T cells and IL-17 in lupus nephritis

Koga, Tomohiro; Ichinose, Kunihiro; Tsokos, George C.

doi:10.1016/j.clim.2016.04.010

Cited by 104 publications

(69 citation statements)

References 83 publications

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“…A similar delay in the activation markers were observed in PMA/ionomycin-stimulated TCRβ+CD138+ cells (Supplemental Figure 3A). In SLE, activated T cells participate in the inflammatory process through the production of cytokines such as IFNγ, TNFα and IL-17 [2–4, 7, 31, 32]. We found that after TCR engagement and PMA/ionomycin stimulation, more than 90% of TCRβ+CD138− cells were positive for IFNγ, while less than 30% of TCRβ+CD138+ cells produced IFNγ (Figure 3D, Supplemental Figure 3B and C).…”

Section: Resultsmentioning

confidence: 99%

Disease stage-specific pathogenicity of CD138 (syndecan 1)-expressing T cells in systemic lupus erythematosus

Liu¹,

Takeda

Akkoyunlu³

2020

Preprint

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ObjectiveTo identify and characterize CD138 (syndecan 1)-expressing T cells in SLE-prone mice. MethodsWe characterized CD138-expressing T cells in MRL/Lpr mice by flow cytometry assay and by gene analysis. Functional properties of TCRβ+CD138+ cells were assessed either by activating through TCR or by co-incubating with purified B cells in the presence of auto-antigens. Purified TCRβ+CD138+ cells were adoptively transferred into MRL/Lpr mice and lupus disease was assessed by measuring serum auto-antibodies, proteinuria and by histopathological evaluation of kidney. ResultsWe found that the frequency of TCRβ+CD138+ cells was significantly higher in MRL/Lpr mice than in wild-type MRL mice (p < 0.01), and the increase in their numbers correlated with disease severity. Majority of the TCRβ+CD138+ cells were CD4 and CD8 double-negative and 20% were CD4. Compared to TCRβ+CD138-cells, TCRβ+CD138+ cells exhibited central memory phenotype with reduced ability to proliferate, and produce the cytokines IFNγ and IL-17. When co-cultured with B cells, the ability of TCRβ+CD138+ cells to promote plasma cell formation and autoreactive antibody production was dependent on the presence of auto-antigens and CD4 co-receptor expression. Surprisingly, adoptively transferred TCRβ+CD138+ cells slowed down the disease progression in young MRL/Lpr mice but had the opposite effect when DNA was co-

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Section: Resultsmentioning

confidence: 99%

Disease stage-specific pathogenicity of CD138 (syndecan 1)-expressing T cells in systemic lupus erythematosus

Liu¹,

Takeda

Akkoyunlu³

2020

Preprint

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“…Glomerulonephritis in lupus occurred as a result of complex immune deposit. 32,33 It is possible for the immune complex to split and then eliminated from glomerulus, so the glomerulus function repaired. In this research, all doses tested result in good outcomes to repair the kidney structure and function (Figure 2 and Table 1).…”

Section: Discussionmentioning

confidence: 99%

Repairing Effects of Aqueous Extract of Kalanchoe pinnata (Lmk) Pers. on Lupus Nephritis Mice

Indriyanti¹,

Garmana²,

Setiawan³

2018

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Kalanchoe pinnata (Lmk) Pers (KP) has an immunosuppressive effect on delayed-type hypersensitivity test. Based on it, this research aimed to determine the repairing effects of aqueous extract of KP on lupus nephritis mice and identified its active compound. The KP extract profile was determined using UPLC-QTOF-MS/MS instrument. We examined six mice groups consisting of three curative treatment groups, one standard group receiving prednisone, one preventive group receiving KP extract, and one healthy (healthy and untreated) group. At the end of the experiment, we measured the proteinuria and renal histology parameters. To recognize the active compound in the KP profile, we performed in silico assays for the flavonoid compounds to bind to the glucocorticoid receptor. We played in silico tests for the flavonoid compounds to identify the active compound in the KP profile. We found the repairing effect of KP was detected in the kidney, demonstrated by its low proteinuria level and its better tissue structure. In the curative group, the urine protein level and its glomerular inflammation decreased. In the preventive group, the aqueous extract of KP could prevent lupus nephritis manifestations in the kidney. Bryophyllin A is the most active compound of the KP. However, further research is needed to understand the mechanism involved. We conclude, the aqueous extract, especially its bryophyllin A, have beneficial effects in repairing the function and tissue structure of lupus manifestations in mice kidney.

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“…It has been proposed that Th17 plays crucial roles in the pathogenesis of proinflammatory disorders including autoimmune diseases via the production of IL-17A [17, 18]. IL-21 and IL-23 are important cytokines which play pivotal roles in development and maintenance of Th17, respectively [19].…”

Section: Discussionmentioning

confidence: 99%

Increased Serum Levels of IL-17A and IL-23 Are Associated with Decreased Vitamin D3 and Increased Pain in Osteoarthritis

et al. 2016

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IntroductionOsteoarthritis (OA) is the most common type of arthritis and proinflammatory cytokines have been considered as the main etiologic factor in the pathogenesis of the disease. Serum levels of cytokines, that are associated with innate immunity and TH1 cells, have been analyzed in OA patients, however, there is limited research that profiles cytokines associated with Th17 cells and their relation to vitamin D3 and pain.Material and methodsThe sera from 131 patients with OA and 262 healthy controls were evaluated for serum levels of IL-17A, IL-21, IL-23 and vitamin D3 using ELISA.ResultsSerum levels of IL-17A, and IL-23 were statistically higher in OA patients than in healthy controls, while IL-21 and vitamin D3 were significantly lower in OA patients when compared to controls. A significant positive correlation was found between the serum levels of IL-17A and IL-23 using WOMAC pain scores and vitamin D3 serum levels.DiscussionThe results suggest that IL-17A plays a significant role in OA pathogenesis and the induction of pain. Decreased serum levels of vitamin D3 may reflect a positive role played by the factor in the regulation of immune responses in OA patients.

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T cells and IL-17 in lupus nephritis

Cited by 104 publications

References 83 publications

Disease stage-specific pathogenicity of CD138 (syndecan 1)-expressing T cells in systemic lupus erythematosus

Disease stage-specific pathogenicity of CD138 (syndecan 1)-expressing T cells in systemic lupus erythematosus

Repairing Effects of Aqueous Extract of Kalanchoe pinnata (Lmk) Pers. on Lupus Nephritis Mice

Increased Serum Levels of IL-17A and IL-23 Are Associated with Decreased Vitamin D3 and Increased Pain in Osteoarthritis

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