The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS

Ferreira, Juliana Ferrari; Santana, José Carlos Curvelo; Tambourgi, Elias Basile

doi:10.1590/s1516-89132011000100017

Cited by 43 publications

(37 citation statements)

References 27 publications

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“…Ferreira et al, [18] noted that the ideal temperature range for the characterization of bromelain from pineapple stem bark is between 30°C and 40°C, which supports the similar behaviour observed in this study between bromelain from the different pineapple varieties. These results are also comparable to the characteristics of pineaple bromelain, which is active between 40ºC and 60ºC [19].…”

Section: Figure 3 Effect Of Temperature On Bromelain Activity Obtainsupporting

confidence: 90%

Characterization of Bromelain from Parts of Three Different Pineapple Varieties in Nigeria

Omotoyinbo¹,

Sanni²

2017

AJBIO

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Abstract:Bromelain was extracted from crown, flesh, core, and peel of some variant of Nigerian pineapple fruits in order to evaluate its amount and characteristics. Each part of pineapple after separation was weighed, blended, filtered and then filtrate was precipitated with ethanol and centrifuged. All extracts from each stage were collected and assayed for bromelain activity. The concentration of protein was also measured by using BSA as standard while proteolytic activity was determined using Azocasein 1% (w/v) as substrate at standard conditions. The optimum temperature and pH were also evaluated. The result showed that bromelain could be obtained from all parts of pineapple. Ananas fitzmulleri (Agric) had the highest weight (1486.42 g) and flesh content (53.5%) compared to other varieties while bromelain obtained from all parts and in the different varieties had optimum activity at 40°C and pH of 7. Hence ethanol precipitation method is viable for bromelain recovery.

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Section: Figure 3 Effect Of Temperature On Bromelain Activity Obtainsupporting

confidence: 90%

Characterization of Bromelain from Parts of Three Different Pineapple Varieties in Nigeria

Omotoyinbo¹,

Sanni²

2017

AJBIO

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“…In order to optimize the separation of bromelain response surface methodology has been worked in ATPS that yielded 90.33% enzyme and its purification factor was 2.4 (Navapara et al 2011). When thermodynamic equilibrium and ATPS were employed together for the purification of bromelain, purification fold came out to be among 25-62 (Ferreira et al 2011). Moreover, thermoseparation of bromelain resulted in active recovery of 79.5% with 1.25 purification fold (Rabelo et al 2004).…”

Section: Aqueous Two Phase System (Atps)mentioning

confidence: 99%

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Manzoor

Nawaz

Mukhtar

et al. 2016

Braz. arch. biol. technol.

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“…A considerable amount of literature has been published on the purification of bromelain using aqueous two-phase systems. Bromelain has been extracted from pineapple fruit by ATPS containing PEGpotassium phosphate (Babu et al 2008;Hebbar et al 2012) or PEG-phosphate buffer (Ferreira et al 2011). Bromelain has also been extracted from pineapple wastes by ATPS.…”

Section: Aqueous Two-phase System (Atps)mentioning

confidence: 99%

Bromelain: an overview of industrial application and purification strategies

Arshad

Amid

Yusof

et al. 2014

Appl Microbiol Biotechnol

160

134

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This review highlights the use of bromelain in various applications with up-to-date literature on the purification of bromelain from pineapple fruit and waste such as peel, core, crown, and leaves. Bromelain, a cysteine protease, has been exploited commercially in many applications in the food, beverage, tenderization, cosmetic, pharmaceutical, and textile industries. Researchers worldwide have been directing their interest to purification strategies by applying conventional and modern approaches, such as manipulating the pH, affinity, hydrophobicity, and temperature conditions in accord with the unique properties of bromelain. The amount of downstream processing will depend on its intended application in industries. The breakthrough of recombinant DNA technology has facilitated the large-scale production and purification of recombinant bromelain for novel applications in the future.

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The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS

Abstract: In this work, the thermodynamic equilibrium and applying of PEG4000/

Cited by 43 publications

References 27 publications

Characterization of Bromelain from Parts of Three Different Pineapple Varieties in Nigeria

Characterization of Bromelain from Parts of Three Different Pineapple Varieties in Nigeria

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Bromelain: an overview of industrial application and purification strategies

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