Cell proliferative properties of Forcespinning^®nopal composite nanofibers

Abstract

In this study, Forcespinning® was used to produce nanofibers composed of Opuntia cochenillifera, “nopal,” mucilage (N) extract, chitosan (CH), and pullulan (PL) (N/CH/PL). These nopal-incorporating nanofibers were examined for their ability to sustain adhesion and proliferation of mouse embryonic fibroblast (NIH 3T3) cells. After a 6-day incubation period, N/CH/PL nanofibers displayed robust cell proliferation, with continued cell growth after an extended incubation period of 14 days. These results demonstrate that natural bioactive compounds can be combined with biodegradable polymers to provide an enhanced environment for cell growth, suggesting potential natural active ingredients as alternatives in wound dressings.

Engineering and Evaluation of Forcespun Gelatin Nanofibers as an Isorhamnetin Glycosides Delivery System

“…Annatto extract-loaded cellulose nanofibers did not demonstrate skin irritability or cytotoxicity, and showed a modulatory effect in the inflammatory process, providing evidence of the potentialities of this material for wound healing [13]. Scaffolds fabricated by the combination of Opuntia cochenillifera mucilage extract with natural biopolymers such as chitosan and pullulan showed an important increase in proliferation of mouse embryonic fibroblast (NIH 3T3) cells after 6 days of incubation, suggesting the potential of this composite to develop novel alternatives for wound dressing products [48].…”