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/g, with a swelling ratio of 60.2% after the template was removed from the monolith by 0.1M EDTA solution. The maximum adsorption capacity of PHEMACFe 3þ monolith for Fe 3þ ion was 0.76 mg/g. The adsorption behavior of the monolith has been successfully described by the Langmuir isotherm. It was determined that the relative selectivity of PHEMAC-Fe 3þ monolith was 59.7 and 37.0 times greater than that of the nonimprinted PHEMAC monolith as compared with the Cd 2þ and Ni 2þ ions, respectively. The PHEMAC-Fe 3þ monolith was recovered and reused many times without any significant decrease in its adsorption capacity.

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A novel magnetic poly(ethylene glycol dimethacrylate-N-methacryloly-L-histidinemethylester) [m-poly(EGDMA-(MAH)] support was prepared for purification of immunoglobulin G (IgG) in a magnetically stabilized fluidized bed by suspension polymerization. Elemental analysis of the magnetic beads for nitrogen was estimated as 70 micromol MAH/g polymer. Magnetic poly(EGDMA-MAH) beads were used in the separation of immunoglobulin-G (IgG) from aqueous solutions and/or human plasma in a magnetically stabilized fluidized bed system. IgG adsorption capacity of the beads decreased with an increase in the flow rate. The maximum IgG adsorption was observed at pH 6.0 for MES buffer. IgG adsorption onto the m-poly(EGDMA) was negligible. Higher adsorption values (up to 262 mg/g) were obtained in which the m-poly(EGDMA-MAH) sorbents were used from aqueous solutions. Higher amounts of IgG were adsorbed from human plasma (up to 320 mg/g) with a purity of 87%. IgG molecules could be repeatedly adsorbed and desorbed with these sorbents without noticeable loss in their IgG adsorption capacity.

The aim of this study is to prepare an ion-imprinted monolith which can be utilized to remove Fe 3+ from beta thalassemia patient plasma. Fe 3+ , as a template, was initially complexed with N-methacryloyl-(L)-cysteine methyl ester (MAC) to form MAC-Fe 3+ (the complex monomer), which was then polymerized with hydroxyethyl methacrylate (HEMA) to constitude a Fe 3+ -imprinted poly(HEMA-MAC) monolith (PHEMAC-Fe 3+ ) by bulk polymerization method. The template (Fe 3+ ) was removed from the polymer by 0.1 M EDTA solution. The specific surface area of PHEMAC-Fe 3+ was found to be 35.2 m 2 /g with a swelling ratio of 60.2%. A maximum adsorption capacity of 150 µg Fe 3+ /g was observed with PHEMAC-Fe 3+ . It was determined that PHEMAC-Fe 3+ possesses relative selectivity coefficients for Fe 3+ /Cd 2+ and Fe 3+ / Ni 2+ , which are 42.6 and 36.1, respectively, times greater than nonimprinted monolith (produced in the absence of Fe 3+ , PHEMAC). The PHEMAC-Fe 3+ monolith has been recovered and reused many times without a significant decrease in its adsorption capacity.

As alternative hydrophobic adsorbent for DNA adsorption, supermacroporous cryogel disks were synthesized via free radical polymerization. In this study, we have prepared two kinds of cryogel disks: (i) poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan) [p(HEMA-MATrp)] cryogel containing specific hydrophobic ligand MATrp; and (ii) monosize p(HEMA-MATrp) particles synthesized via suspension polymerization embedded into p(HEMA) cryogel structure to obtain p(HEMA-MATrp)/p(HEMA) composite cryogel disks. These cryogel disks containing hydrophobic functional group were characterized via swelling studies, Fourier transform infrared spectroscopy, elemental analysis, surface area measurements and scanning electron microscopy. DNA adsorption onto both p(HEMA-MATrp) cryogel and p(HEMA-MATrp)/p(HEMA) composite cryogels was investigated. Maximum adsorption of DNA on p(HEMA-MATrp) cryogel was found to be 15 mg/g polymer. Otherwise, p(HEMA-MATrp)/p(HEMA) composite cryogels significantly increased the DNA adsorption capacity to 38 mg/g polymer. Composite cryogels could be used repeatedly without significant loss on adsorption capacity after 10 repetitive adsorption-desorption cycles.