Abstract:Sopialena, Suyadi, Sahil M, Nurdiana J. 2018. The diversity of endophytic fungi associated with Piper nigrum in the tropical areas: a recent study from Kutai Kartanegara, Indonesia. Biodiversitas 19: 2028-2034. This research aimed to identify the diversity of endophytes fungal in the root and leaves of Piper nigrum Linn. This research contributes to the knowledge gaps by focusing the discussion on the Endophytic fungal communities of Piper nigrum Linn in tropical areas at Kutai Kartanegara, Indonesia. Two cert… Show more
“…Identification was carried out by observing morphological characteristics of colony growth, colony color, insulation on hyphae, color, and shape of spores and conidiophores. The books used to identify the colonies were: Barnett (1962), Barnett and Hunter (1998), Hanlin (1990Hanlin ( , 1998, and Seifert et al (2011).…”
Section: Fungal Isolate Purification and Characterizationmentioning
Abstract. Hidayah BN, Herawati N, Aisah AR, Utami NR. 2021. Diversity of fungi associated with rhizosphere of healthy and diseased garlic crop. Biodiversitas 22: 1433-1440. Garlic (Allium sativum L.) is an especially important horticultural crop in Indonesia because it is used as a cooking spice and for medicines and cosmetic ingredients. It is mostly grown in irrigated highlands; however Indonesian production is only supplying at most 17.5% of domestic demand. West Nusa Tenggara (WNT) Province is one of the largest garlic-producing regions in Indonesia. Currently, garlic cultivation in WNT Province is facing a problem of root rot disease. Therefore, it is necessary to investigate the fungi associated with garlic root rot disease. Research was conducted in June – September 2018 and samples were collected from Sembalun highlands (1200 m above sea level). Seventeen fungal isolates were collected from the rhizosphere of the garlic crops and fourteen of them were identified. The fungi isolated from the rhizosphere of healthy garlic crops were from the genus Aspergillus, Penicillium, and Paecilomyces. While the fungi isolated from the rhizosphere of diseased garlic crops were from the genus Aspergillus, Penicillium, Paecilomyces, Fusarium, and Phoma. The genus Fusarium and Phoma are well known as plant pathogens.
“…Identification was carried out by observing morphological characteristics of colony growth, colony color, insulation on hyphae, color, and shape of spores and conidiophores. The books used to identify the colonies were: Barnett (1962), Barnett and Hunter (1998), Hanlin (1990Hanlin ( , 1998, and Seifert et al (2011).…”
Section: Fungal Isolate Purification and Characterizationmentioning
Abstract. Hidayah BN, Herawati N, Aisah AR, Utami NR. 2021. Diversity of fungi associated with rhizosphere of healthy and diseased garlic crop. Biodiversitas 22: 1433-1440. Garlic (Allium sativum L.) is an especially important horticultural crop in Indonesia because it is used as a cooking spice and for medicines and cosmetic ingredients. It is mostly grown in irrigated highlands; however Indonesian production is only supplying at most 17.5% of domestic demand. West Nusa Tenggara (WNT) Province is one of the largest garlic-producing regions in Indonesia. Currently, garlic cultivation in WNT Province is facing a problem of root rot disease. Therefore, it is necessary to investigate the fungi associated with garlic root rot disease. Research was conducted in June – September 2018 and samples were collected from Sembalun highlands (1200 m above sea level). Seventeen fungal isolates were collected from the rhizosphere of the garlic crops and fourteen of them were identified. The fungi isolated from the rhizosphere of healthy garlic crops were from the genus Aspergillus, Penicillium, and Paecilomyces. While the fungi isolated from the rhizosphere of diseased garlic crops were from the genus Aspergillus, Penicillium, Paecilomyces, Fusarium, and Phoma. The genus Fusarium and Phoma are well known as plant pathogens.
“…Seven isolates (SL1, SL2, SL3, SL4, SL5, SL6, and SL7) of the endophytic fungi were obtained from Sembukan (Paederia foetida L.) leaves with di erent morphological colony characteristics. The diversity of endophytic fungi diversity in a plant occured due to the adaptation mechanism of the fungi to speci c microecology and physiological conditions of each host plant (Sopialena et al, 2018).…”
Section: Isolation Of the Endophytic Fungimentioning
The antibiotic resistance of phatogenic bacteria has become a serious health problem and has encouraged the search for novel and effective antimicrobial metabolites. Meanwhile, endophytic fungi have great potential as a natural source for antimicrobial agents. The endophytic fungi that live in plant tissue produces secondary metabolites which potentially act as an antibacterial compound. The isolation of fungi for antibacterial sources reduces the large amount of plant as a source of antibacterial agents. Hence, this study aims to obtain endophytic fungi isolates from Paederia foetida L. that are capable of producing secondary metabolites as antibacterial, carry out in vitro tests to verify the antibacterial properties of secondary metabolites of the Paederia foetida L. endophytic fungi, and identify the potential of Paederia foetida L. endophytic fungi in producing antibacterial compounds. The antibacterial activity was tested against Escherichia coli ATCC8739 and Staphylococcus aureus ATCC6538 while seven isolates of endophytic fungi that potentially produced antibacterial were obtained from Sembukan (P. foetida L.). The results showed that antibacterial activities of SL1, SL4 and SL6 secondary metabolites against S. aureus ATCC6538 and E. coli ATCC8739 were moderate to strong activities. Furthermore, the Minimum Inhibition Concentration (MIC) of secondary metabolites extract of SL1 against S. aureus ATCC6538 value was 250 ????g/mL while the values of MIC extract of SL4 against S. aureus ATCC6538 and E. coli ATCC8739 were 125 ????g/mL and 250 ????g/mL respectively and MIC extract of SL6 against E. coli ATCC8739 value was 125 ????g/mL. The secondary metabolites extract of SL1 isolate were alkaloid and tannin, SL4 were phenolic and alkaloid while SL6 isolate were alkaloid and terpenoid. Hence, endophytic fungi SL1 isolate was identified as Fusarium sp., SL4 as Dematophora sp., and SL6 isolate as Acremonium sp.
“…The endophytic fungi were isolated from the branch, stems, and roots of paddy. They were isolated using the method suggested by Sopialena et al [12] and Tumangger et al [7] with slight modification on plant surface sterilization [13]. The samples cut into ±1 cm and washed with running water.…”
Section: A Isolationand Identification Of Endophytic Fungimentioning
confidence: 99%
“…A total of 10paddy seed of were planted in a petri dish and incubated for 1 week. Observations were performed on the 7 days against germination healthy and show grains of rice's symptoms of necrosis [7,12].…”
Section: B Evaluation Of Endophytic Fungi Capability For Seedlingmentioning
Fusariumspis one of pathogenic fungi that cause wilt disease in paddy. However, they can be control by endophytic fungi which isolated within paddy plant itself. This study aims to isolate and evaluate the type of endophytic fungi from rice plant that have the potential to inhibit the growth of pathogenic fungus particular Fusarium sp. The endophytic fungi were purified and identified on the basis of their morphological and structural characters. Pathogenic evaluation was performed in vitro and in vivo for anti-fungi and germination consisting of 5 treatments and 5 replications. The obtained data was analysed using One Way ANNOVA. The results found the two endophytic fungi associated in paddy namely Nigrospora sp and Trichoderma sp. Between those, Tricoderma sp exhibited the higher germination (80%) and inhibitory power (45.7%) compared to another. Hence, this study recommended Tricoderma sp for further anti-fungi research against Fusarium sp.
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