The Comparison of Streptococcus agalactiae Isolated from Fish and Bovine using Multilocus Sequence Typing

Abstract

Multilocus sequence typing (MLST) has greater utility for determining the recent ancestral lineage and the relatedness of individual strains. Group B streptococci (GBS) is one of the major causes of subclinical mastitis of dairy cattle in several countries. GBS also sporadically causes epizootic infections in fish. The aim of this study was to compare the evolutionary lineage of fish and bovine isolates in relation to the S. agalactiae global population as a whole by comparing the MLST profiles. Twenty S. agalactiae isolates were obtained from dairy cattle and fish. PCR products were amplified with seven different oligonucleotide primer pairs designed from the NEM316 GBS genome sequence. Clone complexes demonstrated that bovine and fish isolates were separate populations. These findings lead us to conclude that fish S. agalactiae is not a zoonotic agent for bovine. MLST could help clarify the emergence of pathogenic clones and to decide whether the host acts as a reservoir for another pathogenic lineage.

Molecular serotyping, virulence gene profiling and pathogenicity of Streptococcus agalactiae isolated from tilapia farms in Thailand by multiplex PCR

“…Both techniques are widely used to discriminate S. agalactiae , and they also provide valuable information such as the genetic differentiation between fish, human and bovine isolates (Lusiastuti et al . ; Chu et al . ).…”

Impacts of streptococcal infection on Nile tilapia Oreochromis niloticus in Egyptian farms with special reference to diagnosis and prevention

“…The loop-mediated isothermal amplification method (LAMP) is employed to quickly discover the fbsB gene in S. agalactiae (Wang et al, 2012). Multi Locus Sequence Typing was also utilized for identification purposes (Lusiastuti et al, 2013;. Identification of streptococcus species is also done by the MALDI-TOF-MS technique (Kim et al, 2017;Souter et al, 2021).…”

Aeromonas hydrophila AHL 0905-2 and Streptococcus agalactiae N14G as Combined Vaccine Candidates for Nile Tilapia

“…The two sets of universal primer targeting prokaryotic 16S rDNA used in this study (Table 2) included F/R (Nam and Joh et al 2007) for isolates of Aeromonas spp. and S. agalactiae (Agal I F1 and Agal II R) (Yildirim 2002;Lusiastuti et al 2013). The PCR mixtures contained 10 pMol of each primer pair, 12.5 µl master mix (GoTaq®Green, Promega, USA), and 2 µl of DNA template in a final volume of 25 µl.…”