Pericardial Processing: Challenges, Outcomes and Future Prospects

Rémi, Escande; Khelil, Nizar; Centa, Isabelle Di; Roques, Caroline; Ba, Maguette; Medjahed-Hamidi, Fatima; Chaubet, Frédéric; Letourneur, Didier; Lansac, Emmanuel; Meddahi‐Pellé, Anne

doi:10.5772/24949

Cited by 25 publications

(32 citation statements)

References 129 publications

(100 reference statements)

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“…Also, decellularization chemistry has shown to change the tissue molecular structure and affect mass transferring and cell response (Moore, Sarntinoranont, & Mcfetridge, ). The risk of residual chemicals is another problem with decellularization; for example, SDS has a high affinity for proteins (Grefrath & Reynolds, ), eliminates ECM growth factors (Reing et al, ), and leads to incomplete decellularization (Mendoza‐Novelo & Cauich‐Rodríguez, ; Rémi et al, ), ECM retention (Arai & Orton, ; Kawazoye et al, ; Knight, Wilcox, Korossis, Fisher, & INgham, ), and calcification (Jorge‐Herrero et al, ). Demolition of ECM components will result in deterioration of the mechanical properties of the tissue (Elder et al, ).…”

Section: Discussionmentioning

confidence: 99%

A comparison study of different decellularization treatments on bovine articular cartilage

Ghassemi

Saghatoleslami

Mahdavi-Shahri

et al. 2019

J Tissue Eng Regen Med

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Previous researches have emphasized on suitability of decellularized tissues for regenerative applications. The decellularization of cartilage tissue has always been a challenge as the final product must be balanced in both immunogenic residue and mechanical properties. This study was designed to compare and optimize the efficacy of the most common chemical decellularization treatments on articular cartilage. Freeze/thaw cycles, trypsin, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), and Triton‐X 100 were used at various concentrations and time durations for decellularization of bovine distal femoral joint cartilage samples. Histological staining, scanning electron microscopy, DNA quantification, compressive strength test, and Fourier‐transform infrared spectroscopy were performed for evaluation of the decellularized cartilage samples. Treatment with 0.05% trypsin/EDTA for 1 day followed by 3% SDS for 2 days and 3% Triton X‐100 for another 2 days resulted in significant reduction in DNA content and simultaneous maintenance of mechanical properties. Seeding the human adipose‐derived stem cells onto the decellularized cartilage confirmed its biocompatibility. According to our findings, an optimized physiochemical decellularization method can yield in a nonimmunogenic biomechanically compatible decellularized tissue for cartilage regeneration application.

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Section: Discussionmentioning

confidence: 99%

A comparison study of different decellularization treatments on bovine articular cartilage

Ghassemi

Saghatoleslami

Mahdavi-Shahri

et al. 2019

J Tissue Eng Regen Med

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“…69 Other fixation methods, including photooxidization and decellularization, have been considered, although the long-term effects of these treatments still need to be determined. 70,71 There are two main causes of bioprosthetic valve failure; mechanical and chemical influences. Pericardium and xenografts are prone to calcification, particularly in regions exposed to high stress, which leads to eventual stiffening of the leaflets.…”

Section: Bioprosthetic Valvesmentioning

confidence: 99%

The aortic valve: structure, complications and implications for transcatheter aortic valve replacement

2014

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The aortic valve operates in a complex haemodynamic environment, opening and closing over 100,000 times a day. When complications arise, such as aortic stenosis, prognosis can be very poor, leading to death within the first few years. Surgical valve replacement is currently the standard treatment for aortic stenosis. A thorough understanding of the anatomy and function of the native valve is imperative when developing a prosthetic replacement that can withstand the complex demands of the heart. This review focuses on the anatomy, structure and disease of the aortic valve and the implications for a transcatheter aortic valve replacement (TAVR). Current complications with TAVR, such as major vascular bleeding, conduction disturbances and patient-prosthesis mismatch (PPM), can be overcome by reducing the delivery profile and through the use of more accurate imaging technologies to work towards a fully functional and durable prosthesis.

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“…This tissue is a durable, thin sheet of collagen fibre‐reinforced matrix that has been extensively used in the manufacture of medical devices, including bioprosthetic heart valves and tendon grafts. The pericardium itself is comprised of both dense regular and irregular connective tissue (predominantly type I collagen) that is organized into fibrils, fibres, fibre bundles and laminates (Rémi et al ., ). The fibrous pericardium is the outermost layer of the pericardium containing aligned type I collagen fibres, which is fused to an adjacent layer of parietal pericardium containing a multi‐directional network of fine collagen fibres and elastin.…”

Section: Introductionmentioning

confidence: 97%

The fabrication and characterization of a multi-laminate, angle-ply collagen patch for annulus fibrosus repair

McGuire

Borem

Mercuri

2016

J Tissue Eng Regen Med

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One major limitation of intervertebral disc (IVD) repair is that no ideal biomaterial has been developed that effectively mimics the angle-ply collagen architecture and mechanical properties of the native annulus fibrosus (AF). Furthermore, it would be beneficial to devise a simple, scalable process by which to manufacture a biomimetic biomaterial that could function as a mechanical repair patch to be secured over a large defect in the outer AF that will support AF tissue regeneration. Such a biomaterial would: (1) enable the employment of early-stage interventional strategies to treat IVD degeneration (i.e. nucleus pulposus arthroplasty); (2) prevent IVD re-herniation in patients with large AF defects; and (3) serve as a platform to develop full-thickness AF and whole IVD tissue engineering strategies. Due to the innate collagen fibre alignment and mechanical strength of pericardium, a procedure was developed to assemble multi-laminate angle-ply AF patches derived from decellularized pericardial tissue. Patches were subsequently assessed histologically to confirm angle-ply microarchitecture, and mechanically assessed for biaxial burst strength and tensile properties. Additionally, patch cytocompatibility was evaluated following seeding with bovine AF cells. This study demonstrated the effective removal of porcine cell remnants from the pericardium, and the ability to reliably produce multi-laminate patches with angle-ply architecture using a simple assembly technique. Resultant patches demonstrated their inherent ability to resist biaxial burst pressures reminiscent of intradiscal pressures commonly borne by the AF, and exhibited tensile strength and modulus values reported for native human AF. Furthermore, the biomaterial supported AF cell viability, infiltration and proliferation.

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Pericardial Processing: Challenges, Outcomes and Future Prospects

Cited by 25 publications

References 129 publications

A comparison study of different decellularization treatments on bovine articular cartilage

A comparison study of different decellularization treatments on bovine articular cartilage

The aortic valve: structure, complications and implications for transcatheter aortic valve replacement

The fabrication and characterization of a multi-laminate, angle-ply collagen patch for annulus fibrosus repair

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