Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds

Oliveira, Leonardo Araújo; Souza, Genaína Aparecida de; Silva, Bruno Tavares; Rocha, Antônio Augusto Gomes; Picoli, Edgard Augusto de Toledo; Pereira, Diego de Souza; Donzeles, Sérgio Maurício Lopes; Ribeiro, Marcelo de Freitas; Ferreira, Williams Pinto Marques

doi:10.25186/.v15i.1704

Cited by 4 publications

(8 citation statements)

References 52 publications

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“…These authors identified ultrastructural modifications in the cotyledon epidermis correlated with haustorial function. Studies with species from other botanical families relate a similar pattern of reserve degradation through histochemical analysis (Carvalho et al, 2022;Ferreira et al, 2020;Mazzottini-dos-Santos et al, 2017;Oliveira et al, 2020) and biochemical quantifications (Carvalho et al, 2022;Mello et al, 2022;Bicalho et al, 2016). The values of starch and protein content found in this study are similar to those reported during the formation of V. reitzii seedlings (Corredor-Prado et al, 2016.…”

Section: Discussionsupporting

confidence: 80%

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

2023

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Studies on the germination and establishment of plants are key pieces to understanding the reproductive success of plants. This work aimed to describe in vitro germination and reserve mobilization in the bromeliad Vriesea friburgensis through morphological, histochemical, and biochemical analysis. The conditions used in this study for the in vitro germination are adequate. From the third day of in vitro inoculation, a uniform germination of 98% was obtained, exhibiting a high physiological quality of the seeds and a high potential to produce seedlings (94%). There is early reserve mobilization, which began in the imbibition phase. The accumulated reserves in the endosperm cytoplasm are degraded by hydrolytic enzymes provided by the aleurone layer. It is possible that compounds in the cell walls of the endosperm contribute to a lesser extent in mobilization. Additionally, it was observed that starch accumulation in the cotyledon increases when the seedling has formed. Results from this study provide insights for future studies on ecology, seed technology, and conservation in this species. This study contributes to the limited knowledge of the dynamics of reserves during germination and seedling establishment in Bromeliaceae. To the best of our knowledge, this is the first study with this approach in the genus Vriesea.

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Section: Discussionsupporting

confidence: 80%

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

2023

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“…However, it is important to note that seeds harvested at immature stages may have slow and irregular germination. It may be due to the presence of fruits that are not fully formed, and with insufficient accumulation of reserves (Oliveira et al 2020). Variability also depends on seed size, physical quality, seed health, storage time, and materials (Anteneh et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Physiological quality of seeds of Coffea canephora from early and late clones during maturation

Crasque,

Brandão,

Cerri Neto

et al. 2024

Bragantia

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The objective of this study was to evaluate the physiological quality of seeds from early and late maturing Coffea canephora clones, aiming to identify the ideal harvest time. The fruits were collected every two weeks from 188 days after anthesis (DAA) and characterized as green, cane green, cherry, raisin and dry. The seeds were evaluated for water content, germination percentage, normal and abnormal seedlings, nongerminated seeds and dead seeds, vigor index, hypocotyl lenght, and root length. Seed germination of the early maturing clone started at 202 DAA, while that of the late maturing clone started at 230 DAA, with both clones showing a water content of 63% and a dry mass of 37%. Although the optimum harvest point was identified at the cherry ripening stage, it was found that the late maturing clone showed a more pronounced variation in the maturation process, directly impacting the physiological quality of the seeds. Furthermore, a significant correlation was observed between the maturation stages and the physiological quality of the seeds. For the early maturing clone, the maximum physiological quality was recorded at 244 DAA, corresponding to 80% of fruits at the cherry stage, while for the late maturing clone this point was reached at 326 DAA, with 98% of the fruits ripe.

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“…Our biochemical analyses confirmed that MLG is the main component of the cell wall to be degraded and it is reasonable to attribute the thinning of the endosperm cell wall to this mechanism. Again, thinning of cell walls throughout germination has been observed in many plants, such as in barley grains and coffee beans [ 41 , 42 ]. Opanowicz et al estimated that the cell wall thickness in the central endosperm of Brachypodium grain was about 4.4 µm, which is more than twice the thickness of the cell walls in the central endosperm of wheat grain [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Mixed-Linkage Glucan Is the Main Carbohydrate Source and Starch Is an Alternative Source during Brachypodium Grain Germination

Francin-Allami

Bouder

Geairon

et al. 2023

IJMS

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Seeds of the model grass Brachypodium distachyon are unusual because they contain very little starch and high levels of mixed-linkage glucan (MLG) accumulated in thick cell walls. It was suggested that MLG might supplement starch as a storage carbohydrate and may be mobilised during germination. In this work, we observed massive degradation of MLG during germination in both endosperm and nucellar epidermis. The enzymes responsible for the MLG degradation were identified in germinated grains and characterized using heterologous expression. By using mutants targeting MLG biosynthesis genes, we showed that the expression level of genes coding for MLG and starch-degrading enzymes was modified in the germinated grains of knocked-out cslf6 mutants depleted in MLG but with higher starch content. Our results suggest a substrate-dependent regulation of the storage sugars during germination. These overall results demonstrated the function of MLG as the main carbohydrate source during germination of Brachypodium grain. More astonishingly, cslf6 Brachypodium mutants are able to adapt their metabolism to the lack of MLG by modifying the energy source for germination and the expression of genes dedicated for its use.

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Histochemical approach of the mobilization of reserve compounds in germinating coffee seeds

Cited by 4 publications

References 52 publications

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

In vitro germination and reserve mobilization of Vriesea friburgensis Mez

Physiological quality of seeds of Coffea canephora from early and late clones during maturation

Mixed-Linkage Glucan Is the Main Carbohydrate Source and Starch Is an Alternative Source during Brachypodium Grain Germination

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