Selective separation of human serum albumin with copper(II) chelated poly(hydroxyethyl methacrylate) based nanoparticles

Karakoç, Veyis; Yılmaz, Erkut; Türkmen, Deniz; Öztürk, Nevra; Akgöl, Sinan; Deni̇zli̇, Adil

doi:10.1016/j.ijbiomac.2009.04.023

Cited by 34 publications

(10 citation statements)

References 21 publications

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“…Functional polymers based on hydroxyethyl methacrylate (HEMA) monomer are an interesting class of materials because of their excellent biomedical properties such as biocompatibility [51][52][53][54][55][56]. In this article, the performance of a polyHEMA-based membrane carrying protein A for the purification of IgG has been investigated in detail.…”

Section: Resultsmentioning

confidence: 99%

Performance of Protein-A-Based Affinity Membranes for Antibody Purification

Uzun

Türkmen

Karakoç

et al. 2011

Journal of Biomaterials Science, Polymer Edition

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The preparation of affinity membranes for application in antibody purification studies is described here. Protein-A-attached poly(hydroxyethyl methacrylate-N-methacryloyl-L-alanine) (PHEMAAL) membranes were produced by a photopolymerization technique and then characterized by swelling tests, surface area measurements, contact angle and scanning electron microscopy (SEM) studies. The water swelling ratio of the PHEMAAL membrane was 133.2%. PHEMAAL membranes have large pores with a size in the range of 5-10 μm. Protein A was covalently attached onto the PHEMAAL membranes via cyanogen bromide (CNBr) activation. Maximum protein A loading was 4.7 mg/g. There was a very low non-specific IgG adsorption onto the PHEMAAL membranes, about 0.38 mg/g. The maximum IgG adsorption on the PHEMAAL-protein A membrane was found to be 9.8 mg/g at pH 7.4 from aqueous solutions. Higher adsorption amount was observed from human plasma (up to 37.3 mg/g). Adsorbed IgG was eluted using 0.1 M glycine-HCl buffer (pH 3.5) with a purity of 93%. PHEMAAL-protein A membrane was used for repetitive adsorption/elution of IgG without noticeable loss in IgG adsorption amount after 10 cycles. The PHEMAAL-protein A membrane showed several advantages, such as simpler preparation procedure, good selectivity for IgG purification from human plasma and good stability throughout repeated adsorption-elution cycles.

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Section: Resultsmentioning

confidence: 99%

Performance of Protein-A-Based Affinity Membranes for Antibody Purification

Uzun

Türkmen

Karakoç

et al. 2011

Journal of Biomaterials Science, Polymer Edition

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“…Pseudospecific adsorbents may hold certain advantages for industrial affinity separations since the ligands are not likely to cause an immune response in case of leakage into the product. [64][65][66][67][68] An expensive step in the preparation process of metal-chelating adsorbent is coupling of a chelating ligand to the adsorption matrix. In this article, MAH is used as the metal-chelating ligand, and there is no need to activate the matrix for the chelating-ligand immobilization.…”

Section: Discussionmentioning

confidence: 99%

Antibody purification from human plasma by metal‐chelated affinity membranes

Yavuz

Bereli

Armutçu

et al. 2011

J of Applied Polymer Sci

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The aim of this study is to investigate in detail the feasibility of poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo-polymerization technique. Then, Zn 2þ , Ni 2þ , Co 2þ , and Cu 2þ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 lmol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL).Further increase was observed with the metal-chelated PHEMAH membranes (up to 118 mg/mL). The metal-chelate affinity membranes allowed the one-step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu 2þ > Ni 2þ > Zn 2þ > Co 2þ . Adsorbed IgG was eluted using 250 mM EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal-chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity.

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“…lnQ e = lnK f + 1/n lnC e (4) where Q e is the adsorption capacity (mg/g), C e is the concentration of biomolecule in solution (mg/L), K f and n being The constants for the isotherms are reported in Table 3. The Langmuir isotherm having a higher correlation coefficient was in better aggrement with experimental results.…”

Section: Adsorption Isotherms and Kineticsmentioning

confidence: 99%

“…Their surface can be easily modified via organic (polymer) or inorganic (metal or oxide) surfaces. Magnetic nanoparticles containing polymers could be eassily separated from medium using magnetic separation technique without centrifugation, precipitation, and filtration, thereby offering several advantages in terms of processing time and economic cost (4)(5).…”

Section: Introductionmentioning

confidence: 99%

Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography

Üzek

Özkara

Güngüneş

et al. 2014

Separation Science and Technology

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Magnetic poly(2-hydroxyethyl methacrylate-co-N-methacryloyl-L-phenyl alanine), p(HEMA-co-MAPA) nanoparticles were produced by emulsion polymerization technique. The nanoparticles were characterized by zetasizer, atomic force microscopy (AFM), transmission electron microscopy (TEM), electron spin resonance spectroscopy (ESR), and Fourier transformed infrared spectroscopy (FTIR) techniques. Experimental conditions were optimized for DNA adsorption in aqueous media. The effects of several factors (i.e., temperature, pH, equilibrium concentration of DNA, salt type, and ionic strength) on adsorption capacity were examined in batch studies. The maximum DNA adsorption capacity was 211.3 mg/g for nanoparticles consisting of magnetite. The adsorption isotherms and kinetics were also examined. The Langmuir model and pseudo second-order kinetics are the best fitted to the data. Following the ten adsorption-desorption cycles, the decrease in adsorption capacity was only 12%. Finally, plasmid DNA was purified from E.coli lysate by magnetite nanoparticles consisting of MAPA. The purity and molecular mass of purified plasmid DNA were determined by the use of agarose gel electrophoresis resulting in 7000 base pairs for molecular mass and OC pDNA form following elution.

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Selective separation of human serum albumin with copper(II) chelated poly(hydroxyethyl methacrylate) based nanoparticles

Cited by 34 publications

References 21 publications

Performance of Protein-A-Based Affinity Membranes for Antibody Purification

Performance of Protein-A-Based Affinity Membranes for Antibody Purification

Antibody purification from human plasma by metal‐chelated affinity membranes

Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography

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