The effect of bee propolis on oral pathogens and human gingival fibroblasts

Sönmez, Şule; Kirilmaz, Levent; Yücesoy, Mine; Yücel, Banu; Yılmaz, Banu

doi:10.1016/j.jep.2005.06.035

Cited by 91 publications

(73 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The use of propolis in test sites showed significant reduction in the prevalence of the three pathogens. Some in vitro and in vivo studies have demonstrated activity of propolis against periodontal pathogens [7,17,[21][22][23][24][25][26][27][28]. Mechanisms of activity of propolis against microorganisms are still controversial.…”

Section: Discussionmentioning

confidence: 99%

Health from the Hive: Propolis as an Adjuvant in the Treatment of Chronic Periodontitis - A Clinicomicrobiologic Study

2014

JCDR

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Health from the Hive: Propolis as an Adjuvant in the Treatment of Chronic Periodontitis - A Clinicomicrobiologic Study

2014

JCDR

View full text Add to dashboard Cite

show abstract

“…Many studies have examined propolis antimicrobial activity using serial dilution tests (Sonmez et al, 2005;Victorino et al, 2007) and diffusion tests on agar (Kujumgiev et al, 1999;Somnes et al,. 2005;Bruschi et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial activity of propolis-based toothpastes for endodontic treatment

Victorino

Bramante

Watanabe

et al. 2009

Braz. J. Pharm. Sci.

View full text Add to dashboard Cite

This study evaluated the antibacterial activity of propolis-based toothpastes used as intracanal medication in endodontic treatment. The propolis-based toothpastes were prepared using an extract established in previous studies (identified as A70D and D70D). Calcium hydroxide paste was used as a control. The diffusion-well method on double-layer agar was used in a culture medium of Tryptic Soy Agar. The plates were kept at room temperature for two hours to allow the diffusion of pastes in the culture medium, and then incubated at 35º C for twenty-four hours in aerobiosis and in microaerophilia (S. mutans). After this period, the total diameter of the inhibition halo was measured. The results were analyzed by ANOVA analysis of variance, followed by the Tukey test at p<0.05. The propolis-based toothpastes presented antibacterial activity against 83.3% of the analyzed bacteria. For 66.7% of these bacteria, the propolis-based toothpastes exhibited greater antibacterial activity than calcium hydroxide. The present results allow us to conclude that the experimental pastes A70D and D70D showed good activity against aerobic bacteria, proving more effective than calcium hydroxide.Uniterms: Propolis/antibacterial activity. Tooth paste/with propolis/antibacterial activity. Endodontics/ treatment.O objetivo deste estudo foi avaliar a atividade antibacteriana de formas farmacêuticas a base de própolis para uso no tratamento endodôntico como medicação intracanal. As formulações de própolis, em forma de pastas, foram preparadas a partir de um extrato pré-estabelecido em estudos anteriores e identificadas como A70D e D70D. Como controle, foi utilizado pasta de hidróxido de cálcio. (26.5 -coagulase negativa) e Staphylococcus epidermidis (6epi). Foi utilizado o método poço difusão em camada dupla de ágar, em meio de cultura Tryptic Soy Agar. As placas foram mantidas à temperatura ambiente por 2 h para permitir a difusão das pastas no meio de cultura, e então incubadas a 35 ºC por 24 h em aerobiose e em microaerofilia (S. mutans). Após este período, foi medido o diâmetro total do halo de inibição. Os resultados foram submetidos ao teste de análise de variância ANOVA seguido do teste de Tukey com p<0,05. As pastas a base de própolis apresentaram atividade antibacteriana contra 83,3% das bactérias analisadas. Para 66,7% das bactérias, as pastas de própolis apresentaram maior atividade

show abstract

“…This protocol was in accordance to the requirements issued by the Research Ethics Committee of Universiti Kebangsaan Malaysia (UKM1.5.3.5/244/NF-04-09) and modified from previous reported studies [11,12]. Gingival tissues were scrapped off from the tooth surface using sterile blade and cultured in 6-well plates in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% foetal bovine serum (FBS), 1% Penicillin-Streptomycin and 1% fungizone (Amphotericin B).…”

Section: Human Gingival Fibroblast (Hgf) Culturementioning

confidence: 99%

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

Said¹

2013

TOPROCJ

View full text Add to dashboard Cite

Abstract:The aims of the study were to determine the in vitro cytotoxicity and anti-inflammatory activities of common edible herbs and spices on cultured human gingival fibroblasts (HGFs). Piper betle L. (betel leaf), P.sarmentosum Roxb.(wild betel / kadok leaf), P.nigrum L. (black pepper seed), Eugenia caryophyllata L. (clove bud) and Cinnamomum zeylanicum Blume (cinnamon bark). Essential oils were extracted using steam distillation technique and analysed using gas chromatography (GC) and gas chromatography -mass spectrometry (GC-MS). The HGFs were exposed to essential oils at 5 -0.04 µg/mL in less than 1% dimethylsulfoxide and the number of viable cells was counted to assess cytotoxicity effect. Anti-inflammatory action was determined via the inhibitory action of Interleukin-6 (IL-6), a major proinflammatory cytokine in the periodontal tissue inflammation. Treatment of fibroblasts with essential oils resulted in > 70% cell viability. The oils from black pepper seed, clove bud and cinnamon bark showed dose-dependent inhibitory action on IL-6 on cultured bacterial Lipopolysaccharide (LPS)-induced human gingival fibroblasts. Of all the oils, cinnamon bark oil showed the most prominent action comparable to acetylsalicylic acid. Conclusion: Essential oils of selected herbs and spices retained compatibility with gingival fibroblasts in culture and showed inhibitory activity on IL-6 released by LPS-induced HGFs. These findings suggest therapeutic potential for application of assay in the management of periodontal disease.

show abstract

The effect of bee propolis on oral pathogens and human gingival fibroblasts

Cited by 91 publications

References 25 publications

Health from the Hive: Propolis as an Adjuvant in the Treatment of Chronic Periodontitis - A Clinicomicrobiologic Study

Health from the Hive: Propolis as an Adjuvant in the Treatment of Chronic Periodontitis - A Clinicomicrobiologic Study

Antibacterial activity of propolis-based toothpastes for endodontic treatment

Anti-Inflammatory Activity of Selected Edible Herbs and Spices on Cultured Human Gingival Fibroblasts

Contact Info

Product

Resources

About