The diversity of Nepenthes at the post-mining area in Sintang District, West Kalimantan, Indonesia

Abstract

Pitoyo A, Prameta AA, Marsusi, Suratman, Suranto. 2018. Morphological, anatomical and isozyme variability among taro (Colocasia esculenta) accessions from southeastern part of Central Java, Indonesia. Biodiversitas 19: 1811-1819. The objective of this study was to evaluate morphological, anatomical and isozyme variability among taro accessions from southeastern part of Central Java (Indonesia). A total of 20 taro accessions were collected from a wide range of sites during field surveys. Morphological characters measurements were taken on vegetative structures such as roots, stems, leaves, and corms. Anatomical characters were observed from both paradermal and transverse sections of leaf. Identification of biochemical markers was done by using peroxidase and esterase isozyme system. A UPGMA dendrogram among accessions was constructed based on the genetic similarity matrix by applying a cluster analysis using a computer programme, NTSYS Version 2.00. The analysis of variance for morphological and anatomical characters revealed that there was significant difference for majority of the tested traits indicating that there was a variability among the taro accessions. Polymorphism was observed using isozymes of esterase (12 banding pattern) and peroxidase (8 banding pattern). Based on the dendrogram at a level of 62 % similarity, taro accessions were segregated into two major clusters. In Cluster I, the closest relationship was shown between SKH and SKA accessions that had 96 % coefficient of similarity. The ten accessions from Klaten, Sragen, and Karanganyar were then clustered separately as Cluster II with coefficient of similarity 73.52 %.

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

“…The activity of an enzyme will result in very specific isozyme banding patterns. Twelve (12) bands of esterase isozymes with Rf values ranged from 0.08 to 0.95 were detected by Pitoyo et al (2018) in taro (Colocasia esculenta) accessions from southeastern part of Central Java. Meanwhile 8 bands of peroxidase isozymes detected with Rf values from 0.08 to 0.13.…”

Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents

“…Morphological characters are still routinely used for preliminary evaluation because they are fast, simple, cheap and can be used as a general approach to assessing plant genetic diversity (Jingura and Kamusoko 2015). Pitoyo et al (2018) reported that taro accessions showed high variability in morphological characters. Morphological characters, therefore, can be used to distinguish the variability of varieties.…”

“…The thickness of the cuticle layer is influenced by the presence of cutin compounds which are able to prevent the evaporation of water molecules from the leaves (Ali et al 2009). Pitoyo et al (2018) reported significant differences for the majority the anatomical characters of taro indicating a high variability among the taro accessions.…”

“…Observation of anatomical characters was performed using a binocular microscope, Olympus CH-20. Measurement of anatomical characters was done using a calibrated ocular micrometer (Hammed et al 2013;Pitoyo et al 2018;Samiyarsih et al 2018).…”

“…Morphological dan anatomical characters have an important role in plant taxonomy and identification. The analysis of variance of morphological and anatomical characters revealed significant differences for majority of the tested traits, indicating there was variability among the taro accessions (Pitoyo et al 2018). Environmental conditions trigger various plant responses including changes in morphology, anatomy, gene expression, cell metabolism and growth and productivity (Anjum et al 2011).…”