Synergetic effect of soluble whey protein hydrolysate and Panax ginseng berry extract on muscle atrophy in hindlimb-immobilized C57BL/6 mice

Han, Min Ji; Shin, Ji Eun; Park, Seok Jun; Choung, Se‐Young

doi:10.1016/j.jgr.2021.06.010

Cited by 7 publications

(7 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Various models of sarcopenia have been previously used, such as denervation, cast immobilization, and hindlimb unloading [38]. Previous studies have shown that hindlimbimmobilization significantly reduced muscle mass, exercise endurance, grip strength, and muscle fiber CSA [43][44][45].…”

Section: Discussionmentioning

confidence: 99%

“…Activation of the PI3K/Akt pathway increases protein turnover through simultaneous regulation of protein anabolism and catabolism. First, the PI3K/Akt/mTORc1 pathway regulates muscle protein synthesis [38]. The phosphorylation ratio of PI3K and Akt was significantly decreased during IM, which was followed by a significantly reduced phosphorylation ratio of mTOR, S6K1, and 4E-BP1.…”

Section: Tgpn and Pny Stimulated Muscle Protein Synthesis And Blocked Muscle Protein Degradation Via Pi3k/akt Pathway In Immobilization-imentioning

confidence: 99%

See 1 more Smart Citation

Oyster Hydrolysates Attenuate Muscle Atrophy via Regulating Protein Turnover and Mitochondria Biogenesis in C2C12 Cell and Immobilized Mice

Jeon

Choung

2021

Nutrients

Self Cite

View full text Add to dashboard Cite

Sarcopenia, also known as skeletal muscle atrophy, is characterized by significant loss of muscle mass and strength. Oyster (Crassostrea gigas) hydrolysates have anti-cancer, antioxidant, and anti-inflammation properties. However, the anti-sarcopenic effect of oyster hydrolysates remains uninvestigated. Therefore, we prepared two different oyster hydrolysates, namely TGPN and PNY. This study aimed to determine the anti-muscle atrophy efficacy and molecular mechanisms of TGPN and PNY on both C2C12 cell lines and mice. In vitro, the TGPN and PNY recovered the dexamethasone-induced reduction in the myotube diameters. In vivo, TGPN and PNY administration not only improved grip strength and exercise endurance, but also attenuated the loss of muscle mass and muscle fiber cross-sectional area. Mechanistically, TGPN and PNY increased the expression of protein synthesis-related protein levels via phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of the rapamycin pathway, and reduced the expression of protein degradation-related protein levels via the PI3K/Akt/forkhead box O pathway. Also, TGPN and PNY stimulated NAD-dependent deacetylase sirtuin-1(SIRT1), peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α), nuclear respiratory factor 1,2, mitochondrial transcription factor A, along with mitochondrial DNA content via SIRT1/PGC-1α signaling. These findings suggest oyster hydrolysates could be used as a valuable natural material that inhibits skeletal muscle atrophy via regulating protein turnover and mitochondrial biogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Tgpn and Pny Stimulated Muscle Protein Synthesis And Blocked Muscle Protein Degradation Via Pi3k/akt Pathway In Immobilization-imentioning

confidence: 99%

Oyster Hydrolysates Attenuate Muscle Atrophy via Regulating Protein Turnover and Mitochondria Biogenesis in C2C12 Cell and Immobilized Mice

Jeon

Choung

2021

Nutrients

Self Cite

View full text Add to dashboard Cite

show abstract

“…The GBE and WPH mixture preparation were performed as previously described [18]. Briefly, whey protein concentrate was dissolved in distilled water to a 20% concentration and then pH was adjusted from 7 to 7.5.…”

Section: Preparation Of Gbe and Wphmentioning

confidence: 99%

“…Mice were adapted to the environment for a week and divided into the control group and the GBE + WPH mixture administration groups (n = 10 per group); L group (700 mg/kg GBE + WPH mixture), M group (900 mg/kg GBE + WPH mixture), and H group (1100 mg/kg GBE + WPH mixture). The ratio of WPH to GBE in the mixture is 8:1, which was selected as the optimal ratio following a previous study [18]. Oral administration was conducted for 8 weeks, and we measured body weight and grip strength twice a week during the experiment.…”

Section: Mice and Design Of Experimentsmentioning

confidence: 99%

“…In a previous study, we confirmed the synergetic effect of a GBE and WPH mixture as a supplement for muscle atrophy in the immobilization (IM)induced muscle atrophy mouse model. We also found the optimal mixing ratio comparing three different mixing ratios of GBE and WPH [18]. Therefore, in this study, we aimed to investigate whether the GBE and WPH mixture also effective in 10-months old mice and to examine aging-related mechanism changes in the skeletal muscle.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Panax ginseng Berry Extract and Soluble Whey Protein Hydrolysate Mixture Ameliorates Sarcopenia-Related Muscular Deterioration in Aged Mice

Han

Park²,

Lee³

et al. 2022

Nutrients

Self Cite

View full text Add to dashboard Cite

Sarcopenia is prevalent as the aging population grows. Therefore, the need for supplements for the elderly is increasing. This study aimed to investigate the efficacy and mechanism of a Panax ginseng berry extract (GBE) and soluble whey protein hydrolysate (WPH) mixture on a sarcopenia-related muscular deterioration in aged mice. Ten-month-old male C57BL/6J mice were administered three different doses of the GBE + WPH mixture for 8 weeks; 700 mg/kg, 900 mg/kg, and 1100 mg/kg. Grip strength, serum inflammatory cytokines level, and mass of muscle tissues were estimated. The deteriorating function of aging muscle was investigated via protein or gene expression. Grip strength and mass of three muscle tissues were increased significantly in a dose-dependent manner, and increased anti-inflammatory cytokine alleviated systemic inflammatory state. The mixture resolved the imbalance of muscle protein turnover through activation of the PI3K/Akt pathway and increased gene expression of the muscle regeneration-related factors, while decreasing myostatin, which interferes with muscle protein synthesis and regeneration. Furthermore, we confirmed that increased mitochondria number in muscle with the improvement of mitochondrial biogenesis. These physiological changes were similar to the effects of exercise.

show abstract

Therapeutic applications of ginseng for skeletal muscle-related disorder management

Ahmad

Chun

Ahmad

et al. 2024

Journal of Ginseng Research

View full text Add to dashboard Cite

Synergetic effect of soluble whey protein hydrolysate and Panax ginseng berry extract on muscle atrophy in hindlimb-immobilized C57BL/6 mice

Cited by 7 publications

References 27 publications

Oyster Hydrolysates Attenuate Muscle Atrophy via Regulating Protein Turnover and Mitochondria Biogenesis in C2C12 Cell and Immobilized Mice

Oyster Hydrolysates Attenuate Muscle Atrophy via Regulating Protein Turnover and Mitochondria Biogenesis in C2C12 Cell and Immobilized Mice

The Panax ginseng Berry Extract and Soluble Whey Protein Hydrolysate Mixture Ameliorates Sarcopenia-Related Muscular Deterioration in Aged Mice

Therapeutic applications of ginseng for skeletal muscle-related disorder management

Contact Info

Product

Resources

About