Molecular serotyping, virulence gene profiling and pathogenicity of
            <i>Streptococcus agalactiae</i>
            isolated from tilapia farms in Thailand by multiplex
            <scp>PCR</scp>

Kannika, Korntip; Pisuttharachai, Duangjai; Srisapoome, Prapansak; Wongtavatchai, Janenuj; Kondo, Hidehiro; Hirono, Ikuo; Unajak, Sasimanas; Areechon, Nontawith

doi:10.1111/jam.13447

Cited by 72 publications

(71 citation statements)

References 48 publications

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“…The experimental fish were anesthetized with clove oil to minimize stress during vaccination and challenge testing. S. agalactiae serotypes Ia and III were cultured as described previously 7 . S. agalactiae serotype III was used for polyclonal antibody (pAb) production, which was kindly provided by Prof. Ikuo Hirono, TUMSAT, Japan.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Novel Chimeric Multiepitope Vaccine for Streptococcosis Disease in Nile Tilapia (Oreochromis niloticus Linn.)

Pumchan

Krobthong

Roytrakul

et al. 2020

Sci Rep

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Streptococcus agalactiae is a causative agent of streptococcosis disease in various fish species, includingnile tilapia (Oreochromis niloticus Linn.). Vaccination is an effective disease prevention and control method, but limitations remain for protecting against catastrophic mortality of fish infected with different strains of streptococci. Immunoproteomics analysis of S. agalactiae was used to identify antigenic proteins and construct a chimeric multiepitope vaccine. Epitopes from five antigenic proteins were shuffled in five helices of a flavodoxin backbone, and in silico analysis predicted a suitable RnA and protein structure for protein expression. 45F2 and 42E2 were identified as the best candidates for a chimeric multiepitope vaccine. Recombinant plasmids were constructed to produce a recombinant protein vaccine and DNA vaccine system. Overexpressed proteins were determined to be 30 kDa and 25 kDa in the E. coli and TK1 systems, respectively. The efficacy of the chimeric multiepitope construct as a recombinant protein vaccine and DnA vaccine was evaluated in nile tilapia, followed by S. agalactiae challenge at 1 × 10 7 cfU/mL. Relative percentage survival (RpS) and cumulative mortality were recorded at approximately 57-76% and 17-30%, respectively. These chimeric multiepitope vaccines should be applied in streptococcosis disease control and developed into a multivalent vaccine to control multiple diseases.

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Section: Methodsmentioning

confidence: 99%

“…Mortality and clinical signs of infected tilapia were recorded daily for 3 weeks. The brain, head kidney, and liver were collected from moribund fish for bacterial isolation and identification 7 . Cumulative mortality and relative percentage survival (RPS) were calculated 60 .…”

Section: Vaccine Efficacy Analysismentioning

confidence: 99%

Novel Chimeric Multiepitope Vaccine for Streptococcosis Disease in Nile Tilapia (Oreochromis niloticus Linn.)

Pumchan

Krobthong

Roytrakul

et al. 2020

Sci Rep

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“…Serotype Ib strains isolated in Central and South America are ST-260 and ST-552 in CC552 (27, 29) and strains isolated in Australia, Israel, Belgium, China, Ghana, USA and Southeast Asia belong to ST-261 (5, 6, 9, 13, 29–31). Serotype III is commonly causative of disease in humans, but has also been isolated from fish in Thailand, China and recently in Brazil (26, 32–34).…”

Section: Introductionmentioning

confidence: 99%

Microevolution of aquaticStreptococcus agalactiaeST-261 from Australia indicates dissemination via imported tilapia and ongoing adaptation to marine hosts or environment

