The Effect of Layer-by-Layer Assembly Coating on the Proliferation and Differentiation of Neural Stem Cells

Abstract

Nanocoating of a single-cell with biocompatible materials creates a defined microenvironment for cell differentiation and proliferation, as well as a model for studies in cell biology. In addition, the acidic environment in the tissue of stroke victims necessitates drug release upon pH stimuli. Here, we report the encapsulation of single neural stem cells (NSCs) using a layer-by-layer (LbL) self-assembly technique with polyelectrolytes gelatin and alginate. Analysis of the NSCs showed that the LbL encapsulation would not affect the viability, proliferation, or differentiation of the cells. When insulin-like growth factor-1 (IGF-1) was loaded on the coating material alginate, its release from alginate into the medium presented in a time-dependent and pH-dependent way. IGF-1 significantly enhanced the proliferation of the encapsulated NSCs, demonstrating a drug-carrier function of the LbL single-cell nanocoating. It provided a potential treatment strategy for nervous system disorders such as stroke.

Traceable Nanoparticle Delivery of Small Interfering RNA and Retinoic Acid with Temporally Release Ability to Control Neural Stem Cell Differentiation for Alzheimer's Disease Therapy

“…Alzheimer's disease (AD) is the most prevalent age‐related neurodegenerative disorder . The predominant pathologies of AD are gliosis and widespread neuronal loss, which progressively cause impairments in the memory and cognitive functions . Current therapies, such as treatment with acetylcholinesterase inhibitors to enhance the cholinergic function, are inefficient due to the great hindrance at the blood–brain barrier (BBB) and their poor target ability to the diseased sites .…”

Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

“…Layer‐by‐layer (LbL) self‐assembly has emerged as an approach in cell surface engineering . Multiple cells in each microgel with excessive gel disposition on cell surface may be detrimental to the fragile cells .…”

“…Moreover, it was also studied for the encapsulation of bacteria and fungi to protect and maintain the metabolic activity . Recently, LbL self‐assembly has reportedly been used for tumor and neural cells to provide a biological microenvironment, which exhibited great potential for many disease treatment …”

Metal–Organic Framework Coatings as Cytoprotective Exoskeletons for Living Cells

“…Artificially induced cell hibernation engenders extended cell lifetimes and therefore underpins the advancement of engineering strategies toward governing cell division, proliferation, and differentiation . Thus, we were motivated to investigate the time‐dependent cellular responses of ZIF‐8 coated yeast cells.…”