Exogenous polyamines improve rooting of hazel microshoots

Rey, Manuel; Dı́az-Sala, Carmen; Rodrı́guez, Roberto

doi:10.1007/bf00046087

Cited by 20 publications

(8 citation statements)

References 22 publications

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“…For this purpose, we looked at changes in polyamine levels in hormone-treated cuttings in the presence and absence of leaves. Polyamines have been implicated in vegetative rooting of stem cuttings in different species [22,23,[26][27][28]. Thus, consistent with previous reports, putrescine levels significantly increased in the bitter melon cuttings that were about to root; i.e., cuttings with one intact leaf and treated with hormone [23,27,28].…”

Section: Discussionsupporting

confidence: 88%

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Malik¹,

Pérez²,

Kunta³

2012

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The study was conducted to investigate the optimal hormone treatment for rooting in bitter melon and the effect of defoliation on rooting and polyamine levels. Commercial preparation (diluted 1:10 and 1:20) gave extensive rooting within five days after treatment. The presence of leaf with the stem cutting was necessary for optimal rooting as defoliation drastically reduced rooting even when the rooting time was extended from 5 to 10 days. The presence of leaf also promoted the levels of putrescine three days after the hormone treatment just at the time of root emergence. Cuttings that did not have leaf did not show an increase in putrescine levels and did not produce roots. Spermidine levels also increased in the cuttings that rooted but there was no change in the levels of spermine in the cuttings that rooted. It appears that putrescine and spermidine play a role in regulating rooting in bitter melon cuttings. The vegetative propagation conditions described show that it is a practically viable technique for small growers to produce large number of plantlets at the farm for selection and cultivation.

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Section: Discussionsupporting

confidence: 88%

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Malik¹,

Pérez²,

Kunta³

2012

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“…Microshoots exposed to the optimized cobalt were more responsive to the rhizogenic cues provided by auxin than those grown on control medium. Although no previous reports are available on the effect of cobalt on the rooting of microshoots in vitro, our hypothesis of polyamine-mediated response of the metal can be a plausible explanation to our observation as polyamine are reported to enhance the rooting ability of the microshoots (Rey et al 1994).…”

Section: Rooting and Hardeningmentioning

confidence: 70%

Cobalt induced augmentation of in vitro morphogenic potential in Erythrina variegata L.: a multipurpose tree legume

Javed

Anis

2014

Plant Cell Tiss Organ Cult

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An efficient in vitro micropropagation system for Erythrina variegata, a multipurpose tree legume was orchestrated as a tool for evaluating the effect of cobalt on morphogenesis and physiological processes of the plant. Various factors effecting in vitro growth and development were optimized. Among all the different concentrations and combinations evaluated, combination of 5.0 lM 6-benzylaminopurine (BA) and 0.5 lM 1-naphthaleneacetic acid (NAA) supplemented to MS medium induced maximum number (12.9) of shoots per explant with greatest (4.8 cm) average shoot length in 93.6 % cultures. Augmentation of cobalt at lower concentration (50 lM) to the optimized media significantly enhanced the growth parameters (shoot number and shoot length) and chlorophyll content of the cultures, while higher concentrations were detrimental. Rooting of the microshoots was most efficiently induced on MS medium supplemented with 2.5 lM indole-3-butyric acid (IBA) where a maximum of 3.3 roots per shoot with an average root length of 3.2 cm were recorded. Exposure of cultures to optimized cobalt concentration enhanced the rhizogenic competence of the microshoots resulting in a greater percentage (83.5 %) of rooting on the same medium as compared to unexposed cultures (74 %). Genetic stability of the clones exposed to optimum cobalt concentration was established by screening of 570 bands produced by 10 ISSR-PCR primers.

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“…PA plays an important role in the promotion of flower primordia, messenger RNA transcription, and specific protein translation and is a vital physiological index of flower bud induction (Rey et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…6 and 10), and promoted the generation of metabolites, such as polyamine, as a second-messenger medium. PA plays an important role in the promotion of flower primordia, messenger RNA transcription, and specific protein translation and is a vital physiological index of flower bud induction (Rey et al 1994). Meanwhile, with the enhancement of the metabolism of arginine, a significant number of substrates for protein synthesis are produced.…”

Section: Identified Proteins For Acid Metabolism and Phytohormone Sigmentioning

confidence: 99%

Comparative Proteomic Analysis Provides Insights into the Regulation of Flower Bud Differentiation inCrocus SativusL.

Zhang

QingCui

et al. 2016

Journal of Food Biochemistry

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Two-dimensional gel electrophoresis was used to gain a further understanding of the molecular mechanisms involved with flower bud differentiation in Crocus sativus L. A total of 101 proteins exhibited significant differential expression, and 72 proteins were reliably identified. During the process of flower bud differentiation in Crocus sativus L., FBA, TPI, NSE, enolase, LOS2, MDH and succinyl-CoA ligase, provided adequate energy and nutrition to complete flower bud differentiation. Methionine-tRNA ligase and arginine may likely interact with phytohormones and signalling molecules. A number of regulatory and antioxidant-related proteins, such as HSP, chaperonin, APX, CAT, GME, Trx and TpxII, transferred defense related signal molecules in response to various stresses. Several proteins associated with cytoskeleton and protein metabolism, also revealed a higher abundance, including proteins that were critical for fiber development. In conclusion, our results provide novel insights into the molecular basis for flower bud differentiation in Crocus sativus L. PRACTICAL APPLICATIONSCrocus sativus L. is known as "plant gold," and has always been used as a flavor and color in foods, as well as a dye. In the production of Crocus sativus L., flowers are the principal products. At present, florescence of Crocus sativus L., which to be handly picked, is too concentrated and the gradual deterioration of flower quality restrict the development of Crocus sativus L. industry. In this study, the biological functionalities and molecular mechanisms of some key proteins that involved for flower bud differentiation were detected and identified by proteomics analysis. This study laid a theoretical basis for the temporal regulation of blooms, selective breeding and the control of gene regulation in the active biosynthetic components of stigmas. An improved understanding of the impact of key proteins during flowering could potentially provide further scope for optimizing Crocus sativus L. industry.

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Exogenous polyamines improve rooting of hazel microshoots

Cited by 20 publications

References 22 publications

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

The effect of leaf presence on the rooting of stem cutting of bitter melon and on changes in polyamine levels

Cobalt induced augmentation of in vitro morphogenic potential in Erythrina variegata L.: a multipurpose tree legume

Comparative Proteomic Analysis Provides Insights into the Regulation of Flower Bud Differentiation inCrocus SativusL.

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