Teste LERCAFÉ para sementes de cafeeiro com diferentes teores de água

Zonta, João Batista; Araújo, Eduardo Fontes; Araújo, Roberto Fontes; Reis, Múcio Silva; Zonta, Flávia Miranda Garcia

doi:10.1590/s0101-31222010000100002

Cited by 3 publications

(5 citation statements)

References 6 publications

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“…The results showed that increasing the immersion period, associated to the increase of the active chlorine concentration, cause excessive staining of the endosperm, precluding visual assessment by LERCAFE test. Zonta et al (2010) also failed to achieve satisfactory results for LERCAFE test when sodium hypochlorite solution with high levels of active chlorine combined with prolonged immersion periods was used. They reported that the test underestimated the results of coffee seeds germination under these conditions.…”

Section: Experiments 2: Adequacy Of the Lercafe Test Methodology With mentioning

confidence: 98%

“…The coffee seeds immersed for 2 h in a sodium hypochlorite solution containing 2.5% of active chlorine, did not have greenish coloration in the endosperm, precluding their assessment (Table 2). Zonta et al (2010) when testing the efficiency of LERCAFE test, failed to achieve satisfactory results for the treatments in which the coffee seeds were immersed in a sodium hypochlorite solution containing 2.5% of active chlorine for the periods of 1 and 2 h, because there was absence of staining for these treatments. For treatments of 3.5% of active chlorine for 6 h, 5 and 6% of active chlorine for 2, 3 and 6 h, an intense dark green color was observed, occupying a large part of the seeds´ endosperm, this complicated their assessment by LERCAFE test, and data were not computed.…”

Section: Experiments 1: Adequacy Of the Lercafe Test Methodologymentioning

confidence: 99%

“…From the evaluation of the demarcated region location, it is possible to sort the seeds as viable or non-viable (Reis et al, 2010). Reis et al (2010) concluded that treatment in which seeds were immersed in a sodium hypochlorite solution with 2.5% of active chlorine for a period of 3 h at 25°C is efficient in the estimation of the viability by the test, as Zonta et al (2010) concluded that the treatments of 2.5% of active chlorine with immersion time of 3 h at 35°C and 3.5% of active chlorine with immersion period of 2 h at 30°C are also efficient in estimating the viability by the test. Zonta et al (2008) used the LERCAFE test to estimate germination and to characterize damage in coffee seeds.…”

Section: Introductionmentioning

confidence: 99%

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Viability and enzyme activity of coffee seeds subjected to LERCAFE test

Nascimento¹,

Ribeiro²,

Nery³

et al. 2016

Afr. J. Agric. Res.

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The LERCAFE test consists in immersing coffee seeds in an active chlorine solution, which reacts with the seeds' endosperm, thus staining the viable parts dark green. The goal was to adapt the LERCAFE methodology to coffee seeds and assess the isoenzymatic profile of seeds subjected to the test. Two experiments were conducted, the first with adequacy of the LERCAFE test methodology, and the second experiment adequacy of the LERCAFE test methodology with the content of active chlorine quantified. A completely randomized design was used, with four replications of 50 seeds in a factorial scheme 4x4 (4 cultivars and 4 treatments of LERCAFE test). In the first experiment, it was possible to sort the cultivars into two quality levels by means of the treatments at 2.5% for 3 h, 3.5% for 2 h and 3 h. In the second experiment, it was found that the test enables determining the coffee seeds' physiological potential by using 2 and 3% active chlorine for 5 and 3 h, respectively. The coffee seeds subjected to LERCAFE test show changes in the activity of esterase, malate dehydrogenase, superoxide dismutase, catalase and alcohol dehydrogenase enzymes, and the activation or deactivation of these enzyme systems vary with the concentration and immersion time in the solution of active chlorine.

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Section: Experiments 2: Adequacy Of the Lercafe Test Methodology With mentioning

confidence: 98%

Section: Experiments 1: Adequacy Of the Lercafe Test Methodologymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Viability and enzyme activity of coffee seeds subjected to LERCAFE test

Nascimento¹,

Ribeiro²,

Nery³

et al. 2016

Afr. J. Agric. Res.

