Biosensing using silver nanoparticles and surface enhanced resonance Raman scattering

Graham, Duncan; Faulds, Karen; Smith, W. Ewen

doi:10.1039/b607904k

Cited by 117 publications

(63 citation statements)

References 60 publications

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“…To further confirm the AgNP synthesis mechanism via the Ag 2 O route, AgNPs were synthesized with 2 mmol/L AgNO 3 and 0.4 wt% AL ls at 85 • C via two procedures: (1) direct mixing of AgNO 3 and AL ls at pH 10.01 for 60 min; (2) mixing AgNO 3 with neutral AL ls solution for 30 min then adding NaOH for another 60 min. The TEMs showed AgNPs obtained via direct mixing at pH 10.01 ranged from 3 to 40 nm in sizes with an average feret diameter of 9.9 (±3.8) nm (Fig.…”

Section: Reaction Mechanismmentioning

confidence: 99%

“…Silver nanoparticles have drawn much attention in research due to their many desirable properties including high electrical conductivity [23], excellent catalytic activity in organic redox reactions [16,24], surface plasmon resonance [3,10] and anti-microbial properties [7,14]. Synthesis of silver nanoparticles typically involves "bottom-up" reduction of silver salts, most commonly silver nitrate, often in the presence of capping agents to fine-tune particle sizes and shapes [11,12,[19][20][21]25,26].…”

Section: Introductionmentioning

confidence: 99%

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Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Hsieh

2016

International Journal of Biological Macromolecules

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a b s t r a c tSilver nanoparticles (AgNPs) were robustly synthesized from aqueous AgNO 3 with alkali lignin (low sulfonate) (AL ls ) severing as dual reducing and capping agent. The AgNP synthesis mechanisms were highly pH dependent. Under neutral and acidic conditions, polydispersed AgNPs were synthesized via the self-catalyzed reduction of Ag + on instantaneously formed Ag 2 O surfaces followed by the slower pseudo-first order reduction. The Ag 2 O nanoparticles functioned as the nucleating sites for the reduction of remaining silver cations to form AgNPs whose size and size distribution strongly dependent of lignin concentrations. AgNPs were optimally synthesized by reducing 2 mmol/L AgNO 3 with 0.16 wt% AL ls at pH 10 and 85• C in 30 min to near 100% yield in bimodal distributed sizes with 23% and 77% in feret diameters of 7.3 (±2.2) nm and 14.3 (±1.8) nm, respectively.

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Section: Reaction Mechanismmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Hsieh

2016

International Journal of Biological Macromolecules

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“…[1][2][3] The advantages of SERS as a labeling/read-out approach include its reduced susceptibility to photobleaching, narrow vibrational Raman bands for spectral multiplexing, and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Wang

Salehi

Schütz

et al. 2013

Analyst

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Rationally designed gold/silver nanoshells (Au/Ag-NS) with plasmon resonances optimized for red laser excitation in order to minimize autofluorescence from clinical samples exhibit scattering cross-sections, which are ca. one order of magnitude larger compared with solid quasi-spherical gold nanoparticles (Au-NPs) of the same size. Hydrophilic stabilization and sterical accessibility for subsequent bioconjugation of Au/Ag-NS is achieved by coating their surface with a self-assembled monolayer (SAM) of rationally designed Raman reporter molecules comprising terminal mono-and tri-ethylene glycol (EG) spacers, respectively. The stability of the hydrophilically stabilized metal colloid was tested under different conditions. In contrast to metal colloids coated with a SAM without terminal EG spacers, the hydrophilically stabilized SERS particles do not aggregate under physiologically relevant conditions, i.e., buffer solutions with high ionic strength. Using these rationally designed SERS particles in conjunction with a microspectroscopic acquisition scheme, a sandwich immunoassay for the sensitive detection of interleukin-6 (IL-6) was developed. Several control experiments demonstrate the high specificity of the assay towards IL-6, with a lowest detectable concentration of ca. 1 pg mL À1 . The signal strength of the Au/Ag-NS is at least one order of magnitude higher compared with hydrophilically stabilized, non-aggregated solid quasi-spherical Au-NPs of the same size.

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“…This makes it ideal for the study of biomolecules. Thus SERRS has been used to study heme proteins, 9 to study the mechanism of electron transfer in cytochromes, 10 and more recently for trace detection of DNA 11,12 and enzyme activity. 13 The sensitivity of SERRS exceeds that of fluorescence, which is currently the most prolific technique employed for studying biomolecules, as proven theoretically 14 and experimentally.…”

Section: Introductionmentioning

confidence: 99%

Reproducible SERRS from structured gold surfaces

Mahajan

Baumberg

Russell

et al. 2007

Phys. Chem. Chem. Phys.

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Metallic substrates with ordered spherical cavities have been shown to be very effective for surface-enhanced Raman scattering (SERS) and can be fabricated reproducibly using electrodeposition. The sensitivity of detection is increased by several orders of magnitude by using surface-enhanced resonance Raman scattering (SERRS). In this report we demonstrate SERRS for the first time on electrodeposited gold films templated with colloidal spheres and demonstrate the reproducibility of the response. We also obtain a direct comparison between SERRS and SERS by choosing two dyes, Cy5t and Cy3t, which are similar in structure but differ in their excitation maxima, such that one is resonant and the other non-resonant with our laser excitation. As expected, the resonant enhancement is found to be of the order of 10 3 over and above that for SERS. The net SERRS enhancements are shown to be of the order of 10 9 . We also find that the resonant enhancement profile of the different peaks for the chromophore follows the plasmonic resonance absorption spectrum obtained for the structured surface.

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Biosensing using silver nanoparticles and surface enhanced resonance Raman scattering

Abstract: Silver nanoparticles can be used to provide excellent surface enhanced resonance Raman scattering. Control of the surface chemistry and the use of appropriate protocols enables effective sensing of biomolecules.

Cited by 117 publications

References 60 publications

Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Silver nanoparticle synthesis using lignin as reducing and capping agents: A kinetic and mechanistic study

Microspectroscopic SERS detection of interleukin-6 with rationally designed gold/silver nanoshells

Reproducible SERRS from structured gold surfaces

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