Bromelain: an overview of industrial application and purification strategies

Arshad, Zatul Iffah Mohd; Amid, Azura; Yusof, Faridah; Jaswir, Irwandi; Ahmad, Khaliq; Show, Pau Loke

doi:10.1007/s00253-014-5889-y

Cited by 163 publications

(153 citation statements)

References 107 publications

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“…There is also a limited pool of viable microorganisms, and strains with large nitrogen and phosphorus requirements cannot be used. Agro-industrial wastes that have been utilized include banana peel (Max et al 2010), cotton waste (Kiel et al 1981), kiwi fruit peel (Hang et al 1987), date pulp (Assadi & Nikkhah 2002), apple pomace ultrafiltration sludge and solid waste (Kareem et al 2010), orange peel waste (Torrado et al 2011), pineapple waste (Kareem et al 2010), wheat bran and soya bean meal (Sauer et al 2008), fresh kumara (Ipomoea batatas), potato (Solanum tuberosum) and taro (Colacasia esculenta) (Arshad et al 2014). …”

Section: Solid-state Fermentationmentioning

confidence: 99%

Overview of citric acid production from Aspergillus niger

Show

Oladele

Siew

et al. 2015

Frontiers in Life Science

215

126

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Citric acid has high economic potential owing to its numerous applications. It is mostly produced by microbial fermentation using Aspergillus niger. In view of surges in demand and growing markets, there is always a need for the discovery and development of better production techniques and solutions to improve production yields and the efficiency of product recovery. To support the enormous scale of production, it is necessary and important for the production process to be environmentally friendly by utilizing readily available and inexpensive agro-industrial waste products, while maintaining high production yields. This article reviews the biochemistry of citric acid formation, choices of citric-acid producing microorganisms and raw materials, fermentation strategies, the effects of various fermentation conditions, citric acid recovery options and the numerous applications of citric acid, based on information drawn from the literature over the past 10 years.

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Section: Solid-state Fermentationmentioning

confidence: 99%

Overview of citric acid production from Aspergillus niger

Show

Oladele

Siew

et al. 2015

Frontiers in Life Science

215

126

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“…Researchers thereby are trying various conventional as well as latest purification techniques to achieve bromelain in highest purified form at reduced cost (Arshad et al 2014). As compared to fruit, bromelain concentration is high in stem and is thus a cheaply available source of bromelain (Tochi et al 2008).…”

Section: Extraction and Purification Methodsmentioning

confidence: 99%

“…It is being used in food and beverage industries (Neta et al 2012), in meat tenderization, in cosmetic industries (Orsini 2006) as well as in textile industries (Arshad et al 2014). Besides its industrial applications, bromelain possess multiple therapeutic actions (Summarized in Table no.…”

Section: Applications Of Bromelainmentioning

confidence: 99%

“…Besides clinical approach, bromelain has also employed in various industries including food industries (Maurer 2001), such as breweries (Soares et al 2012) and flesh processing and tenderization, textile and cosmetic industries (Babu et al 2008;Ketnawal et al 2009). With the advent of recombinant DNA technology, scientists and researchers across the globe have been working on recombinant bromelain to achieve exaggerate and novel applications in future (Arshad et al 2014). …”

Section: Introductionmentioning

confidence: 99%

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Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Manzoor

Nawaz

Mukhtar

et al. 2016

Braz. arch. biol. technol.

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“…Bromelain is notable for being used in multitude industrial applications such as meat tenderization, food, beverages, and pharmaceuticals, hence increases its market demand [2]. However, there is a rising argument whether pineapple…”

Section: Introductionmentioning

confidence: 99%

COMPARISON OF PURIFICATION METHODS TO PURIFY RECOMBINANT BROMELAIN FROM Escherichia coli BL21-A1

Arshad¹,

Amid²,

Yusof³

et al. 2017

MJAS

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Recombinant bromelain is a cysteine protease that can be exploited for its protease activity in food and pharmaceutical applications. The aim of this study was to compare different purification methods aqueous two-phase system (ATPS), ammonium sulphate precipitation, ion exchange, affinity, and gel filtration chromatography) for the recombinant bromelain purification from Escherichia coli BL21-A1. From the SDS-PAGE analysis, all methods produced band with molecular weight between 50 to 55 kDa. Among the methods used, the ATPS purification consisting of 13% (w/w) of PEG6000 and 11% (w/w) potassium phosphate at pH 7.0 was chosen as the best purification method. The method produced 16.39 ± 0.03 % of yield, purification fold of 5.35 ± 0.11, and specific activity of 3.47 ± 0.11 unit/mg of recombinant bromelain. This proposed study can be used as a platform for large-scale downstream processing of recombinant bromelain in industry.Keywords: recombinant bromelain, purification, chromatography, aqueous two-phase system Abstrak Rekombinan bromelain adalah sisteina protease yang boleh dieksploitasi dalam aplikasi makanan dan farmaseutikal. Tujuan kajian ini adalah untuk membandingkan kaedah penulenan yang berbeza dengan menggunakan sistem akueus dua fasa (ATPS), pemendakan amonium sulfat, pertukaran ion, afiniti dan gel penapisan kromatografi untuk penulenan rekombinan bromelain daripada Escherichia coli BL21-A1. Daripada analisis SDS-PAGE, semua kaedah menghasilkan jalur dengan berat molekul antara 50 hingga 55 kDa. Antara kaedah yang digunakan, sistem akueus dua fasa (ATPS) yang terdiri daripada 13% (w/w) PEG6000 dan 11% (w/w) kalium fosfat pada pH 7.0 telah dipilih sebagai kaedah penulenan yang terbaik. Kaedah ini mendapat 16.39 ± 0.03% hasil, kadar penulenan 5.35 ± 0.11, dan aktiviti rekombinan bromelain sebanyak 3.47 ± 0.11 unit/mg. Kajian ini dicadangkan boleh digunakan sebagai platform untuk pemprosesan hiliran rekombinan bromelain secara besar-besaran dalam industri.Kata kunci: rekombinan bromelain, penulenan, kromatografi, sistem akueus dua fasa IntroductionBromelain is a plant protease that is commercially obtained from stem, fruit, and waste of pineapple plant [1]. Bromelain is notable for being used in multitude industrial applications such as meat tenderization, food, beverages, and pharmaceuticals, hence increases its market demand [2]. However, there is a rising argument whether pineapple

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Bromelain: an overview of industrial application and purification strategies

Cited by 163 publications

References 107 publications

Overview of citric acid production from Aspergillus niger

Overview of citric acid production from Aspergillus niger

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

COMPARISON OF PURIFICATION METHODS TO PURIFY RECOMBINANT BROMELAIN FROM Escherichia coli BL21-A1

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