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show abstract

“…Delayed germination of seeds considerably extends the period of seedling formation process and may be attributed to properties of the seed cell walls and tissues, as well as to physical aspects such as endosperm hardness (Ferreira et al, 2018). The tissues surrounding the endosperm (micropylar endosperm) must be degraded (Zonta et al, 2010) so the radicle can then protrude and germination take place.…”

Section: Introductionmentioning

confidence: 99%

Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds

Oliveira

Souza

Silva

et al. 2020

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The endosperm of coffee beans is an important structure which is composed of different reserve compounds. In the present study, we followed anatomical features during the reserve mobilization of Coffea arabica L. cv 'Catuaí' seed samples, harvested at 20 different sites, after 0, 3, 6, 12, 24, 30 and 45 days of imbibition. Seed samples were properly stored for enzyme activity assessment and fixed in FAA solution (formaldehyde, acetic acid and ethanol, 1; 1; 18) and kept in ethanol 70% solution, for histochemical analysis. Alternatively, samples for phenolic compounds histochemical test were fixed in Ferrous sulfate in formalin. Fixed samples were cross-sectioned for detection of starch, lipids, essential oils, oleoresins, proteins, phenolic compounds, neutral polysaccharides, alkaloids, β-1,3 and β-1,4 glucans, and lignin. Overall, based on histochemical tests and enzyme activity, seed reserve mobilization was similar among the evaluated altitudes and mountainsides. During soaking, there is an intense consumption of reserve compounds, starting at the region close to the embryo. Reserve mobilization intensifies after root protrusion, from 12 days of soaking. The intensification of the reserve consumption concomitant with an increased activity of MDH is observed at the first 12 days, whereas SOD showed higher expression after 30 days of soaking. At the 30 th day, little reserve is observed in the endosperm. At 45 th day, cotyledon leaves are expanded, and the root system, constituted by the taproot and lateral roots, is well established.

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“…The reduction of time to germination test in the laboratories would be favorable for the production, commercialization, utilization and fiscalization of seeds (Guimarães et al, 2013). Tests such as the LERCAFE, which provides results in a relatively short period of time, are in the highest demand because they facilitate decision making at the different steps of the production process (Reis, Araújo, Dias, Sediyamam, & Meireles, 2010;Zonta, Araújo, Araújo, Reis, & Zonta, 2010).…”

Section: Introductionmentioning

confidence: 99%

<b>Image analysis of coffee seeds submitted to the LERCAFE test

et al. 2016

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ABSTRACT. The aim of this experiment was to quantify the stained areas of coffee seeds submitted to the LERCAFE test using image analysis. The seeds used were of the Catuaí Vermelho IAC 99 and Paradise cultivars. The physiological quality of the lots was assessed using germination tests, moisture content and a germination speed index. The LERCAFE test was conducted using seeds without parchment immersed in a solution of 3% sodium hypochlorite for 3 hours. Color photographs (RGB) with a resolution of 5 MPx were taken of each seed. The seeds were visually evaluated, and the functions generated from the analyses of the stained and non-stained regions were quantified by the Matlab R2009b program. Classification models were developed based on the Fisher Linear Discriminant Function and the evaluation of the adequacy of the models confusion matrix between the visual references and the classification generated by the linear functions. The image analysis for the creation of Fisher's linear discriminant function in the development of classifiers for the coffee seeds submitted to the LERCAFE test is potentially efficient; however, it remains necessary to test other discriminant functions and quantification methodologies.Keywords: determinants functions, sodium hypochlorite, pixels. Análise de imagens de sementes de café submetidas ao Teste LERCAFÉRESUMO. Objetivou-se com essa pesquisa quantificar as áreas coradas de sementes de café submetidas ao teste LERCAFÉ por meio da análise de imagens. Foram utilizadas sementes das cultivares Catuaí Vermelho IAC 99 e Paraíso. Foi realizada a caracterização da qualidade fisiológica dos lotes com os testes de germinação, umidade e índice de velocidade de germinação. O teste LERCAFÉ foi conduzido utilizando semente sem o pergaminho com solução de hipoclorito de sódio 3% pelo período de 3 horas. Foram feitas fotografias coloridas (RGB) com resolução de 5 MPx de cada semente. As sementes foram avaliadas visualmente e por funções geradas a partir da análise de regiões coradas e não coradas quantificadas pelo programa Matlab R2009b. Foram desenvolvidos modelos de classificação com base na Função Linear de Fisher e avaliação da adequação dos modelos pela Matriz de Confusão entre as referências visuais e classificação gerada pelas funções lineares. A análise de imagem para criação da função discriminante linear de Fisher no desenvolvimento de classificadores para sementes de café submetidas ao teste LERCAFÉ é potencialmente eficiente sendo necessário testar outras funções discriminantes e metodologias para quantificação.Palavras-chave: funções determinantes, hipoclorito de sódio, pixels.

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Teste LERCAFÉ para sementes de cafeeiro com diferentes teores de água

Cited by 3 publications

References 6 publications

Viability and enzyme activity of coffee seeds subjected to LERCAFE test

Viability and enzyme activity of coffee seeds subjected to LERCAFE test

Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds

<b>Image analysis of coffee seeds submitted to the LERCAFE test

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