Study of amino ligands fixation to macroporous supports and their influence on albumin adsorption

Abstract

Heterogeneous networks of ethylene glycol dimethacrylate and 2-hydroxyethyl methacrylate [poly(EGDMA-co-HEMA)] were synthesized by suspension polymerization using different EGDMA contents and agitation speeds. The networks were actived with epichlorhydrine (Ech) or 1,4-butanediol diglycidyl ether (BDGE), and then hexamethylenediamine (HMDA) or ethylenediamine (EDA) were conjugated to the support by coupling reaction. Here, a higher alkyldiamine concentration and temperature, and a longer reaction time led to higher yields. Amino ligands of the support III were used to analyze their adsorption performance of bovine serum albumin (BSA) from the adsorption kinetic. A more external location of HMDA amino ligands into network led to get the maximum adsorption in a time shorter than that with EDA. Due to its bigger size, the HMDA molecule was attached mostly to the network surface between larger pores, which favored a faster protein adsorption. When derivatives containing BDGE were compared, the EDA ligand displayed a BSA retention higher than that with HMDA, because a shorter separation between the ammonium groups along the spacer arm yielded a stronger electrostatic attraction on the protein. Clearly, the balance obtained between the pores system and the reagents molecular structure used in the formation of Ech-HMDA generated the most efficient BSA adsorption. V V C 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: [6771][6772][6773][6774][6775][6776][6777][6778][6779][6780][6781][6782] 2009

Evaporation Kinetics in Swollen Porous Polymeric Networks

“…A high concentration of EGDMA leads to an increase in the number of pendant vinyl groups, giving rise to an earlier phase separation in the preparation process . In these systems, nuclei are more rigid and present a higher aggregation of small microspheres, resulting in a network with a lower average pore size and more stable pore system. ,, An interesting property of sample C1, previously reported, is that the pore volume can actually decrease upon expansion of the polymer beads due to swelling. This effect is related to a considerable degree of expansion of the primary particle.…”

“…Macroporous polymer beads, a class of polymer beads containing a permanent well-developed porous structure, have a wide range of applications such as support for catalysts, , immobilization of enzymes, , HPLC columns, − adsorbents and liberation of active substances, adsorbents in waste treatments, among others . Because of their porous structure, different solutes can diffuse through the polymeric network and, depending on the interactions between the liquid and the matrix, swelling can be present .…”

Behavior of human immunoglobulin G adsorption onto immobilized Cu(II) affinity hollow‐fiber membranes

“…According to the results presented in Table , it was observed that Cu(II) density and consequently IgG adsorption capacity were higher for epichlorohydrin‐activated membranes for both IDA and TREN chelating ligands. Gomez and Strumia () studied the fixation of hexamethylenediamine and ethylenediamine to macroporous supports activated by epichlorohydrin or bisoxirane. These authors concluded that the performance of the coupling reaction decreased for bisoxirane‐activated supports, as the larger segment of methylene groups in the bisoxirane chain led to a bigger nonspecific interaction with the support.…”

Building of a sulfonyl‐group‐functionalized monomer whose polymer is able to catalyze biodiesel formation

“…On the other hand, the swelling degree of polymer 4 was determined when a dry sample (10 mol %) with a particle size range of 0.2–0.4 mm was left in contact with the reaction mixture in a graduated tube for 24 h at 25°C. The equilibrium volume swelling ratio was determined from the bulk volume of the sample in the swollen equilibrium state relative to that found in the dry state . After centrifugation of the heterogeneous mixture, the supernatant was eliminated, and the swollen polymer mass was recorded in a digital balance (Shimadzu LIBROR EB‐3200D, Japan).…”