The Comparison ofStaphylococcus aureusIsolated From Blood and Wound Specimens for Genes Encoding Polysaccharide Intercellular Adhesion (PIA)

Abstract

Background:The polysaccharide intercellular adhesion (PIA) produced by Staphylococcus aureus is effective in the protection of isolates from outer harsh conditions and progress of infection. Objectives: The aim of this study was to compare the icaABCD genes encoding polysaccharide intercellular adhesion (PIA) between blood and wound isolates of Staphylococcus aureus (S. aureus) in Tehran. Patients and Methods: Forty-eight clinical isolates (including 30 blood and 18 skin wounds) were collected from patients and were identified. Next, mecA gene, SCCmec types and icaABCD genes were detected among blood and wound isolates of S. aureus by PCR assay and specific primers. Results: Nine (19%) out of 12 methicillin resistant S. aureus (MRSA) isolates harbored SCCmec type III and three (6.2%) isolates harbored SCCmec type V. Prevalence of icaA, icaB, icaC and icaD in blood isolates was twenty-one (70%), fourteen (48%), nineteen (64%) and eighteen (60%), respectively; while the prevalence in wound isolates was as nine (50%), seven (39%), six (34%) and twelve (67%), respectively. Conclusions: These findings showed no significant difference regarding the presence of icaADBC genes between blood and wound isolates.

StaphylococcalCassette Chromosomemec(SCCmec) Typing of Methicillin-ResistantStaphylococcus aureusStrains Isolated from Community- and Hospital-Acquired Infections

“…While SCCmec type III (35.18%) showed the highest prevalence in the present study, frequency of SCCmec type V (5.55%) was low. These results were consistent with previous studies performed in Iran and other countries (24,27,30,31).…”