Texas Sour Orange Juice Used in Scaffolds for Tissue Engineering

Abstract

Fine fibers of polyhydroxybutyrate (PHB), a biopolymer, were developed via a centrifugal spinning technique. The developed fibers have an average diameter of 1.8 µm. Texas sour orange juice (SOJ) was applied as a natural antibacterial agent and infiltrated within the fibrous membranes. The antibacterial activity against common Gram-positive and Gram-negative bacteria (Staphylococcus aureus and Escherichia coli, respectively) was evaluated as well as cell adhesion and viability. The PHB/SOJ scaffolds showed antibacterial activity of up to 152% and 71% against S. aureus and E. coli, respectively. The cell studies revealed a suitable environment for cell growth and cell attachment. The outcome of this study opens up new opportunities for fabrication of fibrous materials for biomedical applications having multifunctional properties while using natural agents.

Tunable CsPb(Br/Cl)3perovskite nanocrystals and further advancement in designing light emitting fiber membranes

“…Given that electrical fields are not needed, it broadens the choice of materials to be used since dielectric properties are not a factor to be considered. [40,41] Additionally, higher production rates and the ease of processing [42], have prompted attention to this technology to develop fiber membrane systems for a wide variety of applications for example antibacterial gauze, [43] tissue engineering, [44] air filtration, [45] optical sensors, [46] lithium ion battery, [47] photo luminescence, etc. [48] In this study we have synthesized blue and green photoluminescent CsPb(Br/Cl) 3 perovskite NCs and have used these to develop fine fiber membranes from CsPb(Br/Cl) 3 /polymer solutions by using the centrifugal spinning method.…”

Recent Advances in Centrifugal Spinning and Their Applications in Tissue Engineering

“…Reducing infection in wound healing and treatment, sour orange juice (SOJ) can be incorporated into polyhydroxy butyrate (PHB) fibers as an antibacterial agent with improved cell adhesion and proliferation. PHB fibers showed the effectiveness of trapping liquid agents into their system, providing a moist medium to promote healing, as illustrated in Figure 9 [86]. The PHB/SOJ scaffolds showed antibacterial activity of up to 152% and 71% after an antibacterial test conducted using the disk diffusion and dilution methods.…”

“…Development of such 3D scaffolds has been introduced for cell infiltration, migration, adhesion, and growth [30]. Cell adhesion is favored by fibers with smaller diameters when compared to large-sized fibers [33,86]. Recently, many investigations have been started to pay attention to making scaffolds with synthetic biopolymers or biodegradable polymer nanofibers.…”

Cell proliferative properties of Forcespinning®nopal composite nanofibers

“…Conversely, Forcespinning ® (FS), based on the use of centrifugal force has the ability to mass produce fibers from a vast array of polymers without any limitation concerning dielectric properties of the system to be spun. [28][29][30] Previously, we developed chitosan (CH) and pullulan (PL) nanofibers incorporating varying concentrations of O. cochenillifera "nopal" mucilage (N) extract with antibacterial properties. 31 Here, we explore the ability of N/ CH/PL composite nanofibers to support cell adhesion and proliferation compared to control CH/PL composite nanofibers lacking N extract.…”