The Difference of Effectivity Between Mangosteen Peel Extract and Metronidazole on Fibroblast Proliferation

Atiqah, Adinda Nur; Poetri, Adisty Restu; Niam, Moh Husnun

doi:10.30659/odj.8.1.80-85

Cited by 3 publications

(4 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This shows that administration of metronidazole gel can increase the number of fibroblasts, metronidazole gel eliminates P. gingivalis by interfering with DNA synthesis and bacterial cell nuclei to prevent colonization or further infection. Thus, inflammation can occur in a short time and be followed by tissue regeneration, one of which is the process of fibroblast proliferation (Atiqah et al, 2021;Cialdai et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

The Effect of Cocoa Pod Husk (Theobroma cacao L.) Extract on Increasing Gingival Fibroblast and Collagen Density in Wistar Rats Induced by Porphyromonas gingivalis

Rahayu,

et al. 2024

IJMRA

View full text Add to dashboard Cite

Periodontal disease is an inflammatory disease of the oral cavity which characterized by inflammation of the gingiva. Theobroma cacao L. is a herbal plant that has antibacterial and anti-inflammatory effects, which is starting to be widely researched as an alternative therapy for inflammation in the oral cavity. The aim of this study was to examined the effect of the ethanol extract gel of cocoa pod husk (Theobroma cacao L.) on increasing the number of fibroblasts and the collagen fiber density in the gingiva of rats that have been induced by Phorphyromonas gingivalis. The sample used 24 male Wistar rats divided into 3 groups. Each group was induced by porphyromonas gingivalis every 3 days for 14 days then anointed with placebo gel (negative control group), metronidazole gel (positive control group), and cocoa pod ethanol extract gel (CPH gel) for 7 and 14 days. Gingival tissue was taken after euthanasia, after which histological preparations were made, and fibroblasts were observed under a microscope and analyzed using quantitative pathology 0.3.2 software, while for collagen density analized using Adobe Photoshop CS6 software. The results showed there was a significant difference of the number of fibroblasts between each group (p<0.05). however there was no significant difference between positive control and cocoa pod husk extract at day 14 (p > 0.05).The treatment group on day 7 and 14 was higher than the other groups. It was conclude that the ethanol extract of cacao pod husk (Theobroma cacao L.) potential to increase the number of fibroblasts and collagen fibers density in gingival tissue of periodontitis rats.

show abstract

Section: Discussionmentioning

confidence: 99%

The Effect of Cocoa Pod Husk (Theobroma cacao L.) Extract on Increasing Gingival Fibroblast and Collagen Density in Wistar Rats Induced by Porphyromonas gingivalis

Rahayu,

et al. 2024

IJMRA

View full text Add to dashboard Cite

show abstract

“…Thus, inflammation can occur in a short time and is followed by tissue regeneration, such as the process of fibroblast proliferation. 28 …”

Section: Discussionmentioning

confidence: 99%

“… 33 By promoting collagen synthesis, reducing macrophages and tissue edema, and boosting fibroblast production, flavonoids promote wound healing. 28 During the proliferative phase, fibroblast cells gradually form on the surface of the wound and generate fresh collagen fibers. A new collagen fiber will be formed to replace any damaged fibers of collagen that have an uneven shape.…”

Section: Discussionmentioning

confidence: 99%

Increasing fibroblasts and gingival collagen density in periodontitis rats by using cassava leaf extract

Shita,

Dharmayanti,

Meilawaty

et al. 2023

Journal of Taibah University Medical Sciences

View full text Add to dashboard Cite

“…The role of metronidazole can inhibit bacterial DNA replication and cause bacterial death. Bacterial death can prevent excess bacterial colonization so that a more severe infection does not occur so that the inflammatory phase occurs in a shorter time [25].…”

Section: Groupsmentioning

confidence: 99%

Effects of Cocoa Pod Husk Extract (Theobroma Cacao L.) on Alveolar Bone in Experimental Periodontitis Rats

Rahayu

Setiawatie²,

Rahayu³

et al. 2023

Trends Sci

View full text Add to dashboard Cite

Periodontitis is a destructive inflammatory disease of the periodontal tissues with a high prevalence in the world. Phorphyromonas gingivalis as one of the main bacteria causing periodontitis produces virulence factors that trigger immune cells to produce proinflammatory cytokines that can reduce the number of osteoblasts, thereby triggering alveolar bone resorption. Osteoblast cell differentiation is mediated by a number of growth factors such as cytokines and bone morphogenic proteins (BMPs). SRP mechanical therapy is sometimes not optimal and Metronidazole as an effective antibiotic against periodontitis has been reported to have several adverse effects. Utilization of cocoa pod waste (Theobroma cacao L) with its polyphenol content has antibacterial, antioxidant and anti-inflammatory effects. This study aims to determine the effect of ethanol extract gel from cocoa pod husk extract (Theobroma cacao L.) on the number of osteoblasts and osteoclasts, as well as the expression of BMP-2 in the alveolar bone of periodontitis-model rats. This laboratory experimental study used 24 rats which were divided into negative control group (CMC-Na gel), positive control group (metronidazole gel), and treatment group (100 mg/mL cocoa pod husk extract gel). 24 Wistar rats were divided into 3 groups which were observed on days 7 and 14. The number of osteoblasts and osteoclasts of alveolar bone were observed by hematoxillin eosin staining and BMP-2 expression by immunohistochemistry staining. One-way ANOVA test showed that there was a significant difference (p < 0.05) between groups. The LSD test average of BMP-2 expression in the treatment group on the day 7 and day 14 was higher than the negative control group (p < 0.05). The conclusion of the study was that giving 100 mg/mL of cacao pod husk extract gel (Theobroma cacao L.) increased the number of osteoblasts and increased the expression of BMP-2 in the alveolar bone of periodontitis rats. HIGHLIGHTS Cocoa pod husk extract gel (Theobroma cacao) 100 mg/mL increased the ratio of osteoblasts to osteoclasts and increased BMP-2 expression in the alveolar bone of periodontitis rats The histological staining with IHC was analyzed using Quantitative Pathology 0.3.2 software The cocoa pod husk (Theobroma Cacao L.) extract gel effectively triggers and increased BMP-2 which stimulates bone formation in periodontitis rat models GRAPHICAL ABSTRACT

show abstract

The Difference of Effectivity Between Mangosteen Peel Extract and Metronidazole on Fibroblast Proliferation

Cited by 3 publications

References 2 publications

The Effect of Cocoa Pod Husk (Theobroma cacao L.) Extract on Increasing Gingival Fibroblast and Collagen Density in Wistar Rats Induced by Porphyromonas gingivalis

The Effect of Cocoa Pod Husk (Theobroma cacao L.) Extract on Increasing Gingival Fibroblast and Collagen Density in Wistar Rats Induced by Porphyromonas gingivalis

Increasing fibroblasts and gingival collagen density in periodontitis rats by using cassava leaf extract

Effects of Cocoa Pod Husk Extract (Theobroma Cacao L.) on Alveolar Bone in Experimental Periodontitis Rats

Contact Info

Product

Resources

About