Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

Brown, Rachel; Smith, W. Ewen; Graham, Duncan

doi:10.1016/s0040-4039(01)00108-3

Cited by 11 publications

(6 citation statements)

References 14 publications

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“…In addition, there have been a large number of dyes custom synthesised for use as SERRS labels with DNA. [41][42][43][44] Basic strategy for detection As stated above, there are a number of different labels that can be used to generate a SERRS active piece of DNA. The exact nature of the label dictates the methodology used to obtain SERRS for the labelled piece of DNA.…”

Section: Intercalatorsmentioning

confidence: 99%

DNA detection by surface enhanced resonance Raman scattering (SERRS)

Faulds

Smith

Graham

2005

Analyst

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This Education article outlines the different ways in which surface enhanced resonance Raman scattering (SERRS) can be used for the detection of DNA. The use of various different SERRS detection strategies that have allowed both sensitive and selective detection to be obtained is covered. Detection of DNA by SERRS involves the use of a dye with the DNA, whether as an intercalator or by direct covalent attachment. This generates strong SERRS signals that indicate the presence of the specific DNA sequence. The SERRS detection of DNA in different molecular biological assays is also discussed.

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Section: Intercalatorsmentioning

confidence: 99%

DNA detection by surface enhanced resonance Raman scattering (SERRS)

Faulds

Smith

Graham

2005

Analyst

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“…12,18 The physico-chemical characteristics of the silver sol obtained were determined by UV-VIS spectroscopy, 19 transmission electron spectroscopy as well as photon correlation spectroscopy 20 and were in accord with those reported in the scientific literature. For SERRS analysis of indigo the silver sol was aggregated using 35 mL of 1 mol dm 23 HCl. However, in order to analyse both synthesized and reference samples of indigo carmine, aggregation of the silver sol was achieved by addition of 150 mL of a 0.01% aqueous solution of poly(l-lysine).…”

Section: In a Novel Application Serrs Has Been Used By Employing A Si...mentioning

confidence: 99%

Analysis of the conversion of indigo into indigo carmine dye using SERRS

et al. 2004

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“…In the simplest approach, we took benzotriazole and synthesised a phosphoramidite [1] which could then be used in routine solid phase synthesis of oligonucleotides, at the 59-end, to provide a method of surface attachment to silver nanoparticles. 44 Although simple in chemical terms, benzotriazole does not contain a coloured group and as such does not provide a resonance contribution and lacks the same sensitivity of a chromophoric system. Therefore the next step was to make a benzotriazole azo dye phosphoramadite [2].…”

Section: Benzotriazolementioning

confidence: 99%