Tannic acid‐stabilized pericardium tissue: IR spectroscopy, atomic force microscopy, and dielectric spectroscopy investigations

Abstract

Infrared (IR) spectroscopy, atomic force microscopy (AFM), and dielectric spectroscopy methods were employed to study structural and dynamic changes in the tannic acid (TA)-stabilized pericardium tissue. Chemically stabilized pericardium tissue is widely used in construction of the tissue derived bioprostheses. IR spectra recorded in the range 400-4000 cm-1 allowed us to recognize different types of TA-collagen interactions. Formation of hydrogen bonds between amine as well as amide NH groups from collagen and hydroxyl groups of TA was analyzed. The AFM imaging showed that the stabilization procedure with TA introduces considerable changes in both surface topography and thickness of collagen fibrils as well as in fibril arrangement on the tissue surface. It was found, that these structural changes have an impact on the dielectric behavior of the TA-stabilized tissue. The dielectric spectra for the native and TA-stabilized tissues were measured in the frequency and temperature ranges of 10(-1) -10(7) Hz and 120-270 K, respectively. The dielectric spectra revealed the relaxation process due to orientation of bound water supplemented by the fluctuation of collagen polar side groups. At the temperatures above approximately 210 K, the relaxation due to ion migration process was observed. It was found that both relaxation processes were influenced by the TA-collagen interaction.

Mechanical Properties of Tannic-acid-treated Dentin Matrix

“…TA have been reported (Koide and Daito, 1997;Isenburg et al, 2006). Structural and dynamic changes in TA-collagen interactions have been characterized by infra-red spectroscopy in TA-stabilized pericardial tissue (Jastrzebska et al, 2006). Hydrogen bonds are formed between amide NH groups from collagen and hydroxyl groups from TA (Jastrzebska et al, 2006).…”

“…Structural and dynamic changes in TA-collagen interactions have been characterized by infra-red spectroscopy in TA-stabilized pericardial tissue (Jastrzebska et al, 2006). Hydrogen bonds are formed between amide NH groups from collagen and hydroxyl groups from TA (Jastrzebska et al, 2006). It can be expected that changes to dentin matrix would be similar to other type I collagen-based tissues; therefore, the formation of hydrogen bonds by TA is most likely accountable for the changes to dentin modulus of elasticity.…”

“…Several synthetic and natural chemicals have the ability to increase the number of covalent inter-and intra-molecular collagen cross-links (Sung et al, 1999;Han et al, 2003) and affect its properties. Tannic acid, a commercial form of condensed tannin, is a naturally occurring polyphenol with weak acidity that has the ability to modify collagen chemically (Jastrzebska et al, 2006).…”

Pericardial Processing: Challenges, Outcomes and Future Prospects

“…Reuterin cross-linked pericardium exhibits comparable results to glutaraldehyde in terms of resistance against enzymatic degradation, denaturation temperature and free amino group content, while decreasing cytotoxic effects (Chen et al, 2002). Tannic acid has been studied on pericardial tissue and was shown to crosslink proteins by creating multiple hydrogen bonds due to its hydroxyl groups (Cwalina et al, 2005;Jastrzebska et al, 2006). It exerts an anti-inflammatory effect, especially on macrophages, as well as an anti-calcification effect on glutaraldehyde-fixed bovine pericardium (Wang et al, 2008).…”

Effects of laser irradiation on collagen organization in chemically induced degenerative annulus fibrosus of lumbar intervertebral disc

“…Cross-links are thought to push adjacent molecules apart in the closely packed overlap regions of the fibrils and to cause the expansion of the collagen network [54]. The increase in the intermolecular spacing was seen with X-ray diffraction and the disturbance of the molecular arrangement of collagen fiber and fibril broadening was seen in the section of atomic force microscopy images after the incubation of connective tissues with sugars [54][55][56]. Glycation results in alterations in tissues morphology.…”