1990
DOI: 10.1590/s0074-02761990000100010
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Abstract: A simple and rapid staphylococcal coagglutination test for the detection of Toxoplasma gondii antigens in mice urine is described. A suspension of protein-A containing Staphylococcus aureus coated with rabbit hyperimmune serum was used as reagent. The sensitivity of the antigen assay was found to be at least 118 ng of the antigen protein per ml. No coagglutination was observed when the reagent was challenged against antigenic solutions of other parasites. The suitability of the method for detecting antigens of… Show more

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Cited by 5 publications
(3 citation statements)
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“…This was more pronounced in compounds 4a and 6. The parasite count results were in accordance with the results obtained from the Co-A test, which served as the best means of diagnosis of recent Toxoplasma infection, and considered a marker of cure (Fachado et al, 1990;Raizman and Neva, 1975). In the present study, no clumping was visible for compounds 4a and 6.…”
Section: Discussionsupporting
confidence: 89%
“…This was more pronounced in compounds 4a and 6. The parasite count results were in accordance with the results obtained from the Co-A test, which served as the best means of diagnosis of recent Toxoplasma infection, and considered a marker of cure (Fachado et al, 1990;Raizman and Neva, 1975). In the present study, no clumping was visible for compounds 4a and 6.…”
Section: Discussionsupporting
confidence: 89%
“…Já foi demonstrado que o coelho mostra-se um bom modelo experimental para o estudo da toxoplasmose (FACHADO et al, 1990;HITT e FILICE, 1992;WATANABE et al,1993;BLACKWELL et al, 1994;PEIFFER et al, 1994;HUNTER et al, 1995), porém * Avaliação variando de: + = pouco freqüente / +++++ = 100% de freqüência ** Grupo I: Inoculado *** Grupo II: Não inoculado (controle) Os sinais clínicos observados, como hipertermia, estertores pulmonares, apatia, taquipnéia e secreção Tabela 6 -Freqüência de alterações microscópicas encontradas em exames histopatológicos das coelhas pertencentes aos grupos inoculados com 1 x 10 4 oocistos de T. gondii (n=9) e não inoculado (n=3). Sharma et al (1973).…”
Section: Discussionunclassified
“…Recently, some researchers have described the use of T. gondii DNA sequence for the diagnosis of toxoplasmosis using the polymerase chain reaction (PCR) (Dupcuy-Camet et al 1993). We have reported the use of coagglutination assay to detect T. gondii antigens in the urine of infected mice (Fachado et al 1990) and also in the urine of AIDS patients (Fachado et al 1992 We have developed the coagglutination technique using urine of AIDS patients with acute cerebral toxoplasmosis. In order to confirm the toxoplasmic origin of the antigens detected by CoAToxo, the immunoblot was employed.…”
mentioning
confidence: 99%