Mechanical Properties of Tannic-acid-treated Dentin Matrix

Abstract

Dentin collagen is a major component of the hybrid layer, and its stability may have a great impact on the properties of adhesive interfaces. We tested the hypothesis that the use of tannic acid (TA), a collagen cross-linking agent, may affect the mechanical properties and stability of the dentin matrix. The present study evaluated the effects of different concentrations of TA on the modulus of elasticity and enzymatic degradation of dentin matrix. Hence, the effect of TA pre-treatment on resin-dentin bond strength was assessed with the use of two bonding systems. Sound human molars were used and prepared according to each experimental design. The use of TA affected the properties of demineralized dentin by increasing its stiffness. TA treatment inhi bited the effect of collagenase digestion on dentin matrix, particularly for 10%TA and 20%TA. The TA-dentin matrix complex resulted in improved bond strength for both adhesive systems.

Effect of Tannic Acid on Bond Strength of Etch and Rinse and Self-etch Adhesive Systems in Dentin of Primary Teeth

“…20 Extended time treatment with TA might protect the collagen structures against the attack by phosphoric acid. 9 Perhaps, despite our findings, if dentin is exposed to TA for a long duration, it would cause a significant elevation in the immediate bond strength of the SB adhesive. It has been noticed that using TA for about 1 hour is not practical in clinic and is done merely in laboratory studies.…”

“…They concluded that TA could cause excess of the dentinal stiffness. 9 Also, their study showed that TA (20%) could increase microtensile bond strength of adhesive SB. However, our findings indicated that TA reduced the SB strength and did not affect instant stability of CSB.…”

“…This bond would increase stability of the collagen fibers and elevate their resistance against dentin matrix metalloproteinases (MMPs). 9 Therefore, due to lack of such studies in this field and by considering the importance of this issue, the present study aimed to investigate the effect of TA on bond strength of etch and rinse and self-etch adhesive systems in the dentin of primary teeth.…”

Studies on collagen-tannic acid-collagenase ternary system: Inhibition of collagenase against collagenolytic degradation of extracellular matrix component of collagen

“…It is known to form multiple hydrogen bonds with proteins, particularly those rich in proline such as elastin and collagen and used in the manufacture of gelatin product and leather adhesives 3,4 . It has been suggested as a potential crosslinking agent and improves the elastin and collagen stability in cardiovascular implants as evidenced by an increased resistance to proteolytic degradation against collagenase activity 5,6 . The inhibition of MMPs caused by plant polyphenolics was confirmed by gelatin zymography and observed for MMPs associated with various connective tissues 7,8 .…”

Aumento de longevidad de restauraciones de resinas compuestas y de su unión adhesiva. Revisión de tema

“…PA es un potente antioxidate y agente de entrecruzamiento, de baja toxicidad. Se ha demostrado que PA proveniente de extractos de las semillas de la uva, mejoran la resistencia traccional y la rigidez del colágeno dentinario 151,153,154 y la estabilidad a largo plazo de la matriz de colágeno de la dentina. 155,156 Además de su efecto de entrecruzamiento, la PA obtenida del olmo y extractos de arán-danos, también ha demostrado inhibir la producción de MMPs 1, 3, 7, 8, 9 y 13 de los macrófagos e inhibir la actividad catalítica de MMP 1 y 9.…”