The effects of spermidine synthesis inhibitors on in-vitro plant development

Feirer, Russell P.; Wann, Steven R.; Einspahr, D. W.

doi:10.1007/978-94-009-5171-6_10

Cited by 22 publications

(10 citation statements)

References 20 publications

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“…The results of this study suggest that DCHA, an inhibitor of Spd synthesis in other plant systems [4], is not a specific inhibitor of Spd synthase in this experimental callus system. Thus, DCHA reduced Put and alkaloid titers and was less effective than D F M A and D-Arg in inhibiting Spd titer.…”

Section: Discussionmentioning

confidence: 78%

“…PA also appear to be important for the growth of several plant species [2,8,12,23] and may be essential for embryo development in animals as well [5]. Through the use of inhibitors of PA biosynthesis, Feirer et al [3,4] have suggested that arginine decarboxylase (ADC; an alternative route for PA biosynthesis) [6,32] regulates embryogenesis in carrot [3] and that Spd may play an important role in the growth and development of some plants [4]. Recently, we have found that specific PA can control floral vs. vegetative organogenesis in tobacco thin-layer explants cultured in vitro [14].…”

Section: Introductionmentioning

confidence: 99%

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Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

Tiburcio

Kaur‐Sawhney

Galston

1987

Plant Cell Tiss Organ Cult

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We studied the effects of inhibitors of ornithine decarboxylase (ODC), arginine decarboxylase (ADC) and spermidine synthase (Spd synthase) on organogenesis and the titers of polyamines (PA) and alkaloids in tobacco calli. DL-alpha-diffluromethylarginine (DFMA) and D-arginine (D-Arg), both inhibitors of ADC activity, were more effective than DL-alpha-difluromethylorinithine (DFMO), an inhibitor of ODC, in reducing titers of PA and the putrescine (Put)-derived alkaloids (nornicotine and nicotine). Dicyclohexylammonium sulfate (DCHA), an inhibitor of Spd synthase, was also more efficient than DFMO in reducing PA and alkaloid levels. Root organogenesis is inversely related to the titers of Put and alkaloids. Thus, DFMA and D-Arg, which strongly inhibit Put and alkaloid biosynthesis, markedly promote root organogenesis, while control callus with high Put and alkaloid content showed poor root organization. These results suggest that morphological differentiation is not required for activation of secondary metabolic pathways and support the view that ADC has a major role in the generation of Put going to the pyrrolidine ring of tobacco alkaloids.

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Section: Discussionmentioning

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

Tiburcio

Kaur‐Sawhney

Galston

1987

Plant Cell Tiss Organ Cult

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“…High levels of PA are required during embryogenesis in carrot tissue cultures [4,5] and vegetative and floral bud differentiation in tobacco thin cell-layer cultures [11,24,26,27]. Both embryo and organ differentiation were inhibited when DFMA was added to the cultures.…”

Section: Discussionmentioning

confidence: 99%

“…If large increases of Spd have been associated with floral [11,13] and embryo [5] differentiation in tobacco and carrot, respectively, this investigation indicates that Put can be considered as a good marker of root differentiation in tobacco thin cell-layer explants. In this system, the Put accumulation from day 0 to day 10, with a peak at day 7, occurs before the roots can be observed macroscopically (at day 17).…”

Section: Discussionmentioning

confidence: 99%

Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation

Tiburcio

Gendy

Van

1989

Plant Cell Tiss Organ Cult

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We have used the tobacco thin cell layer 'in vitro' system to evaluate changes in polyamine titers as correlated with root differentiation and with variations in external pH during culture. We show that root differentiation in this system depends on both a rise in putrescine titers and a drop of pH, each of these two factors acting independently. With respect to polyamine titers, the most dramatic changes occur in the levels of putrescine liberated from perchloric acid-soluble conjugates. These titers increase from day 0 to day 7 of culture, reaching almost 2000nmolg -1 fresh weight. Inhibition of putrescine biosYnthesis prevents root initiation, while exogenous putrescine supply reverses this effect. We conclude that putrescine is a good marker for root differentiation.

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“…Spd in particular has been implicated in the SE of tissue culture of D. carota [16,17], Vigna aconitifolia [18], Hevea brasiliensis [19], and during the induction phase in Medicago sativa explants [20]. The addition of spd to the culture medium significantly increases the production of somatic embryos in P. ginseng [14], while in A. angustifolia it was found to reduce cellular growth and allow a morphogenetic evolution of aggregates from the proembryogenic masses (PEM) II to the PEM III stage [8].…”

Section: Introductionmentioning

confidence: 99%

Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora

2008

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The concentration of free and bound polyamines was studied during the somatic embryogenesis induction process in Coffea canephora explants. In the present study we show that when the induction of somatic embryogenesis in C. canephora is carried out under light conditions and in the presence of the plant growth regulator, benzylaminopurine, a cytokinin, a faster response to induction is obtained. In the darkness, the response is delayed for more than 20 days, and the number of embryos is smaller. In the absence of benzylaminopurine no embryogenic response was observed. The pronounced changes in the levels of putrescine, spermidine, and spermine, both free and bound, found in C. canephora suggest that a close correlation exists between polyamine biosynthesis and somatic embryogenesis in C. canephora during a period of cellular differentiation associated with the induction of somatic embryogenesis.

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The effects of spermidine synthesis inhibitors on in-vitro plant development

Cited by 22 publications

References 20 publications

Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation

Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora

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