StaphylococcalCassette Chromosomemec(SCCmec) Typing of Methicillin-ResistantStaphylococcus aureusStrains Isolated from Community- and Hospital-Acquired Infections

Ghanbari, Fahimeh; Saberianpour, Shirin; Zarkesh-Esfahani, Fatemeh-sadat; Ghanbari, Nafiseh; Taraghian, Azadeh; Khademi, Farzad

doi:10.5812/ajcmi.42244

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…This evidence leads us to conclude that HA-MRSA strains collected in present survey may be linked to the ST239 clone. Similar to other studies [ 15 , [19] , [20] , [21] ], in the present study, cassette types I and II represented a minority, with frequencies of 7.5% and 10.1% respectively. Conversely, Chongtrakool et al.…”

Section: Discussionsupporting

confidence: 91%

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First report of methicillin-resistant Staphylococcus aureus carrying the mecC gene in human samples from Iran: prevalence and molecular characteristics

Goudarzi

Navidinia

Dadashi

et al. 2021

New Microbes and New Infections

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Section: Discussionsupporting

confidence: 91%

“…(28%) [ 19 ] and Ghanbari et al. (35.2%) [ 20 ] in Iran. According to our findings, SCC mec type III is responsible for most of the SCC mec types in HA-MRSA infections.…”

Section: Discussionmentioning

confidence: 99%

First report of methicillin-resistant Staphylococcus aureus carrying the mecC gene in human samples from Iran: prevalence and molecular characteristics

Goudarzi

Navidinia

Dadashi

et al. 2021

New Microbes and New Infections

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“…We previously found VRSA in the trachea sample of a dead patient in Mashhad, northeastern Iran (12). The resistance to oxacillin in wound isolated S. aureus strians was reported in many reports worldwide (21-23), but resistance to vancomycin in these isolates is not prevalent (24). In recent years, some researchers reported mecA -MRSA isolates.…”

Section: Discussionmentioning

confidence: 96%

Isolation and Genetic Characterization of Vancomycin-resistant and mecC+ Methicillin-resistant Staphylococcus aureus Strains in Clinical Samples of Bojnurd, Northeastern Iran

Moghaddam

Azimian

Sepahy

et al. 2021

Jundishapur J Microbiol

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Background: The emergence of antibiotic-resistant Staphylococcus aureus strains is one of the major concerns about the various staphylococcal infections. Vancomycin is one the most important effective antibiotics on staphylococcal lethal infections. To date, vancomycin-resistant strains are increasingly isolated in different parts of the world, and it is alerting. Objectives: The current study was designed to evaluate the prevalence, and antibiotic susceptibility pattern of methicillin-resistant S. aureus (MRSA) and vancomycin-resistant S. aureus (VRSA) isolates in the main tertiary hospital of Bojnurd, Iran. Methods: S. aureus isolates were collected from different clinical samples in Imam Reza Hospital of Bojnurd. After identification of isolates through using conventional methods, they were evaluated by agar screening, disk diffusion, and minimum inhibitory concentration (MIC) methods to determine resistance to vancomycin and methicillin. We also performed polymerase chain reaction (PCR) for the detection of mecA, mecC, vanA, and vanB genes. After confirmation of vancomycin resistance, genetic analysis was performed using SCCmec, agr, and spa typing, and multilocus sequence typing (MLST) methods on VRSA isolates. Results: We found four vancomycin-resistant isolates (1.29%). Also, 75% of isolates were resistant to cefoxitin. Using the PCR method, mecA was found in 73%, mecC in 0.64%, and vanA in 1.29% of isolates. Interestingly, we found two mecC positive isolates in MRSA isolates. The alpha-hemolysin (81.81%) and enterotoxin C (27%) had the highest and lowest toxins percentage, respectively. Among mecA positive isolates, SCCmecIV (37%), SCCmecIII (31.27%), SCCmecI (14%), SCCmecII (11%), and SCCmecV (5.7%) were the most prevalent SCCmec types, respectively. It should be noted that the two mecC positive isolates belonged to SCCmecXI. AgrI (76.29%) was the highest agr type. We recognized t037 as the dominant spa type, and ST239, ST6, ST97, and ST8 were found in VRSA isolates. Conclusions: In our study, the frequency of mecA genes in MRSA isolates was very high. It seems that the resistant isolates belonged to endemic clones of Iran.

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“…The goal of this study was to determine the diversity of common clones, prevalence of virulence genes and antibiotic susceptibility patterns of MRSA strains in four University Teaching Hospitals in the center of Iran. It has been documented that MRSA clones are changing, and CA-MRSA are expanding into healthcare settings [ 23 ]. According to different previous studies, SCC mec types I-III and IV-V are commonly responsible for HA-MRSA and CA-MRSA infections, respectively [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of methicillin-resistant Staphylococcus aureus isolates from four teaching hospitals in Iran: the emergence of novel MRSA clones

Firoozeh

Omidi

Saffari

et al. 2020

Antimicrob Resist Infect Control

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Background: The global spread of methicillin-resistant Staphylococcus aureus (MRSA) infections necessitates the use of validated methods for the identification and typing of this bacterium. This study aimed to determine the distribution of main molecular types of MRSA strain circulating among hospitalized patients in teaching hospitals in Isfahan and Kashan. Methods: A total of 146 Staphylococcus aureus strains were isolated from patients in four teaching hospitals in Isfahan and Kashan during June 2017 to September 2018. The antimicrobial resistance patterns of Staphylococcus aureus strains were performed by disc diffusion method. The MRSA strains were identified phenotypically and confirmed by PCR assay. The prevalence of microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) genes among MRSA strains was evaluated by multiplex PCR. The genotypes of MRSA strains were determined by multilocus sequence typing and SCCmec typing. Results: Of 146 Staphylococcus aureus isolates, 24 (16.4%) isolates were identified as MRSA strains. According to antimicrobial susceptibility testing the highest resistance rates were seen for tetracycline, erythromycin, ciprofloxacin and gentamicin. All of Staphylococcus aureus isolates were susceptible to vancomycin whereas 3 (2.1%) isolates were resistant to linezolid. Three different SCCmec types were obtained among MRSA strains including 16 (66.7%) SCCmec type V, 3 (12.5%) SCCmec type III and 5 (20.8%) SCCmec type II. Of 24 MRSA isolates 20 (83.3%) carried MSCRAMMs genes including eno (70.8%), fib (54.1%), cna (25.0%), fnbB (16.6%), ebps 5 (20.8%), and the fnbA, bbp and clfA genes were not detected in any MRSA isolate. MLST analysis revealed 11 sequence types among MRSA isolates as follows:

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StaphylococcalCassette Chromosomemec(SCCmec) Typing of Methicillin-ResistantStaphylococcus aureusStrains Isolated from Community- and Hospital-Acquired Infections

Abstract: Background: Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for the growing number of hospital-and community-acquired infections.

Cited by 11 publications

References 25 publications

First report of methicillin-resistant Staphylococcus aureus carrying the mecC gene in human samples from Iran: prevalence and molecular characteristics

First report of methicillin-resistant Staphylococcus aureus carrying the mecC gene in human samples from Iran: prevalence and molecular characteristics

Isolation and Genetic Characterization of Vancomycin-resistant and mecC+ Methicillin-resistant Staphylococcus aureus Strains in Clinical Samples of Bojnurd, Northeastern Iran

Molecular analysis of methicillin-resistant Staphylococcus aureus isolates from four teaching hospitals in Iran: the emergence of novel MRSA clones

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