The anaesthetic effect of camphor (Cinnamomum camphora), clove (Syzygium aromaticum) and mint (Mentha arvensis) essential oils on clown anemonefish,Amphiprion ocellaris(Cuvier 1830)

Pedrazzani, Ana Silvia; Ostrensky, Antônio

doi:10.1111/are.12535

Cited by 33 publications

(29 citation statements)

References 23 publications

(37 reference statements)

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“…Initially, pilot tests were conducted following the method proposed by Pedrazzani and Ostrensky (2014) to determine the oil concentrations to be tested in this study. Then, the fish were submitted to five different concentrations of each EO (n=10/concentration) previously defined (clove: 40, 50, 60, 70 and 80 µL L ) .…”

Section: Methodsmentioning

confidence: 99%

“…Some essential oils (EO) derived from plants have been shown to be a viable alternative to reduce stress during fish capture and handling (Silva et al, 2013). Different oils have been used to anaesthetize fish, such as Eugenia caryophyllata (Weber et al, 2009;Pawar et al, 2011), Cinnamomum camphora, Mentha arvensis (Pedrazzani and Ostrensky, 2014), Melaleuca alternifolia (Hajek, 2011), Ocimum gratissimum (Benovit et al, 2012), Hesperozygis ringens and Ocotea acutifolia (Silva et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Basil, tea tree and clove essential oils as analgesics and anaesthetics in Amphiprion clarkii (Bennett, 1830)

et al. 2017

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In this study were evaluated the anaesthesia and analgesic effects of clove Eugenia caryophyllata, tea tree Melaleuca alternifolia and basil Ocimum basilicum essential oils (EO) during handling of yellowtail clownfish Amphiprion clarkii. Juveniles (3.70 ± 0.75 cm and 1.03 ± 0.50 g; mean ± standard deviation) were submitted to concentrations of tea tree oils (n=10/concentration), previously defined in pilot tests. Individually and only once, fish from each treatment were placed in a glass recipient containing 1 L of seawater at a temperature of 25 °C, salinity of 35 g L -1 and the specific concentration of diluted EO (stock solution). Control (only seawater) and blank (seawater and ethanol at the highest concentration used to dilute the oils) treatments were also conducted. After reaching the stage of surgical anaesthesia, fish were submitted to biometry and a sensibility test. After that, they were transferred to clean seawater for anaesthesia recovery. The times of induction needed to reach each anaesthesia stage and anaesthesia recovery were recorded. Animals were observed for 72 hours after the procedures. All the EO provoked anaesthesia and analgesic effects in A. clarkii, but basil oil is not recommended because it caused involuntary muscle contractions and mortality in 100% and 12% of fish, respectively. The lower concentrations that promote suitable induction and recovery times are 50 µl L -1 of clove oil and 500 µl L -1 of tea tree oil. However, due to its complementary high analgesic efficiency, clove oil is recommended as the ideal anaesthetic for A. clarkii. , 500 e 600 µl L -1 de melaleuca (n=10/concentração), previamente definidas em testes pilotos. Individualmente e somente uma vez, os peixes de cada tratamento foram colocados em recipiente de vidro contendo 1 L de água salgada, em temperatura de 25 °C, salinidade de 35 g L -1 e a concentração específica de OE diluída (solução estoque). Tratamentos controle (apenas água marinha) e branco (água marinha e a maior concentração de etanol utilizada para diluição dos óleos) também foram conduzidos. Após atingirem o estágio de anestesia cirúrgica, os peixes foram submetidos à biometria e teste de sensibilidade. Em seguida, foram transferidos para água marinha limpa. Os tempos necessários para atingir cada estágio anestésico e recuperação foram registrados. Os animais foram observados por 72 horas após os procedimentos. Todos os OE provocaram anestesia e analgesia em A. clarkii, porém o óleo de manjericão não é recomendado, pois causou contrações musculares involuntárias e mortalidade em 100% e 12% dos animais, respectivamente. As menores concentrações que promovem indução anestésica e recuperação em tempos adequados são 50 µl L -1 de óleo de cravo e 500 µl L -1 de óleo de melaleuca. Entretanto, devido à sua alta eficiência analgésica complementar, o óleo de cravo é recomendado como o anestésico ideal para A. clarkii. Keywords

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Basil, tea tree and clove essential oils as analgesics and anaesthetics in Amphiprion clarkii (Bennett, 1830)

et al. 2017

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“…The pilot experiments followed the method proposed by Pedrazzani and Ostrensky (2014). A fish was placed in a glass container with the respective anaesthetic at the desired concentration.…”

Section: Determination Of Effective Anaesthetic Concentrationsmentioning

confidence: 99%

Use of MS-222 (tricaine methanesulfonate) and propofol (2,6-diisopropylphenol) as anaesthetics for the tetraAstyanax altiparanae(Teleostei, Characidae)

2015

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The aim of this study was to evaluate the anaesthetic effect of MS-222 and propofol and determine their optimal concentrations for safe handling of the tetra Astyanax altiparanae in the laboratory. The fish were separated by length into three classes: I (1.5-5.0 cm), II (5.1-8.0 cm) and III (greater than 8.1 cm). Pilot tests were performed to evaluate the appropriate anaesthetic concentrations for inducing the five possible anaesthetic stages: I -sedation; II -light anaesthesia; IIIdeep anaesthesia; IV -surgical anaesthesia; and V -spinal collapse. After defining the maximum and minimum concentrations required to induce stage IV anaesthesia, the animals were exposed to five intermediate concentrations (n = 10 fish/concentration) of each anaesthetic for 15 min. The animals were then transferred to clean water to evaluate the time required for recovery. In addition, blood glucose levels were measured for class II and class III fish subjected to the previously defined ideal concentrations for each of the tested anaesthetics (n = 10 fish/treatment). Both evaluated substances are suitable to anaesthetize A. altiparanae. The optimal MS-222 concentration was 90 mg L À1 , and this result was similar for all three size classes. The optimal propofol concentrations for inducing surgical anaesthesia in the size classes I, II and III were 0.22, 0.23 and 0.27 respectively.

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“…) and mentha (Danner et al . ; Roohi & Imanpoor ; Pedrazzani & Neto ), among others (Benovit et al . ; Parodi et al .…”

Section: Future Trendsmentioning

confidence: 97%

Trends in aquaculture sciences: from now to use of nanotechnology for disease control

Luis

Campos

Oliveira

et al. 2017

Reviews in Aquaculture

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Aquaculture is increasingly important in global food production. Consequently, the control of diseases in aquaculture is essential, due to the potential environmental impacts, notably in terms of the states of health of freshwater bodies and the oceans. In this study, we review the possible uses of different management systems, highlighting essential oils and novel nanotechnological strategies, for the control of diseases in aquaculture.

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The anaesthetic effect of camphor (Cinnamomum camphora), clove (Syzygium aromaticum) and mint (Mentha arvensis) essential oils on clown anemonefish,Amphiprion ocellaris(Cuvier 1830)

Cited by 33 publications

References 23 publications

Basil, tea tree and clove essential oils as analgesics and anaesthetics in Amphiprion clarkii (Bennett, 1830)

Basil, tea tree and clove essential oils as analgesics and anaesthetics in Amphiprion clarkii (Bennett, 1830)

Use of MS-222 (tricaine methanesulfonate) and propofol (2,6-diisopropylphenol) as anaesthetics for the tetraAstyanax altiparanae(Teleostei, Characidae)

Trends in aquaculture sciences: from now to use of nanotechnology for disease control

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