Kawasaki

Delamare-Deboutteville

Bowater³

et al. 2018

Preprint

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Streptococcus agalactiae (GBS) causes disease in a wide range of animals. The serotype 1b lineage is highly adapted to aquatic hosts, exhibiting substantial genome reduction compared with terrestrial con-specifics. Here we sequence genomes from 40 GBS isolates including 25 from wild fish and captive stingrays in Australia, six local veterinary or human clinical isolates, and nine isolates from farmed tilapia in Honduras and compare with 42 genomes from public databases. Phylogenetic analysis based on non-recombinant core genome SNPs indicated that aquatic serotype Ib isolates from Queensland were distantly related to local veterinary and human clinical isolates. In contrast, Australian aquatic isolates are most closely related to a tilapia isolate from Israel, differing by only 63 core-genome SNPs. A consensus minimum spanning tree based on core genome SNPs indicates dissemination of ST-261 from an ancestral tilapia strain, which is congruent with several introductions of tilapia into Australia from Israel during the 1970s and 1980s. Pan-genome analysis identified 1,440 genes as core with the majority being dispensable or strain-specific with non-protein-coding intergenic regions (IGRs) divided amongst core and strain-specific genes. Aquatic serotype Ib strains have lost many virulence factors during adaptation, but six adhesins were well conserved across the aquatic isolates and might be critical for virulence in fish and targets for vaccine development. The close relationship amongst recent ST-261 isolates from Ghana, USA and China with the Israeli tilapia isolate from 1988 implicates the global trade in tilapia seed for aquaculture in the widespread dissemination of serotype Ib fish-adapted GBS.ImportanceStreptococcus agalactiae (GBS) is a significant pathogen of humans and animals. Some lineages have become adapted to particular hosts and serotype Ib is highly specialized to fish. Here we show that this lineage is likely to have been distributed widely by the global trade in tilapia for aquaculture, with probable introduction into Australia in the 1970s and subsequent dissemination in wild fish populations. We report variability in the polysaccharide capsule amongst this lineage, but identify a cohort common surface proteins that may be a focus of future vaccine development to reduce the biosecurity risk in international fish trade.

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“…GBS was initially described in 1887 as an animal pathogen responsible for mastitis in ruminants and although largely eradicated in some countries with a highly developed dairy industry, remains a major cause of bovine mastitis in many countries 1 . In 1966, GBS infection of fish was reported in the USA 2 with subsequent outbreaks described in fish farms in Asia and the Americas 3,4 . GBS infection is often associated with high levels of mortality in fish and with the worldwide growth of aquaculture has significant economic impact 5 .…”

Section: Introductionmentioning

confidence: 99%

Galleria mellonellaas an infection model for the multi-host pathogenStreptococcus agalactiaereflects hypervirulence of ST283

Kranjangwong

Crumlish

et al. 2018

Preprint

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Streptococcus agalactiae, or group B streptococcus (GBS), infects diverse hosts including humans, economically important livestock and fishes. In the context of human health, GBS is a major cause of neonatal infections and an emerging cause of invasive disease in adults. Here we show that GBS is able to establish a systemic infection in G. mellonella larvae that is associated with extensive bacterial replication and dose dependent larval survival. This infection model is suitable for use with GBS isolates from both homeothermic and poikilothermic hosts and a hypervirulent sequence type (ST) associated with invasive human disease, ST283, shows increased virulence in this model, indicating it may be useful in studying GBS virulence determinants. In addition, we demonstrate that larval survival can be afforded by antibiotic treatment and so the model may also be useful in the development of novel anti-GBS strategies.The use of G. mellonella in GBS research has the potential to provide a low cost infection model that could reduce the number of vertebrates used in the study of GBS infection.

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Molecular serotyping, virulence gene profiling and pathogenicity of Streptococcus agalactiae isolated from tilapia farms in Thailand by multiplex PCR

Cited by 72 publications

References 48 publications

Novel Chimeric Multiepitope Vaccine for Streptococcosis Disease in Nile Tilapia (Oreochromis niloticus Linn.)

Novel Chimeric Multiepitope Vaccine for Streptococcosis Disease in Nile Tilapia (Oreochromis niloticus Linn.)

Microevolution of aquaticStreptococcus agalactiaeST-261 from Australia indicates dissemination via imported tilapia and ongoing adaptation to marine hosts or environment

Galleria mellonellaas an infection model for the multi-host pathogenStreptococcus agalactiaereflects hypervirulence of ST283

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