Abstract. Samiyarsih S, Naipospos N, Palupi D. 2019. Variability of Catharanthus roseus based on morphological and anatomical characters, and chlorophyll contents. Biodiversitas 20: 2986-2993. Catharanthus roseus (L.) is an important medicinal plant of the family Apocynaceae used to treat many of the diseases. C. roseus also possess good antioxidant potential. The purpose of this research was 1) to determine the variability of C. roseus based on the morphological, anatomical characters and chlorophyll contents, 2) to know the differences in morphological, anatomical characteristics and chlorophyll contents of C. roseus found in Banyumas Regency. The research used a survey method, with a purposive sampling technique. Preparation of leaf for anatomical analysis was based on paraffin method. The results showed that C. roseus in Banyumas Regency were grouped into eight varieties. The morphological characters observed consisted of 21 characters, 8 quantitative characters, and 13 qualitative characters. Most of the quantitative morphological and anatomical characters were significantly diverse (p <0.05) except for petiole length, corolla number, stomata width and number of trichomata. The eight varieties of C. roseus have different chlorophyll a, chlorophyll b, and total chlorophyll contents but their anthocyanin contents were not significantly different.
Winged bean (Psophocarpus tetragonolobus (L.) DC) is an alternative food source that can be cultivated optimally. The application of mutation techniques on winged bean can provide genetic variations and have an essential role in the agricultural industry, especially in the assembly of superior cultivars. This research aimed to determine the anatomical response and chlorophyll content of winged plants undergoing mutations due to Cobalt 60 gamma-ray radiation and finding out the differences in the anatomical character between mutated and unmutated winged leaves. The experiments used a factorial, Completely Randomized Design (CRD). The factor I was winged plants and factor II was the concentration of Cobalt 60 radiation (0, 25, 50, 75, and 100 grays), each with 5 replicates. The results showed that the Cobalt 60 gamma-ray radiation increased the cuticle and leaf mesophyll thickness, but decreased the stomatal length and density of winged leaves. Cobalt 60 ray radiation affects the chlorophyll content of winged leaves. The total chlorophyll content has increased significantly, followed by an increase in the amount of gamma-ray radiation. The exposure radiation time of 40 minutes gives a response of high anatomical character and chlorophyll content than the exposure for 20 minutes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.