced rats decreased. The administration of yeast led to a significant increase in Hypothalamus P-NF-κB P65 and IKB-α levels. Treatment with EA led to a significant decrease in P-NF-κB P65 levels. Moreover, combined with VIP > 1 and p < 0.05 as screening criteria, the corresponding retention time and characteristic mass to charge ratio were compared with the NIST library, Match score > 80%, and a total of 15 differential metabolites were screened. EA administration significantly regulated 9 of 15 metabolites in rat serum. The 15 differential metabolites involved linoleic acid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, galactose metabolism, biosynthesis of unsaturated fatty acids and glycerolipid metabolism. Pharmacodynamic correlation analysis was conducted between 15 different metabolites and six detection indexes. There was a significant correlation between 13 metabolites and six detection indexes. D-(−)-lactic acid, glycerin, phosphoric acid, 5-oxo-L-proline were negatively correlated with TNF-α, and p values were statistically significant except for L-tyrosine. In addition, glycerin was negatively correlated with IL-6, PGE2 and MDA, while phosphoric acid was negatively correlated with IL-6. In conclusion, EA may play an antipyretic anti-inflammatory role through the inhibition of the IKB-α/NF-κB signaling pathway and five metabolic pathways, which may contribute to a further understanding of the therapeutic mechanisms of the fever of EA.

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Section: Discussionsupporting

confidence: 91%

Serum Metabolomics Based on GC-MS Reveals the Antipyretic Mechanism of Ellagic Acid in a Rat Model

Xie

Zhu

et al. 2022

Metabolites

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“…4 However, HS40SC injection as a plasma substitute, similar to albumins, did not affect the transmembrane OP by altering the membrane potential and activating ion channels. 43 HS40SC was speculated to keep the colloid particles and membrane potential in the blood plasma stable. Therefore, the changes in the membrane potential and opening of voltage-gated ion channels substantially affect the transmembrane OP and are therefore important factors to assess the sodium ion-injection-induced damage to the hypertensive endothelial cells.…”

Section: Dovepressmentioning

confidence: 99%

Intravenous Injection of Na Ions Aggravates Ang II-Induced Hypertension-Related Vascular Endothelial Injury by Increasing Transmembrane Osmotic Pressure

Song,

Li,

Gan

et al. 2023

IJN

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Extracellular protein nanoparticles (PNs) and ions perform synergistical functions in the control of transmembrane osmotic pressure (OP) under isotonic conditions. Intravenous injection may disrupt the ion balance and alter PN levels in blood plasma, changing transmembrane OP and damaging vascular endothelial cells. Methods: Na ions were injected into AngII-induced HUVECs to simulate cell injury in vitro, and tail vein infusion of Na ions into hypertensive rats was performed to assess vascular damage. Optical measurements using an intermediate filament (IF) tension probe were conducted to detect indicators related to transmembrane OP. Immunofluorescence, Western blotting and small interfering RNA (siRNA) transfection were employed to investigate inflammasomes and the relationship between Abl2 and inflammation. Results: Electrolyte injections with sodium ions (but not glucose and hydroxyethyl starch) induced the production of ASC and NLRP3 inflammasomes in Ang II-induced HUVECs; this in turn resulted in the disorder of calcium signals, and changes in transmembrane OP and cell permeability. Moreover, injection of Na ions into Ang II-induced HUVECs activated the mechanosensitive protein Abl2, involved in inflammation-induced transmembrane OP changes. A drug combination was identified that could induce OP recovery and block hyperpermeability induced by cytoplasmic inflammatory corpuscles in vivo and in vitro. Conclusion: Changes in extracellular PNs and ions following chemical stimuli (Ang II) participate in the regulation of transmembrane OP. Furthermore, injection of Na ions causes vascular endothelial injury in Ang II-induced cells in vitro and hypertension rats in vivo, suggesting it is not safe for hypertensive patients, and we propose a new drug combination as a solution.

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Tavşanlarda Deneysel Subaraknoı̇d Kanama Modelı̇nde IV %20 Mannı̇tolün Serebral Vazospazm Üzerı̇ne Etkı̇lerı̇

SANRI,

YILMAZ,

ARPA

et al. 2023

Dicle Tıp Dergisi

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Amaç: Taradığımız tüm ingilizce literatürde Subaraknoid Kanama (SAK) tedavisinde sıklıkla kullanılan %20 mannitolün serebral vazospazm üzerine etkilerinin incelenmediğini gördük. Bu nedenle bu çalışmada tavşanlarda deneysel SAK modelinde bir osmotik diüretik ajan olan ve kafa içi basınç artışı sendromu (KI􀇚BAS) durumunda sıklıkla kullanılan intravenöz %20 mannitol uygulamasının serebral vazospazm üzerine etkilerini incelemeyi amaçladık. Yöntemler: Çalışmamızda 28 adet Yeni Zelanda beyaz tavşanı kullanıldı. Tavşanlar Grup 1 (kontrol, n=7),Grup 2 (SAK (+), n=7),Grup 3 (SAK(-)%20 Mannitol (+), n=7), Grup 4 (SAK(+), %20 Mannitol(+), n=7) olmak üzere 4 gruba ayrıldı. Deneysel SAK modeli Grup 2 ve 4’teki tavşanların sisterna magnalarına otolog arteryel kan verilerek SAK oluşturuldu. Grup 3 ve 4’teki tavşanlara 1gr/kg/gün % 20 mannitol 4 eşit doza bölünerek 3 gün süre ile intravenöz olarak verildi. Tüm tavşanlar SAK oluşumunu takip eden 72.saatte sakrifiye edilerek beyin beyin sapı global olarak çıkarılıp fiske edildi. Deneklerin baziller arterlerinden elde edilen kesitler Hematoksilen ve Eosin ile boyanarak mikroskop altında incelendi. Bulgular: Elde edilen baziller arter kesitlerinde damar kalınlığı, damar çapı, lümen çapı, lümen kesit alanı ve damar toplam alanları ölçüldü. Grup 1,3,4 arasında damar duvar kalınlığı yönünden anlamlı fark saptanmadı(p=0,01). Tüm gruplar arasında en fazla damar duvar kalınlığı grup 2’de ölçüldü. Grup 1 ve 3 karşılaştırıldığında baziller arter lümen çapları arasında anlamlı fark saptanmadı (p=0,46). Grup 1,2,4 lümen çapları arasında fark saptanmadı. Baziller arter lümen kesit alanları arasında da anlamlı fark saptanmadı (p=0,08). Sonuç: %20 Mannitol tedavisinin Subaraknoid kanama deneysel modeli sonrası gelişen serebral vazospazm üzerine olumlu veya olumsuz herhangi bir etkisinin olmadığı saptandı.

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The effects of stereotactic cerebroventricular administration of albumin, mannitol, hypertonic sodium chloride, glycerin and dextran in rats with experimental brain edema

Abstract: Cerebroventricular administration of hyperoncotic/hyperosmotic agents provides substantial effects on the treatment of brain edema.

Cited by 3 publications

References 29 publications

Serum Metabolomics Based on GC-MS Reveals the Antipyretic Mechanism of Ellagic Acid in a Rat Model

Serum Metabolomics Based on GC-MS Reveals the Antipyretic Mechanism of Ellagic Acid in a Rat Model

Intravenous Injection of Na Ions Aggravates Ang II-Induced Hypertension-Related Vascular Endothelial Injury by Increasing Transmembrane Osmotic Pressure

Tavşanlarda Deneysel Subaraknoı̇d Kanama Modelı̇nde IV %20 Mannı̇tolün Serebral Vazospazm Üzerı̇ne Etkı̇lerı̇

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