The effects of periurethral muscle-derived stem cell injection on leak point pressure in a rat model of stress urinary incontinence

Lee, J. Y.; Cannon, Tracy W.; Pruchnic, Ryan; Fraser, Matthew O.; Huard, Johnny; Mb, Chancellor

doi:10.1007/s00192-002-1004-5

Cited by 158 publications

(111 citation statements)

References 26 publications

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“…5,6,19 The rat is mounted on a vertical tilt table to best match the orientation of the human pelvis with respect to gravity. The spinal cord of the rat is transected at the T8-T9 level to eliminate spinobulbospinal pathways, while maintaining urethral closure mechanisms intact.…”

Section: Assessment Of Sui In Animal Modelsmentioning

confidence: 99%

Animal Models of Female Stress Urinary Incontinence

et al. 2008

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Purpose-Urinary incontinence affects 40% of women in the United States and stress urinary incontinence accounts for a large portion of affected patients. As defined by the International Continence Society, stress urinary incontinence is the involuntary leakage of urine upon effort, exertion, sneezing or coughing. Since the ultimate success of long-term management for any condition is based on an understanding of its pathophysiology, and because the pathophysiology of stress urinary incontinence is incompletely defined, animal models have recently been developed to better understand stress urinary incontinence and develop novel treatment alternatives.Materials and Methods-Several animal models for urethral dysfunction have emerged in the last few years, including those based on pathophysiological theories of urethral sphincter dysfunction that were designed to simulate maternal birth trauma. Other models have focused on the creation of a durable model of dysfunction for investigating novel treatments.Results-Since animals cannot express intent, these animal models have focused on measuring decreased urethral resistance. The most widely used methods are the sneeze test, the tilt table technique and the leak point pressure test. Newer techniques include abdominal leak point pressure, urethral pressure measurement and retrograde urethral perfusion pressure. In addition to the advantages and disadvantages of each technique, all methods measure the composite contribution to urethral resistance from smooth and striated muscle, urethral closure and connective tissue, although none measures intent.Conclusions-We critically reviewed the different models of stress urinary incontinence and urethral dysfunction as well as the different methods of measuring urethral resistance. Keywords urethra; urinary incontinence; stress; female; urodynamics; models; animal Urinary incontinence affects 40% of women in the United States. SUI, the involuntary leakage of urine upon effort, exertion, sneezing or coughing, accounts for a large portion of affected patients. More than 165,000 surgical procedures are performed for SUI in the United States. It is estimated that a third of the procedures that are performed to treat SUI are done in patients with recurrent disease. These currently performed procedures for SUI are based on compensatory and nonphysiological mechanisms. Since the ultimate success of the long-term management of any condition is based on an understanding of its pathophysiology, and because †Correspondence:

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Section: Assessment Of Sui In Animal Modelsmentioning

confidence: 99%

Animal Models of Female Stress Urinary Incontinence

et al. 2008

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“…Stem cell therapy appears to be a promising technique to regenerate a deficient urethral sphincter. Lee Hams F-10 20% fetal bovine serum chick embryo extract [61] Hams F-12 15% fetal bovine serum - [90] M199 10% fetal bovine serum - [91] Modified custom-made MCDB medium 20% fetal bovine serum bFGF [36] significantly improved the leak point pressure in treated animals after 4 weeks [69]. Their group also proved improvement of sphincter contractility after allogenic muscle-derived progenitor cells injection into the denervated rat urethra [70].…”

Section: Regeneration Of Urinary Sphinctermentioning

confidence: 95%

Adult stem cells derived from skeletal muscle — biology and potential

2013

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Skeletal muscle contains at least two distinct populations of adult stem cells — satellite cells and multipotent muscle-derived stem cells. Monopotential satellite cells are located under the basal lamina of muscle fibers. They are capable of giving rise only to cells of myogenic lineage, which play an important role in the processes of muscle regeneration. Multipotent muscle-derived stem cells are considered to be predecessors of the satellite cells. Under proper conditions, both in vitro and in vivo, they undergo myogenic, cardiogenic, chondrogenic, osteogenic and adipogenic differentiation. The main purpose of the present article is to summarize current information about adult stem cells derived from skeletal muscle, and to discuss their isolation and in vitro expansion techniques, biological properties, as well as their potential for regenerative medicine.

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“…Knowledge of the pathways of peripheral innervation is an important tool to study EUS function in preclinical studies (Cruz & Downie 2005;Jiang et al 2009aJiang et al , 2009bKamo & Hashimoto 2007;Kamo et al 2009;Kamo et al 2003;Kerns et al 2000;Lee et al 2003;Peng et al 2008aPeng et al , 2008b). Animal models have been used to estimate the damage to nerves during simulated childbirth and to test pharmacological and neuromodulation strategies to promote neuroregeneration and recovery of EUS function Cannon et al 2002;Jiang et al 2009aJiang et al , 2009bPeng et al 2008a;Streng et al 2001;Thor 2003;Thor et al 1989;Thor et al 2007;Vera & Nadelhaft 2001).…”

Section: Innervation Of the Eusmentioning

confidence: 99%