Evaluation of the wound healing effect of neomycin-silver nano-composite gel in rats

Abstract

Objectives: Both nano silver and neomycin have wound healing properties. Silver nanoparticles have been used as main compounds for therapeutic drug delivery systems against various ailments. The present study aimed to prepare a neomycin silver nano-composite gel easily, rapidly, and cheaply method to improve wound healing. Methods: Forty-five Wistar rats (150–200 g) divided into nine groups: wound untreated, wound fusidic acid treated, wound neomycin treated, three groups with wound and neomycin silver nano-composite gel at 1:1, 1:2, and 1:3 concentrations, respectively, and three groups wound treated silver nano gel at the previous concentrations, respectively. Percentages of wound healing and histopathological examination of the wound area were assessed in all groups. Results: Atomic force microscopy (AFM) and transmission electron microscopy (TEM) images demonstrated the spherical shape of neomycin silver nano-composite gel without aggregation but homogenous dispersion in a gel matrix. Dynamic light scattering (DLS) showed a 4 nm size of nano silver, which agrees with AFM image data analysis but not with TEM image due to the good coating of the gel matrix to silver nanoparticles. Dynamic light scattering Zeta potential was −21 mV, illustrating the high bioactivity of the neomycin silver nano-composite. The groups receiving neomycin silver nano-composite gel showed a significantly higher and dose dependent wound healing compared to other treatment groups. Conclusion: The present work confirmed the potential wound healing activity of neomycin silver nano-composite gel compared to either alone.

Beneficial Effect of Wound Dressings Containing Silver and Silver Nanoparticles in Wound Healing—From Experimental Studies to Clinical Practice

“…Authors believe that for this reason in the nearest future, most severe infected wounds will be treated with combined therapy of AgNPs and standard antibiotics. The effectiveness of such therapies has been demonstrated in the studies that explored dressings combining silver with colistin or neomycin [ 68 , 69 ]. Several natura or synthetic biomaterials containing silver nanoparticles have already presented promising results in pre-clinical and clinical trials.…”

“…To reduce the side effects of silver and other antimicrobial substances, scientists are trying to combine them with substances that have a synergistic effect. Banna et al [ 68 ] presented results of the study in which a gel containing both neomycin and AgNPs was tested in the treatment of wounds in rats. 45 rats were divided in such a way that some of the animals were treated with a gel containing only Ag+, some only with neomycin, and some with a dressing containing both neomycin and AgNPs.…”

Green Analytical Chemistry to Eco-Friendly HPLC Techniques in Pharmaceutical Analysis: A Review

“…Purified water was employed as the mobile phase in a mixed-mode polar embedded column for the separation of polar compounds (nucleosides, nucleic bases, and purine alkaloids). The long alkyl chains that make up this stationary phase's reverse phase and the ion-exchange functional groups connected to the chain's terminal end are what give this column its ion-exchange and reversed phase properties [34]. This type of stationary phase could expand the options for component separation with various polarities, making WRP-LC a more appealing strategy for the development of green chromatographic methods.…”

Therapeutic and Toxicological Aspects of Some Metal Nanoparticles on The Central Nervous System: A Review

“…To utilize these unique qualities, nanotechnology requires developing and using tailored materials at the nanoscale. Humans have been faced with a series of nanoparticles (Nanoparticles) emanating from numerous activities like biomedical, welding and combustion applications [6].…”