T-regulatory cells subpopulation in bronchial asthma and heterogeneous phenotypes of chronic obstructive pulmonary disease

Кириллова, Н. А.; Deyev, I. A.; Kremer, Ye. E.; Ogorodova, L. M.; Chernogoryuk, G. E.

doi:10.20538/1682-0363-2011-1-48-54

Cited by 4 publications

(5 citation statements)

References 16 publications

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“…The findings of the study are coherent with the reported information that chronic inflammatory process during bronchial asthma is accompanied with intensification of synthesis of biologically active substances which have an effect on the lymphocytes' receptor system, resulting in change of the level of their functional capacities [9,13].…”

Section: Resultssupporting

confidence: 88%

“…One of mechanisms of BA pathogenesis is imbalance of cytokines of various T-helper profiles, in particular, IFNγ and IL-4, which characterize the immune response polarization of Th1 and Th2 inflammation type [2,7]. IFNγ regulates the differentiation of naïve CD4 + -cells into Th1 and inhibits the proliferation of Th2 [7,9]. Average levels of spontaneous production of IFNγ and IL-4 in children with allergen-induced phenotype were equal to 8.07±1.42 pg/ml and 5.04±0.08 pg/ml, after mitogen induction -to 16.38±1.77 pg/ml and 13.15±1.21 pg/ml.…”

Section: Resultsmentioning

confidence: 99%

“…Pathogenetic significance of immune and cytokine mechanisms in allergic inflammation determine the information value and importance of monitoring of immunological indicators in the course of immunotropic therapy for bronchial asthma [3,7,9].…”

Section: Introductionmentioning

confidence: 99%

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Characteristics of Immunocompetent Cells in Children With Allergen-Induced Phenotype of Bronchial Asthma Treated With Glucoseminylmuramildipeptide

2021

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Purpose: to study the changes in quantitative values and functional activity of immunocompetent cells on application of glucoseminylmuramildipeptide in children with allergen-induced phenotype of bronchial asthma.We have performed an integrated assessment of parameters of innate and acquired immunity in 60 children at the age of 3-11 years old with allergen-induced bronchial asthma (BA) with mild clinical course of disease, and in 30 healthy children of the same age. BA phenotypes were verified in accordance with PRACTALL international consensus report (2008). Study exclusion criteria were: severe course of bronchial asthma and application of immunocorrecting therapy during preceding six months. We conducted a prospective parallel open study of the effect of glucoseminylmuramildipeptide on the parameters of immunocompetent cells during three months with division into two control groups by random sampling technique on the basis of therapy being performed. To analyze the venous blood cells, we used flow cytofluorometer COULTER EPICS XL by Beckman Coulter Inc. Cytokine levels were determined using immunoenzyme method with reagents by R&D Diagnostics Inc (USA) and IgE – with reagents by Alkor Bio Company (St. Petersburg), production of cytokines – using reagents by Vektor-Best (Novosibirsk). Statistical processing of data was performed using Statistica 10 program with significance level p < 0.05, assessment of correlations by Spearman correlation analysis, multidimensional correlation analysis with V.P. Terentyev’s method of correlation pleiades (1959) and testing for normal distribution of characteristic values (Shapiro–Wilk). The scope of our study permitted to evaluate its findings with accuracy 95-99%.The changes of the adaptive response system in children with allergen-induced phenotype of BA were characterized by the intensified proliferation, suppression of negative regulation processes, activation of synthesis of Th2-profile cytokines, and intensified synthesis of IgE.The identified impairments of availability, functional activity of immunocompetent cells and cytokine production were preserved in application of inhaled corticosteroids therapy, with further decreasing of IFNγ synthesis.Application of glucoseminylmuramildipeptide in children with BA provided for reduction of spontaneous and mitogen-induced production of IL-4 and correction of deviated structural and functional characteristics of immunocompetent cells.Incorporation of glucoseminylmuramildipeptide into therapeutic regimens for allergen-induced phenotype of BA in children promoted normalization of parameters of the cellular component of immune system, amelioration of Th1/Th2-imbalance, increase of Th1 activity, and adequate spontaneous and induced production of IFNγ by peripheral blood cells.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

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Characteristics of Immunocompetent Cells in Children With Allergen-Induced Phenotype of Bronchial Asthma Treated With Glucoseminylmuramildipeptide

2021

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“…Despite the variety of clinical symptoms of these diseases the development of inflammation is largely determined by immune system [ 3 – 6 ]. It is known that eosinophilic inflammation in asthma is controlled by CD4 + lymphocytes [ 7 , 8 ]. The disorders of respiratory function are occasional and reversible.…”

Section: Introductionmentioning

confidence: 99%

“…In COPD inflammation involves neutrophils, macrophages, and CD8 + lymphocytes. An important feature of COPD is a progressing pulmonary obstruction concurrent with airway remodeling [ 8 , 9 ]. Despite the differences in the development mechanisms of asthma and COPD, these diseases have some common features in clinical manifestations and prevention and treatment strategies [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

The Mechanisms of the Regulation of Immune Response in Patients with Comorbidity of Chronic Obstructive Pulmonary Disease and Asthma

Калинина

Denisenko

Виткина

et al. 2016

Canadian Respiratory Journal

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Background. Comorbidity of chronic obstructive pulmonary disease (COPD) and asthma (asthma COPD overlap syndrome, ACOS) is a significant problem in pulmonary practice, whose pathogenetic issues are not clarified yet. Objective. To study the features of the regulation of immune response in patients with comorbid COPD and asthma. Methods. We assessed the levels of CD3+, CD4+, CD8+, CD4+/CD8+, CD19+, CD25+, HLA-DR, total IgE, TNF-α, IL-4, IFN-γ, TXB2, and LTB4 in patients with comorbid COPD and asthma. Results. The study involved 44 people with COPD, 39 people with asthma, and 12 people with comorbid COPD and asthma. The specific features in comorbid COPD and asthma were lymphocytosis, increased absolute count of T-helper cells, increased cytotoxic T-lymphocytes in relative and absolute count, increased relative and absolute numbers of B-lymphocytes, and high levels of total IgE. The elevated levels of TNF-α and IL-4 and inhibition of IFN-γ production were detected. The content of LTB4 was maximal; TXB2 levels were higher than in control group but lower than in COPD and asthma. Conclusion. In comorbid COPD and asthma inflammation increased even during stable period. High levels of eicosanoids, low production of Th1-type cytokines, and active synthesis of opposition IL-4, along with increased IgE, indicate the activation of Th2-type immune response.

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CLINICAL AND IMMUNOLOGICAL COMPARISONS IN Th- DEPENDENT IMMUNE RESPONSE MECHANISMS AMONG PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

et al. 2018

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Chronic obstructive pulmonary disease (COPD) is a global public health problem. Studies in immunological features and their correlations with clinical course of COPD are of importance. The aim of this study was to elucidate clinical and immunological features in COPD of different severity grade, concerning Th1- and Тh17-dependent types of immune response.The study included 132 COPD patients and 32 healthy individuals. According to clinical and functional patterns, the patients with COPD were divided into 3 groups, i.e., 36 cases (28%) of mild severity; 62 individuals (48%), of moderate severity, and 30 patients (23%) of severe clinical grade. We have performed both clinical and immunological evaluation of the patients. The Th1- and Th17-specific lymphocyte subpopulations were assessed according to the serum levels of cytokines, i.e. tumor necrosis factor (TNFα), IL-4, IL-10, IL-17A, IL-21, IFNγ, as well as transforming growth factor β1 (TGF-β1). We have also determined expression of IL-6R receptor (CD126+) on mature T lymphocytes (CD3+) and T helper cells (CD4+) from peripheral blood. We have obtained the following results: the patients with mild-grade COPD exhibited three different T cell phenotypes were determined, with a prevalence of Th1-dependent immune response. The IL-6R were mostly expressed on CD3+CD126+ cells for the Th1/Th17 phenotype, and CD4+CD126+ cells in cases of Th17-dependent type immune response. In patients with COPD of moderate severity, the Th1, Th17, or Th1/Th17 types of immune response was revealed at similar rates. The level of IL-6R expression on mature T lymphocytes and T-helper cells increased to the greatest extent in cases of Th17-dependent immune response. In severe COPD patients, we have found a dominance of Th17 and Th1/Th17 type immune response. The levels of IL-6R expressionwere increased in Th17- and Th1/Th17-dependent types of immune response, the most significant increase was observed for CD4+ cells in Th17 phenotype. Clinical features of COPD proved to be associated with the phenotypes of immune response. These results allow of specifying the inflammatory phenotype, predicting the course of chronic disease, and selecting appropriate therapy.

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T-regulatory cells subpopulation in bronchial asthma and heterogeneous phenotypes of chronic obstructive pulmonary disease

Cited by 4 publications

References 16 publications

Characteristics of Immunocompetent Cells in Children With Allergen-Induced Phenotype of Bronchial Asthma Treated With Glucoseminylmuramildipeptide

Characteristics of Immunocompetent Cells in Children With Allergen-Induced Phenotype of Bronchial Asthma Treated With Glucoseminylmuramildipeptide

The Mechanisms of the Regulation of Immune Response in Patients with Comorbidity of Chronic Obstructive Pulmonary Disease and Asthma

CLINICAL AND IMMUNOLOGICAL COMPARISONS IN Th- DEPENDENT IMMUNE RESPONSE MECHANISMS AMONG PATIENTS WITH CHRONIC OBSTRUCTIVE PULMONARY DISEASE

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