The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation

Amirian, Jhaleh; Linh, Nguyen Thuy Ba; Min, Young Ki; Lee, Byong‐Taek

doi:10.1002/app.41241

Cited by 20 publications

(20 citation statements)

References 52 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sustained release may be attributed to the electrical interaction between the positively charged amino acid residues in the VEGF proteins, and the negatively charged sites of the pectin, FG. The release profi les of VEGF from FG also confi rmed the strong interaction which was similar with previous study 45) . The results indicated that the VEGF protein was released from the fi brin clots more slowly, and this fi nding was in agreement with previous studies using diff erent types of delivery system 46,47) .…”

Section: Discussionsupporting

confidence: 90%

Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part II

Enezei

Afsari

Takeuchi

et al. 2018

J. Hard Tissue Biology.

View full text Add to dashboard Cite

In bone tissue engineering, angiogenesis is closely associated with osteogenesis where reciprocal interactions between endothelial and osteoblast cells play an important role in bone regeneration. Over-expression of the angiogenesisrelated gene due to a higher dose of vascular endothelial growth factor (VEGF) protein can inhibit osteogenesis process at mRNA level. To study the eff ect of controlled released of the VEGF protein incorporating fi brin glue (FG) treated with fabricated porous biphasic calcium phosphate (BCP) on osteogenesis gene (BMP-2) and angiogenesis gene (VEGF) on dental stem cells (DSCs) at mRNA level. DSCs were treated with two diff erent modalities; VEGF protein incorporated FG, and VEGF protein incorporated FG added-BCP treated media. The cells were harvested at four diff erent time intervals (day 3, day 7, day 10 and day 14) and were subjected to RNA isolation using the RNA extraction kit. This was followed by performing one step-reverse transcriptase-PCR (RT-PCR) to amplify the osteogenesis BMP-2 gene, angiogenesis VEGF gene and the osteoblast-specifi c transcription factor expression Osterix (Osx) with and without the controlled release of VEGF protein. The RT-PCR products were then electrophoresed. The gel image was captured using Image Analyser. Controlled release of VEGF protein using FG as a natural delivery system, using a single growth factor, show a signifi cantly enhanced osteogenesis BMP-2 gene and angiogenesis VEGF gene with a high expression of Osx compare with non-delivered free VEGF protein treated groups. FG is a biocompatible material that could be employed as a delivery vehicle for controlled release of VEGF protein single or dual release in bone tissue engineering strategy and design of the study. Application of this method for using FG is mixing with a porous ceramic scaff old loading with the growth factors is a convenient and promising strategy for improving osteogenesis and angiogenesis processes of reconstruction critical-sized bone defects and might change the scope of modern surgery.

show abstract

Section: Discussionsupporting

confidence: 90%

Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part II

Enezei

Afsari

Takeuchi

et al. 2018

J. Hard Tissue Biology.

View full text Add to dashboard Cite

show abstract

“…Jung et al . (2013) have demonstrated that BMP‐2 loaded in Col exhibits the best release with almost 70% of BMP‐2 being released during the 2nd day . Brown et al .…”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that different types of polymer and ceramics used in composite scaffold have different drug or growth factor release profiles . In fact, incorporation of some bioceramic nanopowder such as HAp and BCP into polymeric matrix can increase drug integrity result in the sustain release of immobilized or incorporated drug from composite scaffold . In addition to, the sustain release in the polymeric and composite system can attributed to positive charge of BMP‐2 amino acid and negative charge of polymer(negative charge) and bioceramics such as BCP and HAp .…”

Section: Resultsmentioning

confidence: 99%

“…In fact, incorporation of some bioceramic nanopowder such as HAp and BCP into polymeric matrix can increase drug integrity result in the sustain release of immobilized or incorporated drug from composite scaffold . In addition to, the sustain release in the polymeric and composite system can attributed to positive charge of BMP‐2 amino acid and negative charge of polymer(negative charge) and bioceramics such as BCP and HAp . To date, different types of biomaterials such as Col, hyaluronic acid, dextran, chitosan, PLGA, and fibrion have been applied for BMP‐2 delivery.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Bone morphogenetic protein‐2 immobilization on porous PCL‐BCP‐Col composite scaffolds for bone tissue engineering

Song

Amirian

Linh

et al. 2017

J of Applied Polymer Sci

Self Cite

View full text Add to dashboard Cite

The objective of this study was to develop novel porous composite scaffolds for bone tissue engineering through surface modification of polycaprolactone-biphasic calcium phosphate-based composites (PCL-BCP). PCL-BCP composites were first fabricated with salt-leaching method followed by aminolysis. Layer by layer (LBL) technique was then used to immobilize collagen (Col) and bone morphogenetic protein (BMP-2) on PCL-BCP scaffolds to develop PCL-BCP-Col-BMP-2 composite scaffold. The morphology of the composite was examined by scanning electron microscopy (SEM). The efficiency of grafting of Col and BMP-2 on composite scaffold was measured by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). Both XPS and FTIR confirmed that Col and BMP-2 were successfully immobilized into PCL-BCP composites. MC3TC3-E1 preosteoblasts cells were cultivated on composites to determine the effect of Col and BMP-2 immobilization on cell viability and proliferation. PCL-BCP-Col-BMP-2 showed more cell attachment, cell viability, and proliferation bone factors compared to PCL-BCP-Col composites. In addition, in vivo bone formation study using rat models showed that PCL-BCP-Col-BMP-2 composites had better bone formation than PCL-BCP-Col scaffold in critical size defect with 4 weeks of duration. These results suggest that PCL-BCP-Col-BMP-2 composites can enhance bone regeneration in critical size defect in a rat model with 4 weeks of duration.

show abstract

“…Gelatin is derived from high molecular weight collagen by breaking the natural triple-helix structure of collagen into single-stranded molecules; it has been used in many aspects of tissue engineering because of its biocompatibility and ease of gelation [18]. Due to the loosened structure of the biopolymer skeleton, gelatin can easily spread across and over the scaffold surface.…”

Section: Introductionmentioning

confidence: 99%

Gelatin Tight-Coated Poly(lactide-co-glycolide) Scaffold Incorporating rhBMP-2 for Bone Tissue Engineering

Wang

Li³

et al. 2015

Materials

View full text Add to dashboard Cite

Surface coating is the simplest surface modification. However, bioactive molecules can not spread well on the commonly used polylactone-type skeletons; thus, the surface coatings of biomolecules are typically unstable due to the weak interaction between the polymer and the bioactive molecules. In this study, a special type of poly(lactide-co-glycolide) (PLGA)-based scaffold with a loosened skeleton was fabricated by phase separation, which allowed gelatin molecules to more readily diffuse throughout the structure. In this application, gelatin modified both the internal substrate and external surface. After cross-linking with glutaraldehyde, the surface layer gelatin was tightly bound to the diffused gelatin, thereby preventing the surface layer gelatin coating from falling off within 14 days. After gelatin modification, PLGA scaffold demonstrated enhanced hydrophilicity and improved mechanical properties (i.e., increased compression strength and elastic modulus) in dry and wet states. Furthermore, a sustained release profile of recombinant human bone morphogenetic protein-2 (rhBMP-2) was achieved in the coated scaffold. The coated scaffold also supported the in vitro attachment, proliferation, and osteogenesis of rabbit bone mesenchymal stem cells (BMSCs), indicating the bioactivity of rhBMP-2. These results collectively demonstrate that the cross-linked-gelatin-coated porous PLGA scaffold incorporating bioactive molecules is a promising candidate for bone tissue regeneration.

show abstract

The effect of BMP‐2 and VEGF loading of gelatin‐pectin‐BCP scaffolds to enhance osteoblast proliferation

Cited by 20 publications

References 52 publications

Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part II

Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part II

Bone morphogenetic protein‐2 immobilization on porous PCL‐BCP‐Col composite scaffolds for bone tissue engineering

Gelatin Tight-Coated Poly(lactide-co-glycolide) Scaffold Incorporating rhBMP-2 for Bone Tissue Engineering

Contact Info

Product

Resources

About