Effect of Follicular Fluid NO, MDA and GSH Levels on in vitro Fertilization Outcomes

Abstract

Objective:To investigate the biochemical markers such as nitric oxide (NO), malondialdehyde (MDA) and reduced glutathione (GSH), indicators of the oxidative status of the follicle, to predict the outcome of in vitro fertilization. Material and Methods: Follicular aspirates of dominant follicleswere collected during oocyte retrieval. Biochemical analyses of NO, MDA and GSH were performed on all aspirates. Results:When the successful and unsuccessful pregnancy groups were compared in terms of NO, MDA and GSH, follicular fluid MDA was significantly higher (p=0.001) and follicular fluid NO level was significantly lower (p=0.039) in the pregnant group. Correlation analysis between oxidative stress and IVF parameters showed that MDA had a positive weak correlation with the number of grade 1 embryos (r=0.271, p=0.033) and fertilization rate (r=0.263, p=0.039). ROC curve analysis found that malondialdehyde has an area under the curve of 0.74 and can predict pregnancy with high sensitivity. Conclusion:As malondialdehyde was significantly different in pregnant and non-pregnant women and had a good sensitivity profile in predicting pregnancy, it may be considered a marker for predicting IVF success. (J Turkish-German Gynecol Assoc 2013; 14: 136-41) Key words: Follicular fluid, malondialdehyde, nitric oxide, glutathione, in vitro fertilization, pregnancy Received: 22 April, 2013 Accepted: 21 May, 2013 Amaç: Folikül oksidatif durumunun belirteci olan nitrik oksit (NO), malondialdehit (MDA) ve redükte glutatyon (GSH) gibi kimyasal belirteçlerin in vitro fertilizasyon başarısına etkisinin araştırılması. Gereç ve Original Investigation 136 IntroductionInfertility is defined as the inability to conceive for at least one year despite having regular sexual intercourse without using any contraception method. There are different causes of infertility. Nearly 40-50% of infertility problems are estimated to be of female origin and approximately 30% of cases are of male origin. A further 20-25% of couples suffer from unexplained factors (1). The pathophysiology of unexplained infertility is still a scientific question (2). In order to find some answers for unexplained cases, several investigators are trying to develop new, non-invasive biochemical markers that may affect gamete and embryo quality. Oxidative stress is being investigated as a causative marker in this manner. Studies on the pathophysiology of unexplained infertility have indicated that oxidative stress may be involved as an underlying factor (3, 4). The maintenance of homeostasis in cells requires a complex interaction between prooxidants and antioxidants. Oxidative stress occurs as a result of a shift in this balance between prooxidants and antioxidants towards excess free radical formation (5 Abstract Özet is malondialdehyde (MDA). Since it is a stable end product, it can be used as a cumulative measure of lipid peroxidation (6). Nitric oxide (NO) is an inorganic, short-lived free radical gas that is synthesized from L-arginine via NO synthases. It has various ...

Reactive oxygen species in follicular fluid may serve as biochemical markers to determine ovarian aging and follicular metabolic age

“…However, while both Oral et al [8] and Jozwick et al [9] found no significant correlation between IVF outcome and ROS markers, Das et al [10] demonstrated a negative correlation between ROS levels and embryo quality. Moreover, Yalç ınkaya et al [11] found significantly higher intra-follicular NO levels in the non-pregnant group compared to the pregnant group, which did not correlate to other IVF cycle variables, such as, the number of mature oocytes, fertilization rate, or embryo grading. Of notice, none of the aforementioned studies have followed the outcome of a single oocyte and related it to its own FF/microenvironment.…”

“…While several studies reported a positive correlation between FF ROS level and pregnancy rate following an IVF treatment [6,7], others found either no correlation [8,9], or demonstrated a negative correlation between ROS levels and reproductive functions [10][11][12]. We believe that this inconsistency derived from the different study methodology, which examine ROS from different FF sources, i.e.…”

“…We believe that this inconsistency derived from the different study methodology, which examine ROS from different FF sources, i.e. pooled FF from several follicles [6][7][8][9]12], dominant follicles [11], or single follicles with the exclusion of follicles when FF contained more than one oocyte or no oocyte [6,10] or included an oocyte with abnormal morphology [10].…”

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

“…Thus, one of the major objectives of the present study was to determine the influences of melatonin on the oxidative status of pre-ovulatory follicles by measuring the levels of MDA, a reliable marker of intra-cellular stress (Parlakpinar et al 2013), as well as the levels of different antioxidants in the same ovarian follicular extracts of the experimental carp. A high MDA level denoted an elevated oxidative stress (Yalçınkaya et al 2013) in the pre-ovulatory follicles of the control fish. On the other hand, a significant reduction in the level of MDA and the stimulation of enzymatic (SOD, CAT, GPx, GRd and GST) as well as Melatonin and ovaprim actions on carp oocytes non-enzymatic (GSH) antioxidants in the follicular extracts of the melatonin-treated carp collectively demonstrated antioxidative actions of melatonin in the pre-ovulatory follicles of the concerned fish.…”

Osthole, a Natural Coumarin Improves Cognitive Impairments and BBB Dysfunction After Transient Global Brain Ischemia in C57 BL/6J Mice: Involvement of Nrf2 Pathway

“…One of the most important harmful effects of free radical attack is the oxidation of unsaturated fatty acids, known as lipid peroxidation. MDA, one of the typical end products of lipid peroxidation, can be used as a cumulative measure of lipid peroxidation [32]. SOD, one of important endogenous antioxidants, protecting cells exposed to oxygen.…”