Temporal biological nitrogen fixation pattern in soybean inoculated with <i>Bradyrhizobium</i>

Arachchige, Pavithra S. Pitumpe; Rosso, Luiz H. Moro; Hansel, Fernando Dubou; Ramundo, B. A.; Asebedo, R.; Ciampitti, Ignacio A.; Jagadish, S. V. Krishna

doi:10.1002/agg2.20079

Cited by 15 publications

(12 citation statements)

References 37 publications

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“…Nitrogen fixation in soybean can vary depending on the growth stages of the plant. In general, SNF is lowest during the vegetative stage, then rapidly increases between R1 and R3 growth stages, before peaking then dropping upon pod formation (post R5 stage) (Zapata et al, 1987;Pitumpe Arachchige et al, 2020). In soybean, a significant portion (59%) of seed N is derived from remobilization of vegetative N, which represents N accumulated from SNF and soil uptake at the R5.5 stage (Ortez et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Testing Whether Pre-Pod-Fill Symbiotic Nitrogen Fixation in Soybean Is Subject to Drift or Selection Over 100 Years of Soybean Breeding

et al. 2021

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Soybean [Glycine max (L.) Merr.] is the world's leading legume crop and the largest oilseed crop. It forms a symbiotic relationship with rhizobia bacteria residing in root nodules that provide fixed nitrogen to host plants through symbiotic nitrogen fixation (SNF). In soybean, it has been widely reported that the highest SNF occurs at the pod-filling stage, associated with the peak demand for nitrogen. However, the majority of seed nitrogen is derived from remobilizing root/shoot nitrogen, representing cumulative SNF from the seedling stage to the pre-pod-fill stage. Therefore, the question arises as to whether there has also been selection for improved SNF at these earlier stages, or whether pre-pod-fill SNF traits have drifted. To test this hypothesis, in this study, pre-pod SNF-related traits were evaluated in soybean cultivars that span 100 years of breeding selection in the Canadian Province of Ontario. Specifically, we evaluated SNF traits in 19 pedigree-related historical cultivars and 25 modern cultivars derived from the University of Guelph soybean breeding program. Field trials were conducted at Woodstock, Ontario, Canada in 2016 and 2017, and various SNF-related traits were measured at pre-pod-fill stages (R1-R3), including nitrogen fixation capacity. Considerable variation was observed among Canadian soybean cultivars released over the past 100 years for pre-pod-fill nitrogen fixation. The modern soybean cultivars had similar or moderately higher pre-pod-fill SNF compared to the historical lines in terms of the percentage of nitrogen derived from the atmosphere (%Ndfa) and total shoot fixed nitrogen. These findings suggest that, despite no direct selection by breeders, pre-pod-fill nitrogen fixation, and associated SNF traits have been maintained and possibly improved in modern soybean breeding. However, the low level of pre-pod-fill SNF in some modern cultivars, and generally wide variation observed in SNF between them, suggest some level of genetic drift for this trait in some pedigrees. Specific historical and modern soybean cultivars were identified as potential parents to enable targeted breeding for improved pre-pod-fill SNF. This retrospective study sheds light on our understanding of the impact of decades of recent selective breeding on pre-pod-fill nitrogen fixation traits in soybean in a temperate environment.

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Section: Introductionmentioning

confidence: 99%

Testing Whether Pre-Pod-Fill Symbiotic Nitrogen Fixation in Soybean Is Subject to Drift or Selection Over 100 Years of Soybean Breeding

et al. 2021

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“…Diverse studies conducted to evaluate the efficiency of BNF have focused on characteristics related to N assimilation capacity by the plant and to nodulation components (Arachchige et al., 2020; Grunvald et al., 2018; Hwang et al., 2014). Thus, to perform the partial diallel study, the traits N and chlorophyll contents, number of nodules, DWS, and dry weight of nodules, as well as the Z‐index, were considered.…”

Section: Resultsmentioning

confidence: 99%

Combining abilities of soybean progenies and bradyrhizobium strains for biological nitrogen fixation

et al. 2023

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We aim to study the biological nitrogen fixation (BNF) ability of soybean (Glycine max) progenies and their ability for symbioses using different Bradyrhizobium strains, as well as select progenies and strains that are more efficient in BNF to guide strategies in soybean breeding. An experiment was conducted in a greenhouse, evaluating 24 soybean progenies and five Bradyrhizobium strains. Mean values from analysis of variance were compared by the Scott-Knot test, and partial diallel analysis was performed to estimate general and specific combining ability. Following analysis of the greenhouse experiment, 10 soybean progenies and two Bradyrhizobium strains (SEMIA 587 and INPA 03-11B) were selected to carry out a field trial experiment.Biological nitrogen fixation was affected by genotype, strain, and genotype × strain interactions. Additive effects were predominant on the traits related to BNF, indicating that recurrent selection could be an effective breeding strategy. The progenies showed higher values for the traits related to field nodulation but did not improve seed yield. The EA-8 progeny stood out for high yield and had good results in traits related to BNF and early maturity. The INPA 03-11B strain had the best result for seed yield, indicating high potential for use as an inoculant in soybean crops.

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“…These results suggest the impact of variation in the total count and individual nodule area on the total nodule area among these genotypes. It has been shown that nodule count and biological nitrogen fixation can have a strong relationship (Pitumpe Arachchige et al, 2020), suggesting that the ability to screen genotypes for nodule count can have a relational impact on nitrogen fixation. The size of nodules is also directly related to biological nitrogen fixation, as shown in Brun (Lawn & Brun, 1974).…”

Section: Discussionmentioning

confidence: 99%

Using Machine Learning Enabled Phenotyping To Characterize Nodulation In Three Early Vegetative Stages In Soybean

Carley

Zubrod

Dutta

et al. 2022

Preprint

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The symbiotic relationship between soybean [Glycine max L. (Merr.)] roots and bacteria (Bradyrhizobium japonicum) lead to the development of nodules, important legume root structures where atmospheric nitrogen (N2) is fixed into bio-available ammonia (NH3) for plant growth and development. With the recent development of the Soybean Nodule Acquisition Pipeline (SNAP), nodules can more easily be quantified and evaluated for genetic diversity and growth patterns across unique soybean root system architectures. We explored six diverse soybean genotypes across three field year combinations in three early vegetative stages of development and report the unique relationships between soybean nodules in the taproot and non-taproot growth zones of diverse root system architectures of these genotypes. We found unique growth patterns in the nodules of taproots showing genotypic differences in how nodules grew in count, size, and total nodule area per genotype compared to non-taproot nodules. We propose that nodulation should be defined as a function of both nodule count and individual nodule area resulting in a total nodule area per root or growth regions of the root. We also report on the relationships between the nodules and total nitrogen in the seed at maturity, finding a strong correlation between the taproot nodules and final seed nitrogen at maturity. The applications of these findings could lead to an enhanced understanding of the plant-Bradyrhizobium relationship, and exploring these relationships could lead to leveraging greater nitrogen use efficiency and nodulation carbon to nitrogen production efficiency across the soybean germplasm.

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Temporal biological nitrogen fixation pattern in soybean inoculated with Bradyrhizobium

Cited by 15 publications

References 37 publications

Testing Whether Pre-Pod-Fill Symbiotic Nitrogen Fixation in Soybean Is Subject to Drift or Selection Over 100 Years of Soybean Breeding

Testing Whether Pre-Pod-Fill Symbiotic Nitrogen Fixation in Soybean Is Subject to Drift or Selection Over 100 Years of Soybean Breeding

Combining abilities of soybean progenies and bradyrhizobium strains for biological nitrogen fixation

Using Machine Learning Enabled Phenotyping To Characterize Nodulation In Three Early Vegetative Stages In Soybean

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