Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Wang, Jin; Miao, Yong; Huang, Yong; Lin, Bojie; Liu, Xiaomin; Xiao, Shan; Du, Lijuan; Hu, Zhiqi; Xing, Malcolm

doi:10.1002/adhm.201700447

Cited by 42 publications

(34 citation statements)

References 53 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo experiment by nude mice showed that this nanogel–cell complex exhibited no toxicity, and hair shafts were induced in 4 weeks. [ 112 ] Problems of immune response has also been solved using material integration strategy. Pluripotent stem cells (PSCs) are capable for treating myocardial infarction (MI); however, with low concentration and short retention time, the effects cannot be guaranteed.…”

Section: Biomedical Applications Of Materials Integrated Organismsmentioning

confidence: 99%

Organism–Materials Integration: A Promising Strategy for Biomedical Applications

Cui

Wang

et al. 2020

Advanced NanoBiomed Research

View full text Add to dashboard Cite

In nature, organisms play an essential role in harnessing elements to produce materials. Being precisely integrated with the biological structures, the materials confer organisms with various unique functions such as protection, recognition guiding, biocatalysis, etc. Inspired by this phenomenon, elaborately designed materials can be grafted to different organisms such as cells, eukaryotes, and viruses via artificial incorporation strategies. Herein, progresses upon the methods and techniques of organism–materials integration are discussed, including spontaneous formation, artificial enhancement, and genetic engineering. The integration of organism and materials can alter the biological behavior and even offer the organism rationally designed functions, facilitating the biological applications of organisms in the field such as vaccine improvement, biomedical therapy, and biomedical imaging. These unique effects achieved by the combination of organisms and materials propose a new strategy for providing precise control over organisms. These promising strategies also offer new perspectives of biology and chemistry development, and show great potential in future biomedical therapy.

show abstract

Section: Biomedical Applications Of Materials Integrated Organismsmentioning

confidence: 99%

Organism–Materials Integration: A Promising Strategy for Biomedical Applications

Cui

Wang

et al. 2020

Advanced NanoBiomed Research

View full text Add to dashboard Cite

show abstract

“…C) Schematic illustration of the construction of dermal papilla spheroids by aggregation of LbL encapsulated dermal papilla cells for hair follicle regeneration. Adapted with permission . Copyright 2017, Wiley‐VCH.…”

Section: Biomedical Applications Of Lbl Self‐assembly For Cell Encapsmentioning

confidence: 99%

“…Apart from constructing biomimetic tissues by LbL encapsulating cell with nanometer‐sized fibronectin–gelatin films, biomimetic tissues can also be prepared by physical cross‐linking on LbL encapsulated cells to form the cell spheroids . For example, our group previously reported the fabrication of dermal papilla spheroids for hair follicle regeneration, as shown in Figure C. Individual dermal papilla cells were encapsulated with gelatin and alginate by LbL assembly to form ultrathin nanocoated matrices.…”

Section: Biomedical Applications Of Lbl Self‐assembly For Cell Encapsmentioning

confidence: 99%

Biomedical Applications of Layer‐by‐Layer Self‐Assembly for Cell Encapsulation: Current Status and Future Perspectives

Liu

Wang

Zhong

et al. 2018

Adv Healthcare Materials

Self Cite

111

View full text Add to dashboard Cite

Encapsulating living cells within multilayer functional shells is a crucial extension of cellular functions and a further development of cell surface engineering. In the last decade, cell encapsulation has been widely utilized in many cutting‐edge biomedical fields. Compared with other techniques for cell encapsulation, layer‐by‐layer (LbL) self‐assembly technology, due to the versatility and tunability to fabricate diverse multilayer shells with controllable compositions and structures, is considered as a promising approach for cell encapsulation. This review summarizes the state‐of‐the‐art and potential future biomedical applications of LbL cell encapsulation. First of all, a brief introduction to the LbL self‐assembly technique, including assembly mechanisms and technologies, is made. Next, different cell encapsulation strategies by LbL self‐assembly techniques are explained. Then, the biomedical applications of LbL cell encapsulation in cell‐based biosensors, cell transplantation, cell/molecule delivery, and tissue engineering, are highlighted. Finally, discussions on the current limitations and future perspectives of LbL cell encapsulation are also provided.

show abstract

“…For instance, alginate is a natural polysaccharide derived from algae, and gelatin is a protein derivative. Owing to their biocompatibility and biodegradability, these substances have been widely used in ECM tissue engineering 28 - 32 . In the current study, gelatin and alginate were used to coat HFSCs in the engineering of nanoscale biomimetic ECM for individual cells.…”

Section: Introductionmentioning

confidence: 99%

Nanoscale microenvironment engineering based on layer-by-layer self-assembly to regulate hair follicle stem cell fate for regenerative medicine

et al. 2020

Self Cite

View full text Add to dashboard Cite

Hair regenerative medicine, a promising strategy for the treatment of hair loss, will likely involve the transplantation of autologous hair follicular stem cells (HFSCs) and dermal papilla cells (DPCs) into regions of hair loss. Cyclic hair regeneration results from the periodic partial activation of HFSCs. However, previous studies have not successfully achieved large-scale HFSC expansion in vitro without the use of feeder cells , with a lack of research focused on regulating HFSC fate for hair follicular (HF) regeneration. Hence, an emerging focus in regenerative medicine is the reconstruction of natural extracellular matrix (ECM) regulatory characteristics using biomaterials to generate cellular microenvironments for expanding stem cells and directing their fate for tissue regeneration. Methods: HFSCs were coated with gelatin and alginate using layer-by-layer (LbL) self-assembly technology to construct biomimetic ECM for HFSCs; after which transforming growth factor (TGF)-β2 was loaded into the coating layer, which served as a sustained-release signal molecule to regulate the fate of HFSCs both in vitro and in vivo . In vitro experiments (cell culture and siRNA) were employed to investigate the molecular mechanisms involved and in vivo implantation was carried out to evaluate hair induction efficiency. Results: Nanoscale biomimetic ECM was constructed for individual HFSCs, which allowed for the stable amplification of HFSCs and maintenance of their stem cell properties. TGF-β2 loading into the coating layer induced transformation of CD34 + stem cells into highly proliferating Lgr5 + stem cells, similar to the partial activation of HFSCs in HF regeneration. Thus, LbL coating and TGF-β2 loading partially reconstructed the quiescent and activated states, respectively, of stem cells during HF regeneration, thereby mimicking the microenvironment that regulates stem cell fate for tissue regeneration during HF cycling. Improved HF regeneration was achieved when the two HFSC states were co-transplanted with neonatal mouse dermal cells into nude mice. Conclusion: This study provides novel methods for the construction of stem cell microenvironments and experimental models of HF regeneration for the treatment of hair loss.

show abstract

Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Cited by 42 publications

References 53 publications

Organism–Materials Integration: A Promising Strategy for Biomedical Applications

Organism–Materials Integration: A Promising Strategy for Biomedical Applications

Biomedical Applications of Layer‐by‐Layer Self‐Assembly for Cell Encapsulation: Current Status and Future Perspectives

Nanoscale microenvironment engineering based on layer-by-layer self-assembly to regulate hair follicle stem cell fate for regenerative medicine

Contact Info

Product

Resources

About