Synthesis and modification of supports with an alkylamine and their use in albumin adsorption

Abstract

The morphological effect of polymeric networks (R) modified with terminal amino groups was studied on the adsorption of bovine serum albumin (BSA). Networks of ethylene glycol dimethacrylate and 2‐hydroxyethyl methacrylate [poly (EGDMA‐co‐HEMA)] were synthesized by suspension polymerization, using different EGDMA contents and agitation speeds. These matrices were characterized by FTIR, mercury intrusion porosimetry, SEM, and swelling degree. The increase of the EGDMA concentration led to the formation of networks with the highest crosslinking degree and porosity. An earlier phase separation yielded a higher aggregation of rigid microspheres, also forming stable pore systems. The increase in coalescence frequency, together with the impeller speed, and the decrease of the stabilizer molecules led to an increment in drop size. Large fused aggregates of microspheres were formed with additional loss of small pores as the stirring was increased, attaining also a higher pore volume (Vp) and a slight decrease of the surface area. Once characterized, networks were activated with butanediolglycidyl ether (BDGE), and then reacted with hexamethylenediamine (HMDA) through coupling reaction. Only the R‐BDGE‐HMDA networks synthesized with the highest EGDMA content and agitation speed showed BSA adsorption. Their base matrices exhibited a Vp higher than 1.4 mL/g, which allows easier protein diffusion into the support. © 2008 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 46: 2557–2566, 2008

Study of amino ligands fixation to macroporous supports and their influence on albumin adsorption

“…The activation reaction was first carried out using a mol ratio 1:12:0.5 of hydroxyl groups, oxirane and base, respectively. Figure 3 shows a typical U‐shape activation curve, where the Ech derivatives and BDGE present a similar behavior related to two opposed effects described in a previous work by Gomez and Strumia 20. One effect is characterized by the amount of hydroxyl groups present in the matrix; the other relates to the diffusion into the network together with its accessible surface.…”

“…In all cases, the polymerization yield was between a 95 and 98 % w/w. In a previous work20 these networks have just been characterized, where the increase of the EGDMA concentration was found to lead to an earlier phase separation during polymerization. Therefore, networks with a higher crosslinking degree (∼1/ Q v ), S s value and porosity (Table 1) were attained.…”

“…Since the base supports of the epoxy‐activated derivatives III and IV display (Table 1) a higher crosslinking density (∼1/ Q v ) and porosity, the largest extension of the coupling reaction was carried out on the accessible oxirane groups of the support surface. These pendant oxirane groups able to react were mostly attached to the network surface between pores equal to or larger than the reagent from which the former were generated (Scheme ) 20. Therefore, the space disposition of the pendant oxirane groups and the accessibility of the alkyldiamine into the support have controlled the performance of the coupling reaction.…”

“…On the other hand, the synthesis of macroporous networks with interconnected pores that allow the diffusion of large solutes and that provide large surface area for further chemical modifications is a matter of discussion in several research groups 14–17. In previous works, we have analyzed the construction of matrices through suspension polymerization, examining several reaction conditions such as type of monomers, diluents, crosslinker content, and stirring speed in the development of the porous structure 18–20. Therefore, in this research amino ligands were attached to the network from the construction of different spacer arms, and the performance of BSA adsorption on the amino ligands obtained was then analyzed.…”

Selective separation of human serum albumin with copper(II) chelated poly(hydroxyethyl methacrylate) based nanoparticles

“…Ligand stability is becoming an increasingly important consideration. The recent trend, therefore, has been to replace high molecular mass biological ligands with small molecular-mass pseudospecific ligands [9][10][11].…”