Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part II

Abstract

In bone tissue engineering, angiogenesis is closely associated with osteogenesis where reciprocal interactions between endothelial and osteoblast cells play an important role in bone regeneration. Over-expression of the angiogenesisrelated gene due to a higher dose of vascular endothelial growth factor (VEGF) protein can inhibit osteogenesis process at mRNA level. To study the eff ect of controlled released of the VEGF protein incorporating fi brin glue (FG) treated with fabricated porous biphasic calcium phosphate (BCP) on osteogenesis gene (BMP-2) and angiogenesis gene (VEGF) on dental stem cells (DSCs) at mRNA level. DSCs were treated with two diff erent modalities; VEGF protein incorporated FG, and VEGF protein incorporated FG added-BCP treated media. The cells were harvested at four diff erent time intervals (day 3, day 7, day 10 and day 14) and were subjected to RNA isolation using the RNA extraction kit. This was followed by performing one step-reverse transcriptase-PCR (RT-PCR) to amplify the osteogenesis BMP-2 gene, angiogenesis VEGF gene and the osteoblast-specifi c transcription factor expression Osterix (Osx) with and without the controlled release of VEGF protein. The RT-PCR products were then electrophoresed. The gel image was captured using Image Analyser. Controlled release of VEGF protein using FG as a natural delivery system, using a single growth factor, show a signifi cantly enhanced osteogenesis BMP-2 gene and angiogenesis VEGF gene with a high expression of Osx compare with non-delivered free VEGF protein treated groups. FG is a biocompatible material that could be employed as a delivery vehicle for controlled release of VEGF protein single or dual release in bone tissue engineering strategy and design of the study. Application of this method for using FG is mixing with a porous ceramic scaff old loading with the growth factors is a convenient and promising strategy for improving osteogenesis and angiogenesis processes of reconstruction critical-sized bone defects and might change the scope of modern surgery.

Design and applications of protein delivery systems in nanomedicine and tissue engineering

“…For example, Huber et al investigated the possibility to use commercial demineralized bone matrix (DBM) putty as BMP2 delivery system to enhance the osteinductive ability of the DBM [199]. In another study, Enezei et al compared the BMP2 and VEGF expression in dental stem cells after treatment with VEGF-loaded fibrin glue or VEGF-loaded fibrin glue treated with porous biphasic calcium phosphate [200].…”

The Effect of Strontium on Osteoblastogenesis and Osteoclastogenesis in Dental Stem Cells-induced Epidermal Growth Factor at Molecular Level: <i>In Vitro</i> Study

“…Aprotinin solution was used after diluting it from 3,000 KIU/ml to 100 KIU/ml. EGF-specific enzyme-linked immunosorbent assay (ELISA) kit was used to measure the potency and constant release of EGF from FG matrix 21) . To validate the analytical procedure and statistical analysis, the tests were run in triplicate (Fig.…”

Healing of induced tongue defects using erythropoietin hydrogel (an experimental study on rats)

“…Treatment of oral and maxillofacial diseases could possibly include antibiotics, analgesics [ 5 ], anti-inflammatories, angiogenic factors [ 6 ], herbal medicines [ 7 ], and specific regional treatments, such as chemical cauterization, surgical removal, and laser therapy [ 8 ]. However, these treatment may not cause significant improvement or can even result in several unexpected side effects [ 9 ].…”