Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation

Tiburcio, Antonio F.; Gendy, C.; Van, K. Tran Thanh

doi:10.1007/bf00037775

Cited by 72 publications

(29 citation statements)

References 31 publications

(33 reference statements)

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“…An increase in endogenous polyamines in relation to IBA-induced root formation has been observed in mung bean hypocotyl and shoot cuttings (Friedman et al 1982(Friedman et al , 1985Jarvis et al 1983), as well as in relation to de novo root formation in tobacco explants (Tiburcio et al 1989;Torrigiani et al 1989;Altamura et al 1991). Furthermore, the inhibition of polyamine biosynthesis in these systems resulted in a remarkable decrease in rooting; similar responses have also been reported for woody species (Wang & Faust 1986).…”

Section: Introductionsupporting

confidence: 51%

Exogenous polyamines improve rooting of hazel microshoots

Rey

Dı́az-Sala

Rodrı́guez

1994

Plant Cell Tiss Organ Cult

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A strong positive effect of polyamines on rooting of microshoots of adult hazel (Corylus aveUana L., cv. Gironell) is described. The effect of polyamines, both in the root induction solution and in the actual rooting medium, was assessed in order to study the effect on the successive rooting phases. Polyamines improved rooting of indole-3-butyric acid-treated microshoots in a synergistic fashion, perhaps by favouring a better induction of roots, with an acceleration of the response (only half the time required for rooting compared to the control). When applied without indole-3-butyric acid, polyamines had only a limited positive effect on rooting, although longer exposure times and/or higher concentrations could increase their effect. Possible rapid uptake and translocation of polyamines in the xylem in our system is discussed. The results offer a new approach to enhance rooting ability of species that are normally difficult to root.Abbreviations: BM -basal medium, IAA -indole-3-acetic acid, IBA -indole-3-butyric acid, NAA -1-naphthaleneacetic acid, Put -putrescine, Spd -spermidine, Spm -spermine

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Section: Introductionsupporting

confidence: 51%

Exogenous polyamines improve rooting of hazel microshoots

Rey

Dı́az-Sala

Rodrı́guez

1994

Plant Cell Tiss Organ Cult

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“…However, other compounds could also be used as rooting markers, and polyamines have been the most studied in recent times (Baraldi et al 1995;Hausman et al 1995;Heloir et al 1996;Gaspar et al 1997). Thus, a positive relationship between polyamine accumulation and the initial stages of adventitious root formation has been reported in many systems (Friedman et al 1982(Friedman et al , 1985Tiburcio et al 1989;Torrigiani et al 1989;Biondi et al 1990;Altamura 1994). Although the involvement of polyamines in root formation is generally accepted, the specific role they play in this process remains to be elucidated and information about the exact rooting phase in which they are involved is very scarce.…”

Section: Introductionmentioning

confidence: 93%

Peroxidase and polyamine activity variation during thein vitrorooting ofBerberis buxifolia

Arena

Pastur²,

Benavides

et al. 2003

New Zealand Journal of Botany

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Berberis buxifolia is a Patagonian shrub with great economic potential for tinctorial, pharmacological, and food industries. Clonal propagation is possible through in vitro culture and is also useful for metabolite production. However, this species is difficult to root, and to improve this, more knowledge of rhizogenesis processes is needed. Polyamines and peroxidases are useful biochemical markers during analysis of rooting phases for correlation with tissue morphological changes. Therefore, endogenous polyamine (putrescine, spermidine, spermine) changes, peroxidase activity evolution, and morphological development were studied to characterise the in vitro rhizogenesis of microshoots of B. buxifolia and, thus, to define the rooting phases. Polyamine and peroxidase changed significantly during the rooting period, and had opposite behaviours which were directly related to the IBA media content. The lower polyamine concentration and the higher peroxidase activity were found in a treatment with a dark period during the first four days and with IBA in the culture medium. Putrescine was the most abundant polyamine found in B. buxifolia tissues, 14-to 18-fold more than spermidine and spermine, respectively. Therefore, these compounds were used to define the rooting phases: an induction phase (0 to 4-7 days) followed by an expression phase (4-7 to 28 days). The observed changes in the biochemical markers could be correlated with microscopic and macroscopic tissue observations in the microshoots, and the time course of rooting percentage. Successive culture media can be developed including polyamines, or other compounds and environmental conditions, which positively modify the studied biochemical markers behaviour.

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“…Subsequent development, however, appears to be dependent on ODC activity, with spermidine appearing to be the most effective polyamine. Conversely, putrescine, formed by ADC, appears to be important in triggering root formation (Tiburcio et al, 1989).…”

Section: Spain (Aft)mentioning

confidence: 99%