Synthesis, Characterization and In vitro Antimicrobial Activity of Florfenicol-Chitosan Nanocomposite

Abstract

In this paper, rapid, easy and cheap sonochemical method was described for synthesis of florfenicol-chitosan nanocomposite and to evaluate its anti-bacterial effect against Escherichia coli (ATCC35218), Salmonella typhymurium (ATCC14028) and Staph. aureus (ATCC29213). Florfenicol-chitosan nanocomposite was fully characterized for index, identification and morphology properties. Results of zeta potential for florfenicol-chitosan nanocomposite was -28 mV. Brunner-Emmett-Teller theory (BET) surface area was found to be 13.3, 73.2 and 103.69 m 2 /g for florfenicol, chitosan nanoparticles and florfenicol-chitosan nanocomposite, respectively. Raman charts confirmed the formation of florfenicol-chitosan nanocomposite without any contamination. Transmission electron microscope (TEM), scanning electron microscope (SEM) and atomic force microscope (AFM) images and data illustrated spherical to sub spherical shape of florfenicol nanoparticles on sheet shape of chitosan with size less than 75 nm. Remarkable results of florfenicol-chitosan nanocomposite as anti-bacterial agent illustrated the power of nanotechnology. However, antimicrobial activity was screened where the zone of inhibitions caused by the prepared nanocomposite were 24.7 mm, 30.6 mm and 29.3 mm compared to 17.7 mm, 16 mm and 18.7 mm of the native drug against E. coli, Salmonella typhymurium and Staphylococcus aureus, respectively.

Upgrading the preparation of high-quality chitosan from Procambarus clarkii wastes over the traditional isolation of shrimp chitosan

“…The result of FTIR of P. clarkii chitosan revealed different functional groups of organic compounds such as out-of-plane bending (564 cm −1 ), C—O—C stretching (711 cm −1 ), and CH2 stretching (1174 cm −1 ) in chitosan. The most characteristic Raman shift peaks of the P. clarkii chitosan sheet are located between 2750 and 2930 cm −1 and related to (C-H), (CH2), and (CH3), confirming its identity in accordance with ( Varma and Vasudevan, 2020 , Youssef et al, 2021 ). On the other hand, Fernández-Pan et al (2010) reported two commercial chitosan samples from cold-water shrimp shells having high degrees of deacetylation (96 and 100%).…”

“…The 532 nm He-Cd edge laser line with grating 1800 (450–850 nm). ND filter 3.2% was used to avoid sample burning employing 10 sec acquisition time, 5 accumulations without delay time, and spike filter ( Youssef et al, 2021 ).…”

Evaluation of the wound healing effect of neomycin-silver nano-composite gel in rats

“…Nanoparticles have physicochemical characteristics superior to bulk materials due to their enormous surface to volume proportion, higher reactivity, stability, bioactivity, bioavailability, controlled particle size, controlled release of loaded drugs, and site-specific targeting. 5 – 8 …”

Synthesis, spectroscopic characterization, density functional, thermal studies, and molecular docking simulation of rare earth element complexes

“…This can be attributed to the involvement of the υ(C═N) in coordination to the Ln(III) ions. [22,23,26,[29][30][31] The bands appeared at 1622-1652 cm À1 were attributed to the carbonyl v(C═O) group. In the spectra of all the complexes, the group of carbonyl v(C═O) was significantly moved to a lower frequency due to hydrogen bond formation with OH b group.…”

“…In continuation to our previous work, [ 22,23 ] the complexity behavior of several ligand substitutions with lanthanum(III) metal ions (La(III), Er(III), and Yb(III)) was investigated, and the resulting complexes were characterized with different analytical methods as well as physicochemical methods. In addition, certain complexes also undergo optoelectronic properties including an estimate of their optical band distance and energy for the highest occupied (HOMO) and the lowest unoccupied (LUMO) molecular orbitals to explore its potential for use with optoelectronic devices.…”