Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

Tiburcio, Antonio F.; Kaur‐Sawhney, Ravindar; Galston, Arthur W.

doi:10.1007/bf00044246

Cited by 48 publications

(18 citation statements)

References 32 publications

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“…These results may also imply the possibility of involvement of both ODC and ADC in PA synthesis as has been reported by Tiburcio et al (1987). Similarly, Berta et al (1997) found that thinning of cell walls of tobacco thin cell layer explants after DFMA and DFMO administration could be reversed by the supply of PAs.…”

Section: Discussionsupporting

confidence: 52%

Putrescine Influences Growth and Production of Coumarins in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L. cv. Lucknow Local)

Bais

Sudha

Ravishankar

1999

J Plant Growth Regul

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The effect of putrescine (Put) on the growth and production of two coumarins, esculin and esculetin, in hairy roots of chicory (Cichorium intybus L. cv. Lucknow local) was examined. To study the role of Put on growth and production of coumarins, polyamine inhibitors, namely alpha-dl-difluromethylornithine and alpha-dl-difluromethylarginine were used at 1 mM concentration. Put treatment at 1.5 mM produced a 1.9-fold increase in the growth of hairy roots, as well as the production of esculin and esculetin. The treatments with polyamine (PA) inhibitors resulted in much lower growth and production of coumarins compared with both 1.5-mM Put treatment and the control. Both free and conjugated PAs were studied over the whole culture period, and conjugates of all three PAs, namely Put, spermidine, and spermine, were higher than free PAs throughout the culture period. The treatments with PA inhibitors showed lower levels of endogenous PAs compared with Put-treated samples. The treatment with 1.5 mM Put showed maximum accumulation of endogenous conjugated Put (2,098 +/- 157 nmoles gm(-1) fresh weight). The production of esculin and esculetin was strictly correlated with growth in all treatments. Put at 1.5 mM resulted in greater length of primary root (18.3 +/- 1.4 cm) as compared with the control (11 +/- 0.9 cm) and larger numbers of secondary and tertiary roots.

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Section: Discussionsupporting

confidence: 52%

Putrescine Influences Growth and Production of Coumarins in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L. cv. Lucknow Local)

Bais

Sudha

Ravishankar

1999

J Plant Growth Regul

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“…Changes in PA levels have also been described during root formation in Phaseolus mungo [1], tobacco callus [12,25], corn [18] and apple seedlings [32]. Arginine (a precursor of Put) has been found to be involved in auxininduced root formation in Hibiscus cuttings [31], while exogenous Put was found to act synergistically with auxin in promoting root differentiation in Datura innoxia leaf explants [3].…”

Section: Discussionmentioning

confidence: 99%

Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation

Tiburcio

Gendy

Van

1989

Plant Cell Tiss Organ Cult

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We have used the tobacco thin cell layer 'in vitro' system to evaluate changes in polyamine titers as correlated with root differentiation and with variations in external pH during culture. We show that root differentiation in this system depends on both a rise in putrescine titers and a drop of pH, each of these two factors acting independently. With respect to polyamine titers, the most dramatic changes occur in the levels of putrescine liberated from perchloric acid-soluble conjugates. These titers increase from day 0 to day 7 of culture, reaching almost 2000nmolg -1 fresh weight. Inhibition of putrescine biosYnthesis prevents root initiation, while exogenous putrescine supply reverses this effect. We conclude that putrescine is a good marker for root differentiation.

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“…Endogenous polyamine content varied during the rooting process, and different authors have proposed that they have a role in rhizogenesis (Hausman et al 1995a;Heloir et al 1996;Bellamine & Gaspar 1998;Arena et al 2003), but others do not support their participation in rooting (Tiburcio et al 1987;Biondi et al 1990;Jarvis et al 1983). Some workers have reported that adding polyamine can stimulate or even inhibit the rooting phenomenon depending on the polyamine type, concentration, and opportunity for their addition with regard to the different rooting phases (Martin-Tanguy & Carre 1993;Hausman et al 1995a;Sha Valli Khan et al 1999;Arena et al 2003).…”

Section: Discussionmentioning

confidence: 99%

“…Addition of polyamines in the culture media, and the removal of their effects through specific inhibitors, have already been proposed for several species (Tiburcio et al 1987;Hausman etal. 1994Hausman etal.…”

Section: Introductionmentioning

confidence: 99%

Polyamines and inhibitors used in successive culture media forin vitrorooting inBerberis buxifolia

Arena¹,

Pastur

Benavides

et al. 2005

New Zealand Journal of Botany

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Although Berberis buxifolia has been reported to propagate in vitro, rooting is difficult to achieve during subcultures, with reduced quantity and quality of roots, as is the case with many other woody species. Several cofactors of rhizogenesis have been proposed, and the role of polyamines in rooting has recently acquired relevance. Polyamines can stimulate or inhibit microshoot rooting depending on the type and concentration of polyamine and rooting phase, while polyamine inhibitors can improve rooting in some cases. A study of the in vitro rooting of B. buxifolia using polyamines and a mix of polyamine inhibitors in a two-step culture medium is described here, in which a new successive rooting medium was successfully implemented. While polyamine inhibitors sometimes improved rooting, nutrient medium containing a low polyamine concentration (i.e., 1 µM) enhanced rooting compared with the control medium. The best microshoot rooting response resulted from the addition of 1 µM spermidine during the expression phase in the absence of inhibitors in both rooting phases. Overall, incorporating polyamine in the successive media enhances the quality and quantity of roots in B. buxifolia, thus confirming their role during the induction and expression of the radicle primordia differentiation.

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Effect of polyamine biosynthetic inhibitors on alkaloids and organogenesis in tobacco callus cultures

Cited by 48 publications

References 32 publications

Putrescine Influences Growth and Production of Coumarins in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L. cv. Lucknow Local)

Putrescine Influences Growth and Production of Coumarins in Hairy Root Cultures of Witloof Chicory (Cichorium intybus L. cv. Lucknow Local)

Morphogenesis in tobacco subepidermal cells: Putrescine as marker of root differentiation

Polyamines and inhibitors used in successive culture media forin vitrorooting inBerberis buxifolia

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