Possible Role of Light and Polyamines in the Onset of Somatic Embryogenesis of Coffea canephora

Abstract

The concentration of free and bound polyamines was studied during the somatic embryogenesis induction process in Coffea canephora explants. In the present study we show that when the induction of somatic embryogenesis in C. canephora is carried out under light conditions and in the presence of the plant growth regulator, benzylaminopurine, a cytokinin, a faster response to induction is obtained. In the darkness, the response is delayed for more than 20 days, and the number of embryos is smaller. In the absence of benzylaminopurine no embryogenic response was observed. The pronounced changes in the levels of putrescine, spermidine, and spermine, both free and bound, found in C. canephora suggest that a close correlation exists between polyamine biosynthesis and somatic embryogenesis in C. canephora during a period of cellular differentiation associated with the induction of somatic embryogenesis.

Effect of polyamines on in vitro somatic embryogenesis in Momordica charantia L.

“…They demonstrated that Put levels increased 27-32 fold in petiole derived tissue cultures growing in embryo induction medium and Put level fell sharply upon transfer of cultures to embryo differentiation medium. Increase in PA content during induction of SE was also reported by Clelia et al 2008 in Coffea caenophora. Furthermore, increase in PA content was detected in cultures of Citrus sinensis growing in embryo inducing medium.…”

Effect of exogenous arginine on sugarcane (Saccharum sp.) somatic embryogenesis, free polyamines and the contents of the soluble proteins and proline

“…Nevertheless, recent studies have shown the involvement of conjugated (Santa-Catarina et al 2007) and bound (De-la-Peña et al 2008) PAs in early stages of somatic embryo development in Ocotea catharinensis and Coffea canephora, respectively. Therefore, future investigations focused on monitoring conjugated and bound PAs content could help to gain a deeper vision of sugarcane embryogenic process during differentiation-maturation stage.…”

Induction of Indirect Somatic Embryogenesis on Embryonic Axis of TRI2025 Tea Clone

“…Successful induction of somatic embryo in light or dark condition was different among plants. Somatic embryogenesis can be achieved in dark condition (Tahardi et al, 2000;Gomes et al, 2006), dark condition followed by light treatment (Sunandar et al, 2017), or fully in light condition (de-la-Pena et al, 2008;Bakhshaie et al, 2010;Lema-Ruminska & Kulus, 2012). In tea micropropagation, light was also important to induce somatic embryogenesis (Akula et al, 2000, Akula & Dodd, 1998 and were in confirmation of the present results of successful induction of somatic embryogenesis when explants were culture on light condition.…”