The effects of leguminous supplementation on ammoniated rice straw based completed feed on nutrient digestibility on in vitro microbial protein synthesis

Abstract

Legume is the best source of protein to ruminant. Tannin content in legumes can provide protein by pass for ruminant. This study was conducted to determine the effect supplementation of legumes (Leucaena leucocephala) as substitute of concentrate on nutrient digestibility and microbial protein synthesis of the completed feed based on ammoniated rice straw. The study was conducted in an in vitro used a randomized block design where there were 3 treatments dan 5 replications. The treatments were A. 40% ammoniated rice straw + 60% concentrate, B. 40% ammoniated rice straw + 50% concentrate + 10% Leucaena leucocephala, C. 40% ammoniated rice straw + 40% concentrate +10, + 20% Leucaena leucocephala. The results showed that the addition of of Leucaena leucocephala had increased the digestibility of DM, OM, CP and microbial protein synthesis (p< 0.05). Increased doses of Leucaena leucocephala until 20% decreased digestion of nutrition compared with dose 10% but was still higher than control. In conclusion, the supplementation of Leucaena leucocephala on completed feed based on ammoniated rice straw in vitro can increase nutrient degradability. Supplementation of 10% and 20% Leucaena leucochepala were suitable to be used for further studies, therefore in vivo experiment is required to study the effects on animal production.

Effectiveness of herbal plants on rumen fermentation, methane gas emissions, in vitro nutrient digestibility, and population of protozoa

“…If the feed lacks protein or is high in proteins that escape degradation, the rumen NH 3 concentration will be low (lower than 50 mg/L or 3.57 mM), and the growth of rumen organisms will be slow. Conversely, if protein degradation is faster than microbial protein synthesis, NH 3 accumulates and exceeds its optimum concentration [ 39 ]. The main factor affecting the use of NH 3 in rumen fluid is the availability of crude fiber for rumen microorganisms [ 40 ].…”

Impact of Tithonia diversifolia and Pennisetum purpureum-based Ration on Nutrient Intake, Nutrient Digestibility and Milk Yield of Etawa Crossbreed Dairy Goat

“…Tannins in T. diversifolia also protect protein from rumen breakdown by forming a protein-tannin complex link in the small intestine, ensuring that protein does not degrade in the rumen and instead enters the small intestine, where it can be best utilized by the ruminant body for milk production. The amount of protein and amino acids digested directly by enzymes and absorbed in the small intestine can be increased by feeding protein that is not digested in the rumen, which can enhance milk and body protein synthesis (Widyobroto et al 1995;Henson et al 1997;Putri et al 2019;Zain et al 2019).…”

Improving Rumen Fermentation Characteristics and Nutrient Digestibility by Increasing Rumen Degradable Protein in Ruminant Feed using Tithonia diversifolia and Leucaena leucocephala

“…Beside that, higher sulfur content from T. diversifolia and L. leucocephala are essential for microbial protein synthesis. Similarly, Zain et al (2019) reported that due to the presence of significant microbial growth factors; sulfur and calcium minerals in L. leucocephala, especially for cellulolytic microbial growth and fungi; and the availability of nutrients for optimal microbial growth, the inclusion of legumes, specifically L. leucocephala, increases microbial protein synthesis. In addition, legumes supplied enough organic matter for microbial protein synthesis .…”