The Effects of Oxidative Stress on Outcomes of Assisted Reproductive Techniques

Oral, Onur; Kutlu, Tayfun; Aksoy, Evrim Emre; Fıçıcıoğlu, Cem; Uslu, Hüsamettin; Tuğrul, Semih

doi:10.1007/s10815-005-9010-4

Cited by 41 publications

(41 citation statements)

References 15 publications

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“…While several studies reported a positive correlation between FF ROS level and pregnancy rate following an IVF treatment [6,7], others found either no correlation [8,9], or demonstrated a negative correlation between ROS levels and reproductive functions [10][11][12]. We believe that this inconsistency derived from the different study methodology, which examine ROS from different FF sources, i.e.…”

Section: Introductionmentioning

confidence: 93%

Reactive oxygen species in follicular fluid may serve as biochemical markers to determine ovarian aging and follicular metabolic age

Elizur

Lebovitz

Orvieto

et al. 2014

Gynecological Endocrinology

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Section: Introductionmentioning

confidence: 93%

Reactive oxygen species in follicular fluid may serve as biochemical markers to determine ovarian aging and follicular metabolic age

Elizur

Lebovitz

Orvieto

et al. 2014

Gynecological Endocrinology

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“…Uncontrolled and excessive production of ROS, however, appears to have a significant role as one of the major factors leading to an infertile status (Agarwal et al, 2003. The role of ROS and antioxidants in relation to female reproductive function has, in contrast, received relatively little attention, although there is some evidence of both physiological and pathological effects (Attaran et al, 2000;Pasqualotto et al, 2004;Das et al, 2006;Oral et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

The role of enzymatic antioxidants detected in the follicular fluid and semen of infertile couples undergoing assisted reproduction

et al. 2009

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The follicular fluid environment surrounding the oocytes may play a critical role in fertilization and subsequent embryo development. The goal of our study was to evaluate the oxidative stress markers in the semen, blood serum, and follicular fluid of couples undergoing Intracytoplasmic Sperm Injection (ICSI). Two hundred and eight infertile couples underwent ICSI and the levels of superoxide dismutase (SOD) and catalase and lipid peroxidation (LPO) were evaluated. Semen Catalase was highly correlated with fertilization and cleavage rates, but not with pregnancy rates. Fertilization and cleavage rates were correlated with the levels of SOD and Catalase in the follicular fluid. After adjusting for age, a negative correlation was detected between LPO levels in follicular fluid and pregnancy rates. Follicular fluid LPO levels may be a marker as a metabolic activity within the follicle need for establishing a pregnancy.

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“…The results of a study by Pasqualotto et al (14) showed parallel results, as pregnant women after IVF treatment had higher lipid peroxidation (LPO) products compared to nonpregnant women. However, Oral et al (12) found no significant relationship between follicular fluid malondialdehyde levels and fertilization rates. Accordingly, Jozwick et al (29) reported no significant correlation between IVF outcome and different oxidative stress marker concentrations.…”

Section: Discussionmentioning

confidence: 97%

“…It is found in high concentrations in the cytoplasm, nucleus and mitochondria (11). Oxidative stress is believed to affect reproductive functions (12). The effect of oxidative stress on the reproductive potential of men has been investigated extensively worldwide.…”

Section: Introductionmentioning

confidence: 99%

Effect of Follicular Fluid NO, MDA and GSH Levels on in vitro Fertilization Outcomes

Yalçınkaya

Çakıroğlu

Döğer

et al. 2013

J Turkish German Gynecol Assoc

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Objective:To investigate the biochemical markers such as nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH), indicators of the oxidative status of the follicle, to predict the outcome of in vitro fertilization. Material and Methods: Follicular aspirates of dominant follicleswere collected during oocyte retrieval. Biochemical analyses of NO, MDA and GSH were performed on all aspirates. Results:When the successful and unsuccessful pregnancy groups were compared in terms of NO, MDA and GSH, follicular fluid MDA was significantly higher (p=0.001) and follicular fluid NO level was significantly lower (p=0.039) in the pregnant group. Correlation analysis between oxidative stress and IVF parameters showed that MDA had a positive weak correlation with the number of grade 1 embryos (r=0.271, p=0.033) and fertilization rate (r=0.263, p=0.039). ROC curve analysis found that malondialdehyde has an area under the curve of 0.74 and can predict pregnancy with high sensitivity. Conclusion:As malondialdehyde was significantly different in pregnant and non-pregnant women and had a good sensitivity profile in predicting pregnancy, it may be considered a marker for predicting IVF success. (J Turkish-German Gynecol Assoc 2013; 14: 136-41) Key words: Follicular fluid, malondialdehyde, nitric oxide, glutathione, in vitro fertilization, pregnancy Received: 22 April, 2013 Accepted: 21 May, 2013 Amaç: Folikül oksidatif durumunun belirteci olan nitrik oksit (NO), malondialdehit (MDA) ve redükte glutatyon (GSH) gibi kimyasal belirteçlerin in vitro fertilizasyon başarısına etkisinin araştırılması. Gereç ve Original Investigation 136 IntroductionInfertility is defined as the inability to conceive for at least one year despite having regular sexual intercourse without using any contraception method. There are different causes of infertility. Nearly 40-50% of infertility problems are estimated to be of female origin and approximately 30% of cases are of male origin. A further 20-25% of couples suffer from unexplained factors (1). The pathophysiology of unexplained infertility is still a scientific question (2). In order to find some answers for unexplained cases, several investigators are trying to develop new, non-invasive biochemical markers that may affect gamete and embryo quality. Oxidative stress is being investigated as a causative marker in this manner. Studies on the pathophysiology of unexplained infertility have indicated that oxidative stress may be involved as an underlying factor (3, 4). The maintenance of homeostasis in cells requires a complex interaction between prooxidants and antioxidants. Oxidative stress occurs as a result of a shift in this balance between prooxidants and antioxidants towards excess free radical formation (5 Abstract Özet is malondialdehyde (MDA). Since it is a stable end product, it can be used as a cumulative measure of lipid peroxidation (6). Nitric oxide (NO) is an inorganic, short-lived free radical gas that is synthesized from L-arginine via NO synthases. It has various ...

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The Effects of Oxidative Stress on Outcomes of Assisted Reproductive Techniques

Abstract: Malondialdehyde can be used as a marker of oxidative stress and a potential marker in predicting assisted reproductive techniques outcome.

Cited by 41 publications

References 15 publications

Reactive oxygen species in follicular fluid may serve as biochemical markers to determine ovarian aging and follicular metabolic age

Reactive oxygen species in follicular fluid may serve as biochemical markers to determine ovarian aging and follicular metabolic age

The role of enzymatic antioxidants detected in the follicular fluid and semen of infertile couples undergoing assisted reproduction

Effect of Follicular Fluid NO, MDA and GSH Levels on in vitro Fertilization Outcomes

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