Evaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC system

Doğan, Ali; Özkara, Serpil; Sarı, Müfrettin Murat; Uzun, Lokman; Deni̇zli̇, Adil

doi:10.1016/j.jchromb.2012.02.036

Cited by 34 publications

(28 citation statements)

References 37 publications

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“…Cryogels are another group of monoliths that have been used in AMC [10, 15, 66–72]. These supports can have good chemical and physical stability and have been reported to be cost-effective to use [66].…”

Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

“…These supports can have good chemical and physical stability and have been reported to be cost-effective to use [66]. Cryogels are gel matrices that are formed in the presence of moderately frozen solutions of monomeric or polymeric precursors.…”

Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

“…Cryogel monoliths can be prepared in the form of either columns or membranes [10]. Like other types of monoliths, cryogels often allow the use of high flow rates, making it possible to pass through large sample volumes in a short amount of time [66, 67]. One possible limitation of a cryogel is its relatively low surface area, which could limit the amount of binding agent that can be immobilized to this support for AMC [30].…”

Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

“…Antibodies, concanavalin A, dyes, and metal-ion chelates have all been coupled to cryogel monoliths and used in applications such as the purification and depletion of proteins and enzymes from samples [66, 68–71]. For instance, a cryogel comprised of HEMA and containing the immobilized dye Cibacron Blue F3GA was utilized for the purification of human interferon (see Figure 7) [66]. Another report used a similar cryogel for the depletion of albumin from human serum [72].…”

Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

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Affinity monolith chromatography: a review of principles and recent analytical applications

et al. 2012

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Affinity monolith chromatography (AMC) is a type of liquid chromatography that uses a monolithic support and a biologically-related binding agent as a stationary phase. AMC is a powerful method for the selective separation, analysis or studies of specific target compounds in a sample. This review discusses the basic principles of AMC and recent developments or applications of this method, with particular emphasis being given to work that has appeared in the last five years. Various materials that have been used to prepare columns for AMC are examined, including organic monoliths, silica monoliths, agarose monoliths and cryogels. These supports have been used in AMC for formats that have ranged from traditional columns to disks, microcolumns and capillaries. Many binding agents have also been employed in AMC, such as antibodies, enzymes, proteins, lectins, immobilized metal-ions and dyes. Some applications that have been reported with these binding agents in AMC are bioaffinity chromatography, immunoaffinity chromatography or immunoextraction, immobilized metal-ion affinity chromatography, dye-ligand affinity chromatography, chiral separations and biointeraction studies. Examples are presented from fields that include analytical chemistry, pharmaceutical analysis, clinical testing and biotechnology. Current trends and possible future directions in AMC are also discussed.

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Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

Section: Other Types Of Monoliths For Amcmentioning

confidence: 99%

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Affinity monolith chromatography: a review of principles and recent analytical applications

et al. 2012

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“…It is based on specific and reversible binding ability of molecules in solution to complementary molecules, the ligands, usually immobilized on a solid chromatographic media, such as agarose gels, membranes, monoliths, cryogels or alternative novel support materials (Labrou and Clonis 1994;Serpa et al 2005;Tscheliessnig and Jungbauer 2009;Prasanna and Vijayalakshmi 2010;Gondim et al 2012;Dogan et al 2012;Rajak et al 2012;BorsoiRibeiro et al 2013;Pavan et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Prediction of the isotherms of human IgG adsorption on Ni(II)-IDA-PEVA membrane using artificial neural networks

Schmitz

Bresolin

2014

Adsorption

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The use of artificial neural networks (ANNs) to predict the adsorption isotherms of human immunoglobulin G on immobilized Ni(II) affinity hollow fiber membranes was studied. Neural networks were trained using the Levenberg-Marquardt algorithm combined with Bayesian regularization technique and experimental data from different temperatures. The resulting neural network demonstrated to be able to interpolate the behavior of the maximum adsorption capacity and equilibrium concentration in the temperature range (4, 37°C) with correlation coefficients higher than 0.96. Results demonstrated to be very similar to those achieved with traditionally Langmuir model adjustment. The advantage of interpolation ability of ANNs was also showed.

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Latest Advances in Cryogel Technology for Biomedical Applications

Memić

Colombani

Eggermont

et al. 2019

Advanced Therapeutics

207

239

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There exists a technological need for advanced materials with improved properties for emerging biomedical applications. Recent developments in macroporous materials have demonstrated their applicability as indispensable tools in biomedical research. Cryogels, which are materials with a macroporous 3D structure, are produced as a result of controlled freezing during polymerization with a highly interconnected polymer network. Cryogels’ interest lies in their ability to address some of the limitations of their hydrogel analogues. In this review, hydrogel and cryogel basic concepts are discussed as a short primer for readers unfamiliar with the cryogels literature. Next, a general overview of the methods for synthesis and characterization of cryogels is provided, highlighting key concepts relevant to cryogels and explaining their unique properties. Finally an in‐depth overview of specific technologies and fields where cryogels have been applied is given. It is argued that the latest advances in cryogel technologies are able to address challenges in bioseparation, tissue engineering, and other emerging bioengineering disciplines.

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Evaluation of human interferon adsorption performance of Cibacron Blue F3GA attached cryogels and interferon purification by using FPLC system

Cited by 34 publications

References 37 publications

Affinity monolith chromatography: a review of principles and recent analytical applications

Affinity monolith chromatography: a review of principles and recent analytical applications

Prediction of the isotherms of human IgG adsorption on Ni(II)-IDA-PEVA membrane using artificial neural networks

Latest Advances in Cryogel Technology for Biomedical Applications

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