Abstract. Sopialena, Rosfiansyah, Sila S. 2017. The benefit of top soil and fertilizer mixture to improve the ex-coal mining land. Nusantara Bioscience 9: 36-43. The mining activities have led severe impacts on some environmental issues including a decrease in soil fertility, which affects the ability of soil to supply soil nutrients to growing plants, a destruction in soil structure, and the loss of microorganisms, which are important for reforming process to treat organic materials. In this regards, reclamation of mined degraded land has been continually used to improve soil structure and its microorganisms. In practice, for large scale reclamation, fertilizer use can primarily enhance soil qualities in improving plant's growth and yield. It is suggested that healthy soil should contain millions of microorganisms at the aggregation which leads to the improvement of soil nutrients and its structure, as the medium for plant growth. For this purpose, therefore, this study aimed to determine the impact of organic fertilizer (Ostindo) and top soil mixture on microbial diversity, soil fertility and the growth of sengon (Paraserianthes falcataria L. Nielsen). This study made use of a randomized block design, using four treatments and five replications including P0: without top soil and fertilizer, P1: with top soil but without fertilizer, P2: without top soil but with fertilizer, P3: mixture of top soil and fertilizer. The results showed that a mixture of top soil and fertilizer has successfully increased in the number of fungal genus, starting from two genus (Phytium and Penicillium) turning into five genus (Phytium, Fusarium, Penicillium, Aspergillus, and Rhizoctonia), as well as in the number of nematode genus, from two (Dorylaimus and Rhabditis) turning into five genus (Dorylaimus, Steinernema, Dorylaimus, Hoplolaimus, and Mononchus). This study indicated that the mixture has significantly improved soil health in ex-coal mining land, which was viable through the pH level (almost neutral), the significant increase of C, N and P 2 O 5 , and also the growth of Sengon. In conclusion, this study succeeds in demonstrating that the mixture of top soil and fertilizer has enhanced the microbial diversity, soil fertility, and sengon growth.
Sopialena, Suyadi, Sahil M, Nurdiana J. 2018. The diversity of endophytic fungi associated with Piper nigrum in the tropical areas: a recent study from Kutai Kartanegara, Indonesia. Biodiversitas 19: 2028-2034. This research aimed to identify the diversity of endophytes fungal in the root and leaves of Piper nigrum Linn. This research contributes to the knowledge gaps by focusing the discussion on the Endophytic fungal communities of Piper nigrum Linn in tropical areas at Kutai Kartanegara, Indonesia. Two certain plots within the study area were selected based on the different characteristics of topographic contours. The locations, named Plot A was characterized by ramps area, while plot B was sloping or hilly area. A total of 55 isolates were obtained from the root and the leaves of healthy plants, 35 isolates were from Plot A and the other 20 isolates were from plot B. Based on Shannon index (H’), both of the plots corresponded to the same genus; namely Aspergillus sp., Fusarium sp., Nigrospora sp., and Trichoderma sp, and categorized as medium diversity. In this regards, this study confirms that the genus Aspergillus sp. and Fusarium sp. represent a large part of the diversity of Endophytes fungi. As the study indicates that plot B has higher diversity of endophytic fungi compared to plot A, it shows that the hilly area turns out to be a better location for Piper nigrum Linn.
Penelitian dilakukan untuk menentukan: cendawan endofit yang terdapat pada tanaman padi (Oryza sativa); persentase daya hambat cendawan endofit terhadap cendawan Pyricularia oryzae Cav.; dan mekanisme antagonis antara masing-masing cendawan endofit terhadap cendawan Pyricularia oryzae Cav.. Penelitian dilakukan di laboratorium terpadu Fakultas Pertanian Universitas Mulawarman, dan sampel tanaman yang terinfeksi serta tanaman sehat dikumpulkan dari Kelurahan Sungai Kapih, Kecamatan Sambutan, Kota Samarinda. Hasil penelitian disusun dalam Rancangan Acak Lengkap (RAL), yang terdiri dari empat sampel dan setiap sampel diulang sebanyak sepuluh kali. Hasil penelitian menunjukkan bahwa cendawan endofit yang terisolasi dari tanaman padi (Oryza sativa) adalah Trichoderma sp., Rhizopus sp., Gliocladium sp. dan Penicillium sp. Kompetisiterbukti secara in vitro sebagai mekanisme antagonis Rhizopus sp., sedangkan antibiosis terbukti secara in vitro sebagai mekanisme Penicillium sp.. Trichoderma sp dan Gliocladium sp terbukti memiliki dua mekanisme antagonis yaitu kompetisi dan parasitisme. Kemampuan cendawan endofit sebagai agen biologis untuk bersaing dengan cendawan P. oryzae itu menghasikan perbedaan daya antagonisme, tingkat tertinggi penghambatan terjadi padaP.oryzae vs Gliocladium sp. (78,96%).
Abstract. Sumartini. 2017. Short Communication: Biocontrol activity of Phyllosphere fungi on mungbean leaves againstCercospora canescens. . Examination of biocontrol activity of phyllosphere fungi on mungbean leaves against Cercospora canescens, a causal agent of mungbean leaf spot disease, was conducted during February-July 2016. Samples of symptomatic mungbean leaves were collected from several production areas in East and Central Java. Symptomatic leaves with leaf spot were collected randomly by detaching the symptomatic leaves and kept in a plastic bag for laboratory studies. Fungi associated with leaf spot were isolated using surface sterilized technique on PDA medium. In vitro and in vivo antagonism assay was carried out in the laboratory and greenhouse, respectively. Fungi associated with leaf spot on mungbeans such as Fusarium spp., Curvularia spp., Aspergillus flavus, and Nigrospora sp. were tested against C. canescens in the antagonism assay. Highest inhibition activity against C. canescens was found on Fusarium sp. 2 (KH-KJP-1B) (in vitro = 61%, in vivo = 19.63%) and Curvularia sp.1 (KH-JBG-B) (in vitro = 66%, in vivo = 16.46%).
Endemic leaf rust disease always occurs in almost all maize plantations in Indonesia. Furthermore, the development of this disease differs concurrently and is greatly influenced by the ecological conditions of maize cultivation. Therefore, this study fills the epidemiological gap of diseases that has not been conducted against the epidemiology of maize rust. This identifies the causes of leaf rust that attacked the maize plants in two locations, namely Bayur and Muang Dalam, Lempake, Samarinda, Indonesia. This study also analyzed the relationship or model between ecological factors of temperature, humidity, and soil fertility on the intensity of leaf rust and the infection rate of maize leaf rust. Measurement of disease intensity, the rate at which it developed, soil fertility and temperature and humidity of the area are conducted in this study. Meanwhile, soil fertility also influenced disease progression and the nutrient-poor soils in two sites cause leaf rust disease to develop well. The identification results showed that the cause of maize leaf rust was <em>Puccinia sorghi</em> Schw. Therefore, the temperature accompanied by the increased humidity is directly proportional to the development of the leaf rust.
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