Rice straw as ruminant feed has low digestibility makes ruminants difficult to digest. Since ammoniated rice straw can increase its digestibility, it is necessary to supplement it with Gliricidia sepium. This research aimed to determine the effect of Gliricidia sepium supplementation on decreasing methane gas production and improving nutrient digestibility in complete ration with ammoniated rice straw. The treatments of this research were: A = 40% ammoniated rice straw + 60% concentrate, B = 40% ammoniated rice straw + 50% concentrate + 10% Gliricidia sepium, C = 40% ammoniated rice straw + 40% concentrate + 20% Gliricidia sepium, D = 40% ammoniated rice straw + 30% concentrate + 30% Gliricidia sepium. This research showed supplementation of G. sepium significantly decreased methane gas production from 27.22 mM to 13.13 mM and the number of protozoa from 6.3 x 105 cell/ml rumen fluid to 4.7 x 105 cell/ml rumen fluid. Gliricidia sepium supplementation increased digestibility significantly (P < 0.05). Digestibility of dry matter increased from 58.83% to 68.54% and digestibility of organic matter also increased from 59.50% to 69.50%. Total VFA (Volatile Fatty Acid) concentration did not differ significantly (P > 0.05) among treatments. Higher levels of G. sepium supplementation increased N-NH3 concentration from 7.33 mM to 10.50 mM and microbial protein synthesis from 74.33 mg/100ml rumen fluid to 108.25 mg/100ml rumen fluid. The treatment had a significant effect (P <0.05) on propionate production and the ratio of acetate: propionate. It can be concluded that 30% of Gliricidia sepium supplementation decreased methane gas production and the number of protozoa. Besides, 30% of Gliricidia sepium supplementation increased digestibility and rumen fermentation.

Legume is a feed ingredient that contains high protein for ruminants. The tannin content in legumes can provide a bypass protein for ruminants. This study was done to find out the effect of legume supplementation (Leucaena leucocephala) on fiber digestibility, characteristic rumen, protozoa population and methane production from total mixed ration (TMR) based on ammoniated rice straw. The study was conducted in vitro using a randomized block design with 3 treatments and 5 replications. The treatment was A. 40% ammoniated rice straw + 60% concentrate, B. 40% ammoniated rice straw + 50% concentrate + 10% L. leucocephala, C. 40% ammoniated rice straw + 40% concentrate +10, + 20% L.leucocephala.The results showed that the addition of L. leucocephala increased digestibility of NDF, ADF, and Cellulose, RUP (rumen undegradable protein) and reduced protozoa population and methane production (p <0.05). Increased doses of L.leucocephala up to 20% reduce fiber digestibility of feed substances compared to a dose of 10% but are still higher than controls. The results of this study concluded that L.leucocephala supplementation in TMR based on ammoniated rice straw in vitro improved digestibility, fermentability, and reduced methane gas production. Supplementation of 10% and 20% L. leucocephala needs further research, to see the effect on livestock in vivo.

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Legume is the best source of protein to ruminant. Tannin content in legumes can provide protein by pass for ruminant. This study was conducted to determine the effect supplementation of legumes (Leucaena leucocephala) as substitute of concentrate on nutrient digestibility and microbial protein synthesis of the completed feed based on ammoniated rice straw. The study was conducted in an in vitro used a randomized block design where there were 3 treatments dan 5 replications. The treatments were A. 40% ammoniated rice straw + 60% concentrate, B. 40% ammoniated rice straw + 50% concentrate + 10% Leucaena leucocephala, C. 40% ammoniated rice straw + 40% concentrate +10, + 20% Leucaena leucocephala. The results showed that the addition of of Leucaena leucocephala had increased the digestibility of DM, OM, CP and microbial protein synthesis (p< 0.05). Increased doses of Leucaena leucocephala until 20% decreased digestion of nutrition compared with dose 10% but was still higher than control. In conclusion, the supplementation of Leucaena leucocephala on completed feed based on ammoniated rice straw in vitro can increase nutrient degradability. Supplementation of 10% and 20% Leucaena leucochepala were suitable to be used for further studies, therefore in vivo experiment is required to study the effects on animal production.

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