Synthesis of curcumin loaded polymeric nanoparticles from crab shell derived chitosan for drug delivery

Samrot, Antony V.; Burman, Ujjala; Philip, Sheryl Ann; Shobana, Nagarajan; Chandrasekaran, Kumar

doi:10.1016/j.imu.2017.12.010

Cited by 90 publications

(42 citation statements)

References 41 publications

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“…In a study by Samrot et al, curcumin-loaded CsNPs were synthesized from crab shell for drug delivery using two chelators (TPP and BaCl 2 ) in preparation of CsNPs. 37 They observed an initial drug burst from the biopolymer that then become steady, and the CsNPs chelated with BaCl 2 led to sustained drug release for more than 70 minutes. Regarding CsNPs chelated with TPP, the samples had a stable drug release profile; drug release increased from the 20 th to the 60 th minute and then gradually decreased until the 100th minute, followed by a stable release.…”

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines

Loutfy¹,

Elberry²,

Farroh³

et al. 2020

IJN

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Purpose: Current direct-acting antiviral agents for treatment of hepatitis C virus genotype 4a (HCV-4a) have been reported to cause adverse effects, and therefore less toxic antivirals are needed. This study investigated the role of curcumin chitosan (CuCs) nanocomposite as a potential anti-HCV-4a agent in human hepatoma cells Huh7. Methods: Docking of curcumin and CuCs nanocomposite and binding energy calculations were carried out. Chitosan nanoparticles (CsNPs) and CuCs nanocomposite were prepared with an ionic gelation method and characterized with TEM, zeta size and potential, and HPLC to calculate encapsulation efficiency. Cytotoxicity studies were performed on Huh7 cells using MTT assay and confirmed with cellular and molecular assays. Anti-HCV-4a activity was determined using real-time PCR and Western blot. Results: The strength of binding interactions between protein ligand complexes gave scores with NS3 protease, NS5A polymerase, and NS5B polymerase of -124.91, -159.02, and -129.16, for curcumin respectively, and -68.51, -54.52, and -157.63 for CuCs nanocomposite, respectively. CuCs nanocomposite was prepared at sizes 29-39.5 nm and charges of 33 mV. HPLC detected 4% of curcumin encapsulated into CsNPs. IC50 was 8 µg/mL for curcumin and 25 µg/ mL for the nanocomposite on Huh7 but was 25.8 µg/mL and 34 µg/mL on WISH cells. CsNPs had no cytotoxic effect on tested cell lines. Apoptotic genes' expression revealed the caspasedependent pathway mechanism. CsNPs and CuCs nanocomposite demonstrated 100% inhibition of viral entry and replication, which was confirmed with HCV core protein expression. Conclusion: CuCs nanocomposite inhibited HCV-4a entry and replication compared to curcumin alone, suggesting its potential role as an effective therapeutic agent. Plain Language SummaryHepatitis C virus genotype 4a (HCV-4a) is a major public health problem, especially among the Egyptian population. There are more than 70 million individuals infected worldwide. It is the leading cause of chronic liver diseases, cirrhosis, and hepatocellular carcinoma. Newly emerged anti-HCV viral agents have recently become available on the market but with serious complications, high cost, and possible development of resistance. This has encouraged scientists to search for alternative, safer antiviral approaches. Nanoparticles offer unique physical properties that have associated benefits for antimicrobial activity or as drug carrier that can improve antiviral therapy. The significance of our research is in establishment of a natural nanoparticle drug carrier system that we screened with computer simulation studies and in cells against HCV-4a replication into human hepatoblastoma cell

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Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines

Loutfy¹,

Elberry²,

Farroh³

et al. 2020

IJN

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“…231 Pure Biopolymer Nanoparticles (PBN) Chitosan, a cationic biocompatible and biodegradable linear polysaccharide, containing d-glucosamine and N-acetyl glucosamine units, which is extracted from the exoskeleton of crustacean arthropods such as insects, crabs, lobster and shrimps 232 reported to be the best example of natural pure biopolymer to deliver plant components such as curcumin, quercetin, 233 and transresveratrol with better mucoadhesion, solubility, dissolution rate, and specific targeting. 234,235 Additionally, chitosan has been utilized for various biomedical applications such as in tissue engineering in the form of scaffolds, drug delivery carriers, fabricating surgical thread, bone healing, and as a wound dressing substance. On the other hand, modified chitosan molecules such as dextran sulfate-conjugated chitosan, biotinylated and galactosylated chitosan are well developed with advanced properties such as altering the surface charge, providing pH-sensitive swelling, more stability, enhancing bioactivity by modifying targeting to the specific site of action ( Figure 16).…”

Section: Micellementioning

confidence: 99%

Novel Drug Delivery Systems for Loading of Natural Plant Extracts and Their Biomedical Applications

Rahman

Othman

Hammadi

et al. 2020

IJN

144

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Many types of research have distinctly addressed the efficacy of natural plant metabolites used for human consumption both in cell culture and preclinical animal model systems. However, these in vitro and in vivo effects have not been able to be translated for clinical use because of several factors such as inefficient systemic delivery and bioavailability of promising agents that significantly contribute to this disconnection. Over the past decades, extraordinary advances have been made successfully on the development of novel drug delivery systems for encapsulation of plant active metabolites including organic, inorganic and hybrid nanoparticles. The advanced formulas are confirmed to have extraordinary benefits over conventional and previously used systems in the manner of solubility, bioavailability, toxicity, pharmacological activity, stability, distribution, sustained delivery, and both physical and chemical degradation. The current review highlights the development of novel nanocarrier for plant active compounds, their method of preparation, type of active ingredients, and their biomedical applications.

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“…Samrat et al . 6 The demineralized extract of chitosan obtained from Metacarcinus magister shells was used. The curcumin loaded nanoparticles was prepared using extracted chitosan and sodium tripolyphosphate and barium chloride as chelators.…”

Section: Antony Vmentioning

confidence: 99%

Therapeutic Review on Nanocurcumin

2020

CHCMJ

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Synthesis of curcumin loaded polymeric nanoparticles from crab shell derived chitosan for drug delivery

Cited by 90 publications

References 41 publications

<p>Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines</p>

<p>Antiviral Activity of Chitosan Nanoparticles Encapsulating Curcumin Against Hepatitis C Virus Genotype 4a in Human Hepatoma Cell Lines</p>

<p>Novel Drug Delivery Systems for Loading of Natural Plant Extracts and Their Biomedical Applications</p>

Therapeutic Review on Nanocurcumin